##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1416862
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.359443615539128	31.0	30.0	34.0	27.0	34.0
2	30.403206522583005	31.0	30.0	34.0	27.0	34.0
3	30.54625291665667	31.0	30.0	34.0	27.0	34.0
4	34.13832328060178	35.0	35.0	37.0	30.0	37.0
5	33.795972367104206	35.0	35.0	37.0	28.0	37.0
6	33.62997807831673	36.0	35.0	37.0	28.0	37.0
7	33.48536695881462	36.0	35.0	37.0	28.0	37.0
8	33.42710722709763	36.0	35.0	37.0	27.0	37.0
9	34.775974653847726	38.0	35.0	39.0	27.0	39.0
10-11	34.70032367301826	38.0	34.5	39.0	27.0	39.0
12-13	34.625557746625994	38.0	34.0	39.0	27.0	39.0
14-15	35.5665347083908	39.0	34.0	41.0	27.0	41.0
16-17	35.58776930992574	39.0	34.5	41.0	27.0	41.0
18-19	35.63928844164075	39.0	35.0	41.0	27.0	41.0
20-21	35.613902765406934	39.0	34.5	41.0	27.0	41.0
22-23	35.588393929684045	39.0	34.0	41.0	27.0	41.0
24-25	35.576554032785126	39.0	34.5	41.0	27.0	41.0
26-27	35.44924488058823	39.0	34.0	41.0	27.0	41.0
28-29	35.32836331272911	39.0	34.0	41.0	26.0	41.0
30-31	35.15703399484212	39.0	34.0	41.0	25.0	41.0
32-33	35.01457022631703	38.5	34.0	41.0	25.0	41.0
34-35	34.89543724088867	38.0	34.0	41.0	25.0	41.0
36-37	34.74624275335213	38.0	34.0	40.5	24.0	41.0
38-39	34.61730006168561	38.0	33.5	40.0	24.0	41.0
40-41	34.435673692991976	38.0	33.0	40.0	23.5	41.0
42-43	34.273542518608025	38.0	33.0	40.0	23.0	41.0
44-45	34.05237842499834	38.0	33.0	40.0	21.5	41.0
46-47	33.88616710731179	38.0	33.0	40.0	20.5	41.0
48-49	33.67708146594376	38.0	33.0	40.0	19.0	41.0
50-51	33.48499571588482	38.0	32.5	40.0	17.5	41.0
52-53	33.25671660331069	38.0	32.0	40.0	15.5	41.0
54-55	33.08047749180936	37.5	32.0	40.0	14.5	41.0
56-57	32.95489433692201	37.0	32.0	40.0	14.0	41.0
58-59	32.898820068574075	37.0	32.0	40.0	13.0	41.0
60-61	32.63795627238221	37.0	31.5	40.0	12.0	41.0
62-63	32.387603732755906	36.0	31.0	40.0	9.0	41.0
64-65	32.1226777907799	36.0	31.0	39.0	8.0	41.0
66-67	31.815844803516505	35.5	31.0	39.0	7.5	41.0
68-69	31.506289603362923	35.0	31.0	39.0	7.0	40.5
70-71	31.162882482556522	35.0	30.0	38.0	4.0	40.0
72-73	30.86684588901389	35.0	30.0	37.0	2.0	40.0
74-75	30.603402095616936	35.0	30.0	37.0	2.0	39.0
76-77	30.270060528124827	35.0	30.0	36.0	2.0	39.0
78-79	29.94443036795397	34.5	29.5	36.0	2.0	38.5
80-81	29.673153066424256	34.0	29.0	35.5	2.0	37.0
82-83	29.404716196778516	34.0	29.0	35.0	2.0	37.0
84-85	29.196400566886542	34.0	29.0	35.0	2.0	36.5
86-87	28.951171673740987	34.0	29.0	35.0	2.0	36.0
88-89	28.8056440923675	34.0	29.0	35.0	2.0	36.0
90-91	28.614910273548166	34.0	29.0	35.0	2.0	35.5
92-93	28.74102488456886	34.0	29.0	35.0	2.0	35.0
94-95	28.677243090717376	34.0	29.0	35.0	2.0	35.0
96-97	28.614109207530447	34.0	29.0	35.0	2.0	35.0
98-99	28.551957071330868	34.0	29.0	35.0	2.0	35.0
100-101	28.42641061726548	34.0	29.0	35.0	2.0	35.0
102-103	27.10401965752487	32.0	26.0	34.0	2.0	35.0
104-105	28.171911590543047	34.0	29.0	35.0	2.0	35.0
106-107	28.374026193094316	34.0	29.0	35.0	2.0	35.0
108-109	28.354009070749306	34.0	29.0	35.0	2.0	35.0
110-111	28.296776962047115	34.0	29.0	35.0	2.0	35.0
112-113	28.194883834840653	34.0	29.0	35.0	2.0	35.0
114-115	28.107582813287394	34.0	29.0	35.0	2.0	35.0
116-117	27.979277092617345	34.0	29.0	35.0	2.0	35.0
118-119	27.85460263596596	34.0	29.0	35.0	2.0	35.0
120-121	27.72054794327182	34.0	29.0	35.0	2.0	35.0
122-123	27.49742811932284	34.0	29.0	35.0	2.0	35.0
124-125	26.402275592118357	33.0	25.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31358.0
