FastQCFastQC Report
Mon 15 Aug 2016
SRR2920998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3946756
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104000.039.0215451
TCAACGC119850.033.006084
ATCAACG123750.032.1568263
CAACGCA123300.032.1396265
GGTATCA69900.031.6291451
AACGCAG128250.031.078436
TATCAAC130300.030.8564642
ACGCAGA149000.026.5121787
GTACATG146400.026.417661
CGCAGAG152200.025.994518
CATGGGG90400.025.6356964
TACATGG152300.025.1476762
ACATGGG148100.025.0998673
GCAGAGT172350.022.0239419
AGTACTT90550.019.91158712-13
AGAGTAC155800.019.66792110-11
GAGTACT81600.019.36092412-13
ATGGGGA58550.018.394225
TATACTG21650.017.8642655
GTACTTT90000.017.58696414-15
Produced by FastQC (version 0.11.5)