FastQCFastQC Report
Mon 15 Aug 2016
SRR2920996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694046
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57050.034.6321531
TCAACGC65950.029.0490724
ATCAACG66600.028.9454063
CAACGCA66600.028.4975595
GGTATCA42350.027.8233131
AACGCAG69550.027.2899286
GTACATG113100.027.098281
TATCAAC71450.026.8976962
TACATGG113000.025.8534092
ACATGGG116800.024.604423
ACGCAGA81250.023.1398647
CATGGGG80850.022.5181474
CGCAGAG86000.021.861798
GTGTAGC23150.020.8224141
AGAGTAC84450.018.00041410-11
GTATAGC17050.017.4518991
GCAGAGT98900.017.446129
GTATAAT18750.017.1391621
GTATAGG9200.016.818321
ATGGGGA49700.016.5201475
Produced by FastQC (version 0.11.5)