3	10831.0
4	5484.0
5	4390.0
6	4969.0
7	6110.0
8	7189.0
9	8017.0
10	8584.0
11	9268.0
12	10166.0
13	11239.0
14	12549.0
15	10362.0
16	7758.0
17	7105.0
18	6653.0
19	6213.0
20	6179.0
21	6359.0
22	7292.0
23	9608.0
24	12732.0
25	16853.0
26	20817.0
27	24954.0
28	29469.0
29	34670.0
30	41334.0
31	50216.0
32	61060.0
33	75557.0
34	96991.0
35	133095.0
36	211082.0
37	300313.0
38	109681.0
39	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70295609044452	20.281309822823818	13.440279742204453	24.575454344527213
2	16.073734982616468	21.065484332233716	38.438784973511815	24.421995711638004
3	19.057543806652923	25.155220979481964	29.571634561432358	26.21560065243275
4	13.237336152429032	16.704060670859036	36.031237125568246	34.027366051143694
5	13.435969900739636	37.9380270317389	34.53460278561441	14.091400281907058
6	32.820170055327615	36.750061581004964	16.735917359350044	13.693851004317375
7	28.13284773540559	32.33574014864378	21.360732914081634	18.170679201868985
8	26.22767187547423	36.37775301090441	19.341783087833072	18.052792025788293
9	26.213364016263835	15.88342839394624	19.553450417890218	38.349757171899704
10-11	25.25749035580293	26.26747514391295	27.695381020784875	20.779653479499245
12-13	25.87767024764481	23.501179403863674	28.06134100142912	22.559809347062394
14-15	22.82947292027814	24.971275900665976	25.554688390733883	26.644562788322006
16-17	22.779677314821026	27.979274611398964	26.377285945551733	22.86376212822828
18-19	22.85373690278426	26.820443270285022	28.4079235993101	21.917896227620627
20-21	23.55278247324759	26.113149899986198	28.096730482307596	22.237337144458614
22-23	22.972920981024533	25.918691289582817	28.07588492943543	23.032502799957218
24-25	22.81411164405828	26.23768439652352	28.21396967907513	22.734234280343074
26-27	22.636888269240952	26.325291636517655	28.24290983534229	22.794910258899108
28-29	22.72774602076491	26.449899316836945	28.196474236228642	22.625880426169502
30-31	22.93401104816364	26.367307318967192	28.043945571027688	22.65473606184148
32-33	22.66362447150448	26.3283071694666	28.06068529870013	22.94738306032879
34-35	22.710106154849193	26.561091898619697	28.114179736436075	22.614622210095035
36-37	22.989942076839526	26.21036248835554	28.025363489100002	22.774331945704933
38-39	22.964503867453242	26.330098924342504	27.889258674588895	22.816138533615355
40-41	22.928359928664925	26.338803485421636	28.04289565056544	22.689940935347998
42-43	23.026708493959763	26.266158686499686	27.823421707433216	22.883711112107335
44-45	23.024137710720932	26.383388640757545	27.702789280302216	22.88968436821931
46-47	23.03966766872491	26.51254784957548	27.55422481216703	22.893559669532582
48-49	23.019302031863425	26.739890277539512	27.25631017103629	22.98449751956077
50-51	22.999340299311392	26.842829222940356	27.19286944385714	22.964961033891107
52-53	23.163278632973412	26.75274707819598	27.0628059908013	23.021168298029306
54-55	23.09590878604963	26.74280772353419	27.05743231317731	23.10385117723887
56-57	22.951302273520678	26.868412255952773	27.045820924551194	23.13446454597535
58-59	22.885157690340485	26.666393692315783	27.203317767912267	23.245130849431465
60-61	22.958313226728023	26.739284606100572	27.31214194210215	22.99026022506925
62-63	22.808702774071016	26.746520937917012	27.424223168329416	23.02055311968256
64-65	22.674984035156896	26.706102506118533	27.29816961176396	23.32074384696061
66-67	22.721003665964773	26.853006638273236	27.122526532795117	23.30346316296687
