FastQCFastQC Report
Mon 15 Aug 2016
SRR2920999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386002
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.11518008554041019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111950.034.9647831
GGTATCA83050.028.480031
TCAACGC136200.028.4231154
CAACGCA137800.027.9345155
TATCAAC139050.027.9224952
ATCAACG141450.027.3677673
AACGCAG140950.027.2643896
GTACATG151800.024.059041
CATGGGG86650.023.712974
ACGCAGA160350.023.597257
TACATGG153650.023.1764132
CGCAGAG162250.023.137928
ACATGGG147700.023.0242023
GAGTACT82700.020.50625612-13
GCAGAGT178900.020.2534719
GTACTTT85600.019.63789414-15
AGTACTT88950.019.63401812-13
AGAGTAC162300.018.64164210-11
TACCGTA2702.4516126E-517.6278557
GTATAGA13550.017.1480561
Produced by FastQC (version 0.11.5)