FastQCFastQC Report
Mon 15 Aug 2016
SRR2920997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626257
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45100.12437066650267756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116200.039.5788841
GGTATCA77250.037.2478371
TCAACGC144700.031.4935264
TATCAAC146750.031.0480082
CAACGCA146650.030.9964625
ATCAACG148800.030.664953
AACGCAG153250.029.9295946
GTACATG136400.027.0351431
ACGCAGA172550.026.307197
CGCAGAG176100.025.7782868
TACATGG139650.025.3335442
ACATGGG137450.024.702823
AGAGTAC152650.023.19172110-11
AGTACTT95300.022.758712-13
GCAGAGT194950.022.645489
CATGGGG97550.022.4723744
GAGTACT90500.022.3549212-13
GTACTTT95600.021.34907314-15
CAGAGTA179950.019.7229310-11
TACTTTT110950.019.28032714-15
Produced by FastQC (version 0.11.5)