FastQCFastQC Report
Mon 15 Aug 2016
SRR2921000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416862
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG32700.029.8400654
GTACATG65300.028.7974641
TACATGG65300.028.1545752
GTATCAA37050.027.7867811
ACATGGG63600.027.4103263
TCAACGC41050.023.3354634
CAACGCA42950.022.164635
ATCAACG42700.022.155063
TATCAAC43450.022.0465072
ATGGGGA20400.021.8743325
AACGCAG43250.021.8725476
ACCGCGT1450.00386928820.5158928
ACGCAGA48250.019.112737
GTATAGG4709.094947E-1218.9921321
CATGGGT11050.018.845584
TATACGG1908.324704E-418.78895
CGCAGAG49250.018.4830478
GTGTAGC8650.017.8870031
GTATATG6500.017.3948441
GAGTACA47000.017.2195341
Produced by FastQC (version 0.11.5)