FastQCFastQC Report
Mon 15 Aug 2016
SRR2920998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3946756
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81600.029.9024241
GTACATG142250.029.6205881
TACATGG145450.027.9011972
CATGGGG92650.027.7439614
ACATGGG144200.027.1939533
TCAACGC86900.026.9778044
CAACGCA89300.026.3853515
AACGCAG90550.026.08556
ATCAACG91900.025.7055663
TATCAAC97050.024.4028022
GGTATCA63100.024.1447811
ACGCAGA104600.022.297267
CGCAGAG108100.021.3551758
ATGGGGG52950.018.8782835
ATGGGGA56900.017.9860295
GTATAGT12100.017.7063831
GCAGAGT124300.017.2314979
GTAATAC10200.016.9180373
GTATATA21000.016.7203521
TACCGTA2904.5238146E-516.4130447
Produced by FastQC (version 0.11.5)