FastQCFastQC Report
Mon 15 Aug 2016
SRR2920995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011937
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57870.19213549287385492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.15309085150187404No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38070.1263970660740912No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37880.12576624278661871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35270.11710072289028621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145900.034.0113221
TATCAAC177200.027.6896082
TCAACGC179950.027.0737634
CAACGCA180350.027.0202375
AACGCAG181900.026.85146
GGTATCA106000.026.4696881
ATCAACG185950.026.2962683
CATGGGG95300.024.4985494
ACGCAGA205050.023.5893577
CGCAGAG208400.023.1538318
AGTACTT103050.022.17456612-13
GAGTACT97500.021.88045912-13
GTACATG180300.021.7732641
TACATGG181300.021.2171972
GCAGAGT219650.021.0744069
ACATGGG179950.020.6528473
GTACTTT107100.020.0025314-15
TACTTTT114550.019.87047414-15
AGAGTAC201600.019.12662510-11
ATGGGGA53200.019.1251965
Produced by FastQC (version 0.11.5)