##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920998_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3946756 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.30345681364645 31.0 28.0 33.0 16.0 34.0 2 28.419095581282452 31.0 28.0 34.0 16.0 34.0 3 28.44727011246705 31.0 28.0 34.0 16.0 34.0 4 31.92562600778969 35.0 32.0 37.0 19.0 37.0 5 31.95981433866193 35.0 32.0 37.0 19.0 37.0 6 31.93107985393574 35.0 32.0 37.0 19.0 37.0 7 31.81103696301469 35.0 32.0 37.0 18.0 37.0 8 31.817409791737823 35.0 32.0 37.0 18.0 37.0 9 32.9680712463603 37.0 32.0 39.0 17.0 39.0 10-11 32.90413544693414 37.0 32.0 39.0 17.0 39.0 12-13 32.81781924192932 37.0 32.0 39.0 17.0 39.0 14-15 33.68740428848401 38.0 32.0 40.0 17.0 41.0 16-17 33.540235449062465 38.0 32.0 40.0 16.0 41.0 18-19 33.63942906528805 38.0 32.0 40.0 16.5 41.0 20-21 33.65634308277482 38.0 32.0 40.0 16.0 41.0 22-23 33.59607548579137 38.0 32.0 40.0 16.0 41.0 24-25 33.50741849762184 38.0 32.0 40.0 15.5 41.0 26-27 33.34240411619061 38.0 32.0 40.0 12.0 41.0 28-29 33.25270665326157 38.0 32.0 40.0 10.0 41.0 30-31 33.11994445564915 38.0 32.0 40.0 10.0 41.0 32-33 33.021662220821355 38.0 31.5 40.0 9.0 41.0 34-35 32.83978424305936 38.0 31.0 40.0 9.0 41.0 36-37 32.68810118993928 38.0 31.0 40.0 8.0 41.0 38-39 32.55820032958714 38.0 31.0 40.0 8.0 41.0 40-41 32.474523000661804 38.0 31.0 40.0 4.5 41.0 42-43 32.267494747585104 38.0 30.0 40.0 2.0 41.0 44-45 32.099394667418 37.5 30.0 40.0 2.0 41.0 46-47 31.910927480695538 37.0 30.0 40.0 2.0 41.0 48-49 31.752959772532176 37.0 30.0 40.0 2.0 41.0 50-51 31.526007181594203 37.0 30.0 40.0 2.0 41.0 52-53 31.344420835744597 37.0 30.0 40.0 2.0 41.0 54-55 31.133315310092645 36.0 29.0 40.0 2.0 41.0 56-57 30.931919024130195 36.0 29.0 40.0 2.0 41.0 58-59 30.678825850901347 36.0 29.0 39.0 2.0 41.0 60-61 30.491516577158556 35.0 28.5 39.0 2.0 41.0 62-63 30.379114391667486 35.0 28.0 39.0 2.0 41.0 64-65 30.160727949738977 35.0 28.0 39.0 2.0 41.0 66-67 29.906896575314004 35.0 28.0 39.0 2.0 40.0 68-69 29.605814876825423 35.0 28.0 38.0 2.0 40.0 70-71 29.277497899540787 34.5 28.0 37.5 2.0 40.0 72-73 29.00018280836211 34.0 28.0 37.0 2.0 39.5 74-75 28.692933006246143 34.0 27.5 36.5 2.0 39.0 76-77 28.40421728629791 34.0 27.0 36.0 2.0 39.0 78-79 28.079868631351925 34.0 26.0 35.5 2.0 37.5 80-81 27.748204348077255 34.0 26.0 35.0 2.0 37.0 82-83 27.464409758292632 34.0 26.0 35.0 2.0 37.0 84-85 27.207861975759332 33.0 26.0 35.0 2.0 36.0 86-87 27.12636035772163 33.0 26.0 35.0 2.0 36.0 88-89 27.203087041610885 34.0 26.0 35.0 2.0 36.0 90-91 27.10323681018031 34.0 26.0 35.0 2.0 35.0 92-93 26.94225624791601 34.0 25.5 35.0 2.0 35.0 94-95 26.844706893458827 34.0 25.0 35.0 2.0 35.0 96-97 26.737805808111776 33.0 25.0 35.0 2.0 35.0 98-99 26.66214924358131 33.0 25.0 35.0 2.0 35.0 100-101 26.481576134932084 33.0 25.0 35.0 2.0 35.0 102-103 25.159678480250616 31.5 21.5 34.0 2.0 35.0 104-105 26.154722638034883 33.0 24.0 35.0 2.0 35.0 106-107 26.33215797987005 33.0 24.0 35.0 2.0 35.0 108-109 