FastQCFastQC Report
Mon 15 Aug 2016
SRR2920997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626257
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86300.034.687161
GTACATG135700.030.7871021
TCAACGC98700.029.539334
TACATGG138750.029.2909432
CAACGCA100150.028.9916295
TATCAAC101750.028.8308982
ACATGGG138050.028.7921283
ATCAACG102800.028.3041063
AACGCAG104050.028.0185556
CATGGGG99400.026.8770544
ACGCAGA119600.024.0771987
GGTATCA60550.023.6872371
CGCAGAG124100.023.0123628
GTATAGG8800.020.288511
AGAGTAC103700.020.19582710-11
GCAGAGT140250.018.9201139
CTATCGG3503.4027835E-818.7040751
ATGGGGA57050.018.1466885
ATGGGGG50800.016.982755
GTATAGA12900.016.1469271
Produced by FastQC (version 0.11.5)