FastQCFastQC Report
Mon 15 Aug 2016
SRR2920995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011937
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40180.1334025246876014No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38680.1284223408391344No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31300.10391983630467702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG93550.026.5834244
GTATCAA113250.024.8026241
GTACATG173100.024.5811821
TACATGG172250.023.9729752
GTATTAG7850.023.5009731
ACATGGG174250.023.253913
ATGGGGA49400.022.3997295
TATCAAC128000.021.4760172
TCAACGC129800.020.9469094
AACGCAG130500.020.925736
TACCGTA3151.0179065E-820.7759367
CAACGCA131950.020.739835
ATCAACG138650.019.8263973
GTATAGA9350.019.7307641
GTAGCGT3652.5956979E-919.5599086
TCTATAC8700.019.1496333
ACCGTAT3753.6307028E-919.0383118
CTAGTAC4406.730261E-1118.9320243
ACGCAGA143900.018.605027
CGCAGAG144200.018.3187648
Produced by FastQC (version 0.11.5)