26.306452945152934 33.0 24.0 35.0 2.0 35.0 110-111 26.183825653270688 33.0 24.0 35.0 2.0 35.0 112-113 26.076482559347475 33.0 24.0 35.0 2.0 35.0 114-115 25.93039334075884 33.0 23.0 35.0 2.0 35.0 116-117 25.784676579955793 33.0 23.0 35.0 2.0 35.0 118-119 25.5662621403502 33.0 19.5 35.0 2.0 35.0 120-121 25.323441834255778 33.0 18.5 35.0 2.0 35.0 122-123 25.069561812283304 33.0 9.0 35.0 2.0 35.0 124-125 23.892932195453685 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 236577.0 3 34087.0 4 21649.0 5 19507.0 6 22002.0 7 24311.0 8 25639.0 9 27118.0 10 28288.0 11 29182.0 12 29372.0 13 28980.0 14 29154.0 15 26731.0 16 23666.0 17 21546.0 18 20305.0 19 19977.0 20 20403.0 21 21958.0 22 26279.0 23 34314.0 24 45131.0 25 56585.0 26 66554.0 27 77281.0 28 89591.0 29 104099.0 30 121069.0 31 144137.0 32 173863.0 33 214303.0 34 274005.0 35 370448.0 36 550653.0 37 682410.0 38 205418.0 39 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.891511209374556 18.4739556390057 12.217433808409432 25.417099343210314 2 15.926014996590302 20.223248440167588 38.750390575801866 25.10034598744025 3 19.357245478236564 24.193629729987954 29.03672987973981 27.412394912035676 4 12.858609452527652 15.90731406794424 36.398696290469445 34.83538018905866 5 13.89420410058129 37.456873297263385 34.46603725437577 14.182885347779559 6 33.31410998206299 35.690516544793496 17.218007753024196 13.777365720119322 7 28.476308384005822 32.03898255352854 21.278695054409052 18.206014008056584 8 26.187173734682222 35.43832372411605 19.98295730102028 18.391545240181443 9 26.2075244086819 16.06796043934994 19.541134770282333 38.18338038168583 10-11 25.08831706268928 26.263741025131658 27.723905481612416 20.924036430566648 12-13 25.51439755701624 23.68943202755971 27.53942716880627 23.25674324661778 14-15 22.74113654563677 25.08338993168797 24.891519292405913 27.283954230269348 16-17 23.12264897212288 27.984028593161135 25.80311847077466 23.09020396394132 18-19 23.12002566158131 27.302840611378056 27.056701757088607 22.520431969952032 20-21 23.86304955392096 26.541272129751864 26.732322170166295 22.863356146160882 22-23 23.50088776080943 26.375661861630935 26.52772455154302 23.59572582601662 24-25 23.202247646518387 26.539562359091352 26.77639276905855 23.481797225331707 26-27 23.103053883840523 26.68322390891456 26.70313922069814 23.510582986546776 28-29 23.20454986851552 26.919092544879998 26.668323762590855 23.208033824013626 30-31 23.381456605124605 26.772526293772437 26.514728933975167 23.331288167127788 32-33 23.104332153334557 26.774498698994652 26.499576677602654 23.621592470068137 34-35 23.372202731707144 26.771170907738274 26.559122606284824 23.29750375426976 36-37 23.444501907908055 26.53986386311409 26.583890561410715 23.431743667567133 38-39 23.310951023103247 26.764681439109662 26.597884622108804 23.32648291567829 40-41 23.433854369432638 26.63988408601118 26.709701563539035 23.216559981017145 42-43 23.334406387548416 26.61448101232467 26.529878225449576 23.521234374677338 