68-69	23.03176761451322	26.54206854485151	27.20152293653113	23.224640904104135
70-71	22.84944338700841	26.50767001971161	27.24710256250953	23.395784030770447
72-73	22.993345688818287	26.598746800811934	27.090142088076956	23.317765422292823
74-75	22.926711668273867	26.72649310786408	26.967467254765477	23.379327969096575
76-77	22.821410532312505	26.50754695603342	27.177512919461133	23.493529592192942
78-79	22.939079992785143	26.59331326774779	27.102871364818675	23.36473537464839
80-81	22.928053155695867	26.660974304597612	27.04349679989947	23.36747573980705
82-83	22.89240835644881	26.688406630995054	27.010164221685507	23.409020790870628
84-85	22.717221150571014	26.76887454268535	26.89682557891226	23.61707872783138
86-87	22.76382471846495	26.611508635341735	27.03103583507092	23.593630811122395
88-89	22.763772498525427	26.605822269458834	26.794525557364775	23.835879674650965
90-91	22.645990953816742	26.717514678692478	26.969578025977953	23.66691634151283
92-93	22.69376073712965	26.729350736999052	27.016497808187168	23.56039071768413
94-95	22.555907182676133	26.55763245373217	27.106839033763496	23.7796213298282
96-97	22.756742962848186	26.276915996079364	27.12178717237285	23.8445538686996
98-99	22.648658093004297	26.451636814155542	26.946360782112848	23.953344310727314
100-101	22.57930957072924	26.42092233014849	26.95408256730155	24.04568553182072
102-103	22.52855200495857	26.352664030191917	27.00947662540645	24.109307339443063
104-105	22.537183889728354	26.399035137227163	26.886584503674182	24.1771964693703
106-107	22.383540970044265	26.54192228605795	26.80242994599257	24.27210679790521
108-109	22.36149955034384	26.512171775711224	26.863143583611336	24.263185090333593
110-111	22.3697697171973	26.56264338849334	26.843667187630153	24.223919706679204
112-113	22.20513993359084	26.616570384676198	26.804616735560526	24.373672946172437
114-115	22.1821875451096	26.78566953850476	26.669200745259104	24.36294217112654
116-117	22.127727306897267	26.709881708616457	26.721775433070977	24.440615551415302
118-119	22.177280322988775	26.579061137413163	26.626493055494286	24.61716548410378
120-121	22.03522739704756	26.809771350124755	26.64344524084289	24.511556011984798
122-123	21.958142499295953	26.774430109393872	26.619610151559105	24.647817239751074
124-125	21.896725726225004	26.87871769700551	26.575671669661826	24.648884907107657
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	43.0
2	98.0
3	181.0
4	162.0
5	128.5
6	182.5
7	397.5
8	642.0
9	659.0
10	526.0
11	427.0
12	438.0
13	488.0
14	546.0
15	636.5
16	734.5
17	821.0
18	995.0
19	1165.5
20	1386.0
21	1697.0
22	2108.0
23	2633.5
24	3228.0
25	4189.0
26	5753.5
27	7662.5
28	9390.0
29	11480.5
30	14078.5
31	17168.5
32	20632.0
33	23318.0
34	25551.0
35	27869.5
36	30641.5
37	33656.0
38	36343.5
39	38513.0
40	40339.0
41	42227.5
42	44038.0
43	46828.5
44	49699.5
45	51899.5
46	54082.5
47	55132.0
48	56138.5
49	57618.5
50	58993.5
51	58255.5
52	54921.0
53	52457.0
54	50532.5
55	47097.5
56	43120.5
57	39246.0
58	35884.0
59	31950.5
60	27097.5
61	22929.0
62	18624.5
63	13618.0
64	9709.0
65	7365.0
66	5321.0
67	3828.5
68	2790.5
69	2065.5
70	1635.0
71	1264.0
72	977.0
73	805.5
74	625.5
75	444.0
76	288.5
77	171.5
78	98.0
79	51.0
80	29.0
81	20.0
82	12.5
83	7.0
84	3.5
85	2.0
86	3.5
87	2.5
88	3.0
89	3.5
90	1.5
91	1.0
92	1.0
93	1.0
94	2.5
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04947553113853007
2	2.823140150558064E-4
3	7.763635414034677E-4
