FastQCFastQC Report
Mon 15 Aug 2016
SRR2920996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694046
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55200.039.50561
GTATCAA82900.039.2423321
TCAACGC102300.031.2112794
ATCAACG103850.030.8593253
CAACGCA103400.030.8416125
AACGCAG106350.030.0379836
TATCAAC109900.029.0462072
GTACATG120550.026.6896691
TACATGG120100.025.835512
ACGCAGA124800.025.4553057
ACATGGG121550.025.0424373
CGCAGAG127600.024.8038148
CATGGGG82550.023.4524784
TATCGCG1300.002054810822.8811727
AGAGTAC126050.021.6890910-11
GCAGAGT144550.020.9898629
GAGTACT67550.020.17177412-13
CAGAGTA136200.018.9133459
AGTACTT75650.018.6411812-13
GTACTTT74250.018.63204214-15
Produced by FastQC (version 0.11.5)