44-45 23.411927730350197 26.67617076361403 26.5094500013809 23.402451504654877 46-47 23.378701115973456 26.685579623981116 26.472425551929916 23.463293708115515 48-49 23.243842041878143 26.8026658619317 26.457777639263362 23.495714456926788 50-51 23.24919584082336 26.903717300863228 26.46190487533062 23.385181982982793 52-53 23.41530305944129 26.770675052404524 26.29807781232984 23.51594407582434 54-55 23.299726762387667 26.664915013575737 26.41553248395895 23.619825740077655 56-57 23.207335149173367 26.759625007917908 26.39090390827896 23.64213593462976 58-59 23.277235940356764 26.549883003912704 26.494454135874086 23.678426919856445 60-61 23.222191451296226 26.68404731312807 26.563694423252482 23.530066812323224 62-63 23.118882543569555 26.54815501048933 26.717600967567584 23.61536147837353 64-65 23.088795731465893 26.530265245257883 26.58142623093927 23.79951279233695 66-67 23.133345309422708 26.673622595586803 26.42266947032155 23.770362624668937 68-69 23.29522563853341 26.38113049590257 26.579604025923942 23.744039839640077 70-71 23.090517173664395 26.33917689454579 26.692522055883522 23.877783875906296 72-73 23.148982612742362 26.30804806364022 26.63192027893403 23.911049044683395 74-75 23.082009115713465 26.402262750299204 26.611804819796987 23.903923314190344 76-77 23.130026650018216 26.206940761352353 26.7523691861128 23.910663402516626 78-79 23.104404322799976 26.288912579282055 26.721864015876363 23.884819082041606 80-81 23.199889727825113 26.26332524071045 26.67158820437397 23.865196827090468 82-83 23.113283380429433 26.268893073801202 26.77224807704124 23.84557546872813 84-85 23.07001567885807 26.345739090423532 26.62128463259074 23.96296059812766 86-87 23.032453000210708 26.20249102019163 26.8112516438222 23.953804335775462 88-89 23.080752901876885 26.104768067749816 26.67059985466545 24.14387917570785 90-91 22.919843334230276 26.191791921750223 26.813341927755168 24.075022816264337 92-93 22.95135782178885 26.130432788511946 26.941101972896526 23.977107416802674 94-95 22.854539836237734 26.05736683784307 27.024612990681522 24.06348033523767 96-97 23.005305098261 25.840569343439295 26.97637524733434 24.177750310965365 98-99 22.848638147971442 25.99594701525646 26.85615476746393 24.29926006930817 100-101 22.77914458755366 25.955052435530853 27.004257207343446 24.26154576957205 102-103 22.584447264977168 25.855127776636106 27.099497093215508 24.460927865171218 104-105 22.5006797191322 26.0215614763829 26.96125892345159 24.516499881033315 106-107 22.47557155967848 26.06194041585462 26.917780022206923 24.54470800225997 108-109 22.49012307418635 25.99996351029909 26.937305372424277 24.572608043090284 110-111 22.382299856918472 26.06539479706295 26.997135328365268 24.55517001765331 112-113 22.374969420225206 26.184243349001918 26.97672668516255 24.464060545610327 114-115 22.29870190918217 26.28616484363448 26.861905898013504 24.553227349169845 116-117 22.271843816391318 26.104510172152303 27.03962172381871 24.584024287637675 