4	0.12358296009067926
5	0.005858015812407983
6	0.0020467766091545967
7	0.0036700821957254834
8	0.006704957857575402
9	0.015668427835597257
10-11	0.014609750279137983
12-13	0.018103386215453588
14-15	0.026008178637016165
16-17	0.03070164913731895
18-19	0.03070164913731895
20-21	0.021702889907415118
22-23	0.022761567463874394
24-25	0.02226751793752673
26-27	0.02025603058025411
28-29	0.02043247683966399
30-31	0.02378495576845169
32-33	0.024067269783507497
34-35	0.027772641231114958
36-37	0.029395946817685845
38-39	0.030066442603443384
40-41	0.03161916968625032
42-43	0.02840784776499052
44-45	0.027208013201003346
46-47	0.03133685567119451
48-49	0.026749252926537657
50-51	0.021914625418706972
52-53	0.025196525843730725
54-55	0.029078343550748065
56-57	0.02530239359937665
58-59	0.025937600133252216
60-61	0.032007351456952056
62-63	0.037547764002422254
64-65	0.033066029013411324
66-67	0.032077929960716005
68-69	0.027384459460413225
70-71	0.030913384648610806
72-73	0.034371731333044435
74-75	0.022585121204464514
76-77	0.0209971048697756
78-79	0.024031980531625523
80-81	0.024279005294799354
82-83	0.029854707092151527
84-85	0.021491154396123265
86-87	0.027278591704767294
88-89	0.024878922576792944
90-91	0.024914211828674915
92-93	0.024031980531625523
94-95	0.019267931527558787
96-97	0.018032807711689636
98-99	0.022196939433762778
100-101	0.02177346841117907
102-103	0.02286743521952032
104-105	0.020291319832136088
106-107	0.014468593271610078
108-109	0.01580958484312516
110-111	0.01806809696357161
112-113	0.013409915715150805
114-115	0.012245370403045604
116-117	0.010480907808946813
118-119	0.006669668605693427
120-121	0.004728759752184758
122-123	0.0038818177070173384
124-125	0.028972475795102134
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1416862.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.39562359101183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7191529361892	42.311567533737495
2	14.104158307290385	16.19033922546563
3	5.262790019544257	9.061833450008878
4	2.5194824706909036	5.784290701277103
5	1.346277098133695	3.8635206786840626
6	0.8024648301928381	2.7634781603264007
7	0.5100003614291088	2.049025214310558
8	0.3425132110139578	1.572700746744474
9	0.2512616570881535	1.2979187543776993
>10	1.0708134616831029	10.40066230174277
>50	0.04981364195951298	1.9812078764183376
>100	0.02040631326108637	2.1069118491354564
>500	2.4734043534149145E-4	0.07176208684739956
>1k	6.183510883537287E-4	0.5447814209236316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2395	0.16903551651466409	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1702	0.12012461340624563	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1464	0.10332692951042514	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.05785037639516E-5
2	0.0	0.0	0.0	0.0	7.05785037639516E-5
3	0.0	0.0	0.0	0.0	7.05785037639516E-5
4	0.0	0.0	0.0	0.0	7.05785037639516E-5
5	0.0	0.0	0.0	7.05785037639516E-5	7.05785037639516E-5
6	0.0	0.0	0.0	7.05785037639516E-5	7.05785037639516E-5
7	0.0	0.0	0.0	7.05785037639516E-5	7.05785037639516E-5
8	0.0	0.0	0.0	7.05785037639516E-5	7.05785037639516E-5
9	0.0	0.0	0.0	7.05785037639516E-5	7.05785037639516E-5
10-11	0.0	0.0	0.0	1.411570075279032E-4	7.05785037639516E-5
12-13	0.0	0.0	0.0	1.411570075279032E-4	1.411570075279032E-4
14-15	0.0	0.0	0.0	2.823140150558064E-4	1.7644625940987902E-4
16-17	0.0	0.0	0.0	4.234710225837096E-4	2.117355112918548E-4
18-19	0.0	0.0	0.0	4.234710225837096E-4	2.823140150558064E-4
