FastQCFastQC Report
Mon 15 Aug 2016
SRR2921000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416862
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23950.16903551651466409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.12012461340624563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.10332692951042514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48950.036.035271
GGTATCA33950.032.999461
TCAACGC59900.029.4374474
ATCAACG60850.029.2705213
TATCAAC60150.029.2129612
CAACGCA60500.029.1043555
AACGCAG63150.028.072426
ACGCAGA71950.024.4753287
CGCAGAG73200.023.895678
CATGGGG34750.023.4855634
ACATGGG61400.023.187263
GTACATG67050.022.8414211
TACATGG65600.022.7907732
AGTACTT44800.021.51621812-13
GCAGAGT81400.020.7575879
CCGTATA1450.003869567820.5156359
GACCGAC1754.7878397E-420.3976827
GAGTACT40000.020.30493512-13
TCTATAC5350.020.0418873
AGGACCG4750.020.0377065
Produced by FastQC (version 0.11.5)