118-119 22.384803337170414 26.025211214680468 26.89046735943578 24.699518088713333 120-121 22.255848535384512 26.170464080707884 26.965400428407143 24.608286955500468 122-123 22.18017882647234 26.148858031069178 26.95337824372097 24.71758489873751 124-125 22.152907670380362 26.188630340286643 26.84491157049642 24.813550418836567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 725.0 1 559.0 2 354.5 3 280.0 4 240.0 5 223.0 6 216.0 7 237.5 8 280.0 9 315.5 10 343.0 11 406.0 12 459.0 13 512.0 14 578.0 15 646.0 16 767.0 17 930.0 18 1146.0 19 1365.0 20 1728.0 21 2246.0 22 2905.5 23 3765.5 24 5021.0 25 6770.5 26 9301.0 27 13256.5 28 18062.0 29 23831.5 30 30890.0 31 39566.0 32 49879.5 33 58581.5 34 65972.5 35 73903.5 36 82576.0 37 91737.5 38 100839.5 39 107649.5 40 113958.5 41 121583.0 42 127704.0 43 135393.0 44 143055.0 45 148992.5 46 156477.5 47 161377.5 48 162128.0 49 161802.5 50 161815.5 51 161039.5 52 155394.0 53 148900.5 54 143324.0 55 134951.0 56 124876.0 57 114627.5 58 105150.5 59 93418.0 60 78678.0 61 65772.0 62 54219.0 63 41657.5 64 30959.5 65 23638.0 66 18151.0 67 14015.5 68 10743.0 69 8151.5 70 6380.5 71 4935.0 72 3775.5 73 3044.0 74 2343.0 75 1761.0 76 1316.0 77 835.5 78 514.0 79 329.5 80 204.0 81 153.0 82 123.5 83 89.5 84 59.5 85 39.0 86 37.0 87 30.5 88 18.5 89 14.0 90 11.5 91 9.5 92 7.5 93 10.0 94 11.0 95 6.5 96 7.0 97 6.5 98 5.0 99 5.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03428131863231474 2 0.016697257190462243 3 0.02267685157126511 4 0.019889752495467164 5 0.013555436414108194 6 0.004231323142347792 7 0.004307334935324099 8 0.005016778336436304 9 0.002711087282821639 10-11 0.004560707578578458 12-13 0.006853730000030404 14-15 0.0021916733641502034 16-17 0.0019763066173839982 18-19 0.0 20-21 0.003699240591513638 22-23 7.221120332749225E-4 24-25 0.001266863216271794 26-27 0.0011148396303191786 28-29 0.0023436969501028186 30-31 0.0013048691127599478 32-33 1.2668632162717938E-5 34-35 0.006321647449196251 36-37 0.007271794861400097 38-39 9.628160443665634E-4 40-41 9.248101478784095E-4 42-43 0.0019636379852212803 44-45 9.374787800411274E-4 46-47 0.019864415231141727 48-49 0.0029137853974251257 50-51 0.01008423120152348 52-53 0.0013935495378989732 54-55 2.02698114603487E-4 56-57 1.5202358595261525E-4 58-59 0.006499008299474302 60-61 7.347806654376404E-4 62-63 0.009248101478784096 64-65 0.009868864454757275 66-67 7.601179297630762E-5 68-69 0.003395193419608408 70-71 0.004814080221832817 72-73 0.01131308852130712 74-75 0.0013808809057362554 76-77 0.001064165101668307 78-79 2.1536674676620496E-4 80-81 0.00399061913125615 82-83 9.754846765292812E-4 84-85 0.001013490573017435 86-87 0.013720128632223529 88-89 0.0 90-91 2.5337264325435877E-4 92-93 0.00823461090576666 94-95 0.0061442865989182 96-97 0.004434021256951279 98-99 8.868042513902557E-4 100-101 0.015823121571234704 102-103 0.007575842033305327 104-105 0.0069170731608439945 106-107 0.01747004375238804 108-109 0.011072384510215479 110-111 0.01910429730137865 112-113 0.0028251049722861003 114-115 0.013618779574921783 116-117 0.005612204048084047 118-119 0.004535370314253022 120-121 0.007005753585983021 122-123 0.007715196987095225 124-125 0.057211542846834204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3946756.