20-21	0.0	0.0	0.0	4.5876027446568543E-4	4.234710225837096E-4
22-23	0.0	0.0	0.0	7.763635414034676E-4	4.234710225837096E-4
24-25	0.0	0.0	0.0	0.0010586775564592741	4.234710225837096E-4
26-27	0.0	0.0	0.0	0.001658594838452863	4.234710225837096E-4
28-29	0.0	0.0	0.0	0.0036700821957254834	4.234710225837096E-4
30-31	0.0	0.0	0.0	0.011010246587176451	4.234710225837096E-4
32-33	0.0	0.0	0.0	0.02664338517089173	5.646280301116128E-4
34-35	0.0	0.0	0.0	0.04569958118715867	5.646280301116128E-4
36-37	0.0	0.0	0.0	0.07481321398978871	5.646280301116128E-4
38-39	0.0	0.0	0.0	0.12460634839525656	5.646280301116128E-4
40-41	0.0	0.0	0.0	0.18248072148169686	5.646280301116128E-4
42-43	0.0	0.0	0.0	0.23784955768451693	5.646280301116128E-4
44-45	0.0	0.0	0.0	0.30341698768122793	5.646280301116128E-4
46-47	0.0	0.0	0.0	0.3811944988291026	5.646280301116128E-4
48-49	0.0	0.0	0.0	0.46228919965388304	5.646280301116128E-4
50-51	0.0	0.0	0.0	0.5515357176633998	5.646280301116128E-4
52-53	0.0	0.0	0.0	0.6414880207105561	6.352065338755644E-4
54-55	0.0	0.0	0.0	0.7339105713894507	6.352065338755644E-4
56-57	0.0	0.0	0.0	0.844436508283799	7.763635414034676E-4
58-59	0.0	0.0	0.0	0.9673842618406027	8.469420451674192E-4
60-61	0.0	0.0	0.0	1.1007776339544713	8.469420451674192E-4
62-63	0.0	0.0	0.0	1.2405583606589774	9.175205489313709E-4
64-65	0.0	0.0	0.0	1.384115037314855	9.175205489313709E-4
66-67	0.0	0.0	0.0	1.5522683225324698	9.175205489313709E-4
68-69	0.0	0.0	0.0	1.7419833406499716	9.175205489313709E-4
70-71	0.0	0.0	0.0	1.951566207577026	9.175205489313709E-4
72-73	0.0	0.0	0.0	2.1625959338312413	9.528098008133466E-4
74-75	0.0	0.0	0.0	2.3806129319580878	9.880990526953224E-4
76-77	0.0	0.0	0.0	2.622556042860914	9.880990526953224E-4
78-79	0.0	0.0	0.0	2.895306670656705	9.880990526953224E-4
80-81	0.0	0.0	0.0	3.183902172547503	9.880990526953224E-4
82-83	0.0	0.0	0.0	3.490071721875525	0.0010586775564592741
84-85	0.0	0.0	0.0	3.805451765944743	0.0010586775564592741
86-87	0.0	0.0	0.0	4.136429659345794	0.0010586775564592741
88-89	0.0	0.0	0.0	4.494050937917737	0.0010939668083412499
90-91	0.0	0.0	0.0	4.884632377747445	0.0011292560602232256
92-93	0.0	0.0	0.0	5.294058278082128	0.001270413067751129
94-95	0.0	0.0	0.0	5.721975746402967	0.001270413067751129
96-97	0.0	0.0	0.0	6.158397924427361	0.0013409915715150806
98-99	0.0	0.0	0.0	6.62365142123933	0.0014115700752790322
100-101	0.0	0.0	0.0	7.150590530340994	0.0014115700752790322
102-103	0.0	0.0	0.0	7.686281373909385	0.001446859327161008
104-105	0.0	0.0	0.0	8.2144908960788	0.0014821485790429837
106-107	0.0	0.0	0.0	8.747499756504162	0.0014821485790429837
108-109	0.0	0.0	0.0	9.317068281879251	0.0014821485790429837
110-111	0.0	0.0	0.0	9.93611233839287	0.0014821485790429837
112-113	0.0	0.0	0.0	10.577776805362838	0.0014821485790429837
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4895	0.0	36.03527	1
GGTATCA	3395	0.0	32.99946	1
TCAACGC	5990	0.0	29.437447	4
ATCAACG	6085	0.0	29.270521	3
TATCAAC	6015	0.0	29.212961	2
CAACGCA	6050	0.0	29.104355	5
AACGCAG	6315	0.0	28.07242	6
ACGCAGA	7195	0.0	24.475328	7
CGCAGAG	7320	0.0	23.89567	8
CATGGGG	3475	0.0	23.485563	4
ACATGGG	6140	0.0	23.18726	3
GTACATG	6705	0.0	22.841421	1
TACATGG	6560	0.0	22.790773	2
AGTACTT	4480	0.0	21.516218	12-13
GCAGAGT	8140	0.0	20.757587	9
CCGTATA	145	0.0038695678	20.515635	9
GACCGAC	175	4.7878397E-4	20.397682	7
GAGTACT	4000	0.0	20.304935	12-13
TCTATAC	535	0.0	20.041887	3
AGGACCG	475	0.0	20.037706	5
>>END_MODULE