FastQCFastQC Report
Mon 15 Aug 2016
SRR2920999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386002
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92100.029.533041
CATGGGG83450.027.2394774
GTACATG143650.026.9314251
TCAACGC101500.026.3234044
CAACGCA103800.025.7382285
AACGCAG104450.025.5195876
TATCAAC108700.025.0196082
TACATGG148600.024.9095232
ACATGGG147950.024.6167263
ATCAACG108600.024.6042613
ACGCAGA118600.022.5250457
CGCAGAG119100.022.0305028
GTATAGG7850.021.9877071
GGTATCA70950.019.9653131
GCAGAGT128500.019.1685129
GTATAAG15550.018.3722531
GTATACG2301.4338183E-418.114311
GTGTACG4752.0190782E-1017.5422781
ATGGGGA48550.016.4227265
GTATATA16700.016.3943021
Produced by FastQC (version 0.11.5)