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.48321917028776 #Duplication Level Percentage of deduplicated Percentage of total 1 70.51495938968044 35.598221496531785 2 13.779948911639451 13.913123621233254 3 5.999782543639638 9.086650113738791 4 3.150862528804707 6.362627344683807 5 1.8648514766197652 4.707185290711519 6 1.1665502263505967 3.5334726450003573 7 0.7880798070364431 2.784936393560919 8 0.5444692021169961 2.1989246449555218 9 0.3704895007924371 1.6833152401915592 >10 1.7222820868197775 14.325431764386662 >50 0.06592728009528316 2.257955630478701 >100 0.02952349516257661 2.565952238874531 >500 0.0016672709271713705 0.5785229888988372 >1k 6.062803145766463E-4 0.40368058675380086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.013490573017435E-4 14-15 0.0 0.0 0.0 2.5337264325435876E-5 1.013490573017435E-4 16-17 0.0 0.0 0.0 2.5337264325435876E-5 1.013490573017435E-4 18-19 0.0 0.0 0.0 2.5337264325435876E-5 1.013490573017435E-4 20-21 0.0 0.0 0.0 1.013490573017435E-4 1.013490573017435E-4 22-23 1.2668632162717938E-5 0.0 0.0 3.0404717190523055E-4 1.013490573017435E-4 24-25 2.5337264325435876E-5 0.0 0.0 7.727865619257942E-4 1.013490573017435E-4 26-27 2.5337264325435876E-5 0.0 0.0 0.0014188868022244091 1.013490573017435E-4 28-29 2.5337264325435876E-5 0.0 0.0 0.0041933172458596376 1.013490573017435E-4 30-31 2.5337264325435876E-5 0.0 0.0 0.01581045293907199 1.013490573017435E-4 32-33 3.800589648815381E-5 0.0 0.0 0.04010888942716499 1.013490573017435E-4 34-35 5.067452865087175E-5 0.0 0.0 0.07281929767130271 1.2668632162717938E-4 36-37 5.067452865087175E-5 0.0 0.0 0.12283505744971313 1.2668632162717938E-4 38-39 5.067452865087175E-5 0.0 0.0 0.20368626791217903 1.2668632162717938E-4 40-41 5.067452865087175E-5 0.0 0.0 0.2942669878756123 1.2668632162717938E-4 42-43 5.067452865087175E-5 0.0 0.0 0.3816805497983661 1.2668632162717938E-4 44-45 5.067452865087175E-5 0.0 0.0 0.4863614573589044 1.2668632162717938E-4 46-47 5.067452865087175E-5 0.0 0.0 0.6056619664352192 1.2668632162717938E-4 48-49 5.067452865087175E-5 0.0 0.0 0.7331590805208126 1.3935495378989733E-4 50-51 6.334316081358969E-5 2.5337264325435876E-5 0.0 0.8636966663254582 1.5202358595261525E-4 52-53 7.601179297630762E-5 2.5337264325435876E-5 0.0 1.0006192427401137 1.5202358595261525E-4 54-55 7.601179297630762E-5 2.5337264325435876E-5 0.0 1.1529215386003087 1.5202358595261525E-4 56-57 7.601179297630762E-5 6.334316081358969E-5 0.0 1.3282300704679995 1.646922181153332E-4 58-59 7.601179297630762E-5 7.601179297630762E-5 0.0 1.5184495823912094 1.7736085027805114E-4 60-61 7.601179297630762E-5 7.601179297630762E-5 0.0 1.7138378962368082 1.7736085027805114E-4 62-63 8.868042513902557E-5 1.013490573017435E-4 0.0 1.916675365794085 1.7736085027805114E-4 64-65 1.013490573017435E-4 1.013490573017435E-4 0.0 2.140251386201731 1.7736085027805114E-4 66-67 1.013490573017435E-4 1.013490573017435E-4 0.0 2.39040619688676 1.9002948244076906E-4 68-69 1.013490573017435E-4 1.013490573017435E-4 0.0 2.6659236091615495 2.02698114603487E-4 70-71 1.013490573017435E-4 1.013490573017435E-4 0.0 2.960380626519602 2.02698114603487E-4 72-73 1.013490573017435E-4 1.013490573017435E-4 0.0 3.265681993008942 2.1536674676620496E-4 74-75 1.013490573017435E-4 1.013490573017435E-4 0.0 3.57684893618962 2.4070401109164085E-4 76-77 1.013490573017435E-4 1.013490573017435E-4 0.0 3.925933095433313 2.5337264325435877E-4 78-79 1.013490573017435E-4 1.013490573017435E-4 0.0 4.3101600402963856 2.5337264325435877E-4 80-81 1.013490573017435E-4 1.013490573017435E-4 0.0 4.720598385104122 2.5337264325435877E-4 82-83 1.013490573017435E-4 1.013490573017435E-4 0.0 5.144389468211362 3.040471719052305E-4 84-85 1.013490573017435E-4 1.013490573017435E-4 0.0 5.5865120620580555 3.293844362306664E-4 86-87 1.013490573017435E-4 1.013490573017435E-4 0.0 6.061522424999164 3.293844362306664E-4 88-89 1.013490573017435E-4 1.013490573017435E-4 0.0 6.590526498217777 3.293844362306664E-4 90-91 1.013490573017435E-4 1.013490573017435E-4 0.0 7.14675546195407 3.293844362306664E-4 92-93 1.013490573017435E-4 1.013490573017435E-4 0.0 7.709622788943628 3.293844362306664E-4 94-95 1.013490573017435E-4 1.013490573017435E-4 0.0 8.286501623105153 3.293844362306664E-4 96-97 1.013490573017435E-4 1.013490573017435E-4 0.0 8.898041834863871 3.547217005561023E-4 98-99 1.013490573017435E-4 1.013490573017435E-4 0.0 9.550476391243848 3.547217005561023E-4 100-101 1.1401768946446144E-4 1.013490573017435E-4 0.0 10.255333747513149 3.547217005561023E-4 102-103 1.2668632162717938E-4 1.013490573017435E-4 0.0 10.968615744170656 3.547217005561023E-4 104-105 1.2668632162717938E-4 1.013490573017435E-4 0.0 11.687231234968667 3.547217005561023E-4 106-107 1.2668632162717938E-4 1.013490573017435E-4 0.0 12.41745119282773 3.547217005561023E-4 108-109 1.2668632162717938E-4 1.013490573017435E-4 0.0 13.191491949337633 3.547217005561023E-4 110-111 1.2668632162717938E-4 1.013490573017435E-4 0.0 14.010151628324628 3.547217005561023E-4 112-113 1.2668632162717938E-4 1.013490573017435E-4 0.0 14.843139530287658 3.547217005561023E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8160 0.0 29.902424 1 GTACATG 14225 0.0 29.620588 1 TACATGG 14545 0.0 27.901197 2 CATGGGG 9265 0.0 27.743961 4 ACATGGG 14420 0.0 27.193953 3 TCAACGC 8690 0.0 26.977804 4 CAACGCA 8930 0.0 26.385351 5 AACGCAG 9055 0.0 26.0855 6 ATCAACG 9190 0.0 25.705566 3 TATCAAC 9705 0.0 24.402802 2 GGTATCA 6310 0.0 24.144781 1 ACGCAGA 10460 0.0 22.29726 7 CGCAGAG 10810 0.0 21.355175 8 ATGGGGG 5295 0.0 18.878283 5 ATGGGGA 5690 0.0 17.986029 5 GTATAGT 1210 0.0 17.706383 1 GCAGAGT 12430 0.0 17.231497 9 GTAATAC 1020 0.0 16.918037 3 GTATATA 2100 0.0 16.720352 1 TACCGTA 290 4.5238146E-5 16.413044 7 >>END_MODULE