##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3011937
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.955208890491402	31.0	30.0	34.0	26.0	34.0
2	29.979169551023144	31.0	30.0	34.0	26.0	34.0
3	30.117699008976615	31.0	30.0	34.0	26.0	34.0
4	33.656017041525104	35.0	35.0	37.0	28.0	37.0
5	33.175751020024656	35.0	33.0	37.0	27.0	37.0
6	32.96691033046176	35.0	33.0	37.0	26.0	37.0
7	32.79872155360487	35.0	33.0	37.0	25.0	37.0
8	32.72324553933233	35.0	33.0	37.0	25.0	37.0
9	34.0319976812264	38.0	34.0	39.0	25.0	39.0
10-11	33.92939875568446	37.5	33.5	39.0	25.0	39.0
12-13	33.82586189551773	37.0	33.0	39.0	24.5	39.0
14-15	34.69928886294767	38.0	33.0	41.0	24.0	41.0
16-17	34.690759800088784	38.0	33.0	41.0	24.0	41.0
18-19	34.735675414193594	38.5	33.5	41.0	24.0	41.0
20-21	34.712889081013316	39.0	34.0	41.0	23.5	41.0
22-23	34.68897174808105	39.0	34.0	41.0	24.0	41.0
24-25	34.663621782261714	39.0	34.0	41.0	23.5	41.0
26-27	34.50906957881257	39.0	33.5	41.0	22.5	41.0
28-29	34.38460200196751	38.5	33.0	41.0	22.0	41.0
30-31	34.199910887910335	38.0	33.0	40.5	20.5	41.0
32-33	34.041857449209594	38.0	33.0	40.0	19.0	41.0
34-35	33.90445235076298	38.0	33.0	40.0	18.5	41.0
36-37	33.74718013690193	38.0	33.0	40.0	17.0	41.0
38-39	33.59422856454169	38.0	33.0	40.0	16.0	41.0
40-41	33.38727951481057	38.0	32.5	40.0	15.0	41.0
42-43	33.21559531291658	38.0	32.0	40.0	13.5	41.0
44-45	32.960503821959094	38.0	32.0	40.0	9.0	41.0
46-47	32.76135971635529	38.0	31.5	40.0	7.5	41.0
48-49	32.509621051170726	38.0	31.0	40.0	4.0	41.0
50-51	32.28958988850032	37.5	31.0	40.0	2.0	41.0
52-53	32.03217348171625	37.0	31.0	40.0	2.0	41.0
54-55	31.83341583837909	37.0	30.5	40.0	2.0	41.0
56-57	31.679256405429463	37.0	30.0	40.0	2.0	41.0
58-59	31.59443225406109	36.0	30.0	40.0	2.0	41.0
60-61	31.316526706899914	36.0	30.0	39.5	2.0	41.0
62-63	31.044936530876974	35.5	30.0	39.0	2.0	41.0
64-65	30.76240555496347	35.0	29.0	39.0	2.0	41.0
66-67	30.44425763221475	35.0	29.0	38.5	2.0	40.5
68-69	30.13398039202015	35.0	29.0	38.0	2.0	40.0
70-71	29.780657430749713	35.0	29.0	37.0	2.0	40.0
72-73	29.502406756847837	35.0	29.0	37.0	2.0	39.0
74-75	29.24075105156582	34.0	29.0	36.0	2.0	39.0
76-77	28.9220237674294	34.0	28.0	36.0	2.0	38.5
78-79	28.6185746913033	34.0	28.0	35.0	2.0	37.0
80-81	28.376130377229003	34.0	28.0	35.0	2.0	37.0
82-83	28.136787057631018	34.0	27.0	35.0	2.0	36.5
84-85	27.950643057939125	34.0	27.0	35.0	2.0	36.0
86-87	27.731785226583426	34.0	27.0	35.0	2.0	36.0
88-89	27.60958761753649	34.0	26.5	35.0	2.0	35.5
90-91	27.4288630539085	34.0	26.0	35.0	2.0	35.0
92-93	27.57312503548381	34.0	27.0	35.0	2.0	35.0
94-95	27.523138432178364	34.0	27.0	35.0	2.0	35.0
96-97	27.463336550532098	34.0	27.0	35.0	2.0	35.0
98-99	27.415616594902218	34.0	27.0	35.0	2.0	35.0
100-101	27.278694906301162	34.0	26.0	35.0	2.0	35.0
102-103	25.982331635754665	31.5	24.0	34.0	2.0	35.0
104-105	27.044122768836132	33.5	25.5	35.0	2.0	35.0
106-107	27.23615882403915	34.0	27.0	35.0	2.0	35.0
108-109	27.217543228825836	34.0	27.0	35.0	2.0	35.0
110-111	27.159226438003188	34.0	26.5	35.0	2.0	35.0
112-113	27.055360055671812	34.0	25.5	35.0	2.0	35.0
114-115	26.961564601118816	34.0	25.0	35.0	2.0	35.0
116-117	26.8351069428079	34.0	25.0	35.0	2.0	35.0
118-119	26.707040519107803	34.0	25.0	35.0	2.0	35.0
120-121	26.566584892047874	34.0	25.0	35.0	2.0	35.0
122-123	26.332547294315916	34.0	25.0	35.0	2.0	35.0
124-125	25.2339715272929	32.5	21.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106577.0
3	32725.0
4	16223.0
5	12813.0
6	14774.0
7	17659.0
8	19823.0
9	21219.0
10	22289.0
11	23625.0
12	24964.0
13	27149.0
14	30237.0
15	24998.0
16	18077.0
17	16404.0
18	14983.0
19	14169.0
20	14007.0
21	14686.0
22	16682.0
23	21538.0
24	28609.0
25	37829.0
26	46368.0
27	54372.0
28	63591.0
29	74662.0
30	87393.0
31	105228.0
32	126981.0
33	157880.0
34	202474.0
35	279194.0
36	452676.0
37	592810.0
38	175908.0
39	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01350796975198	18.912320271587753	13.511225084663565	24.562946673996702
2	16.16431452114939	20.40853525528971	38.075573444411575	25.35157677914932
3	19.418162983425415	24.45730848916277	29.19630524861879	26.928223278793027
4	13.48815888062346	16.337227740018605	34.7480890857995	35.42652429355844
5	13.954971173301905	37.502503095868825	33.84428037197582	14.698245358853454
6	33.86949185519371	35.4673427498906	16.23698726832407	14.426178126591626
7	28.572026229207736	32.167469022235196	20.478876615875716	18.781628132681355
8	26.972722116775426	35.272772191089956	18.927991276177774	18.826514415956837
9	26.615930561390634	15.577290638839855	18.86529580654964	38.94148299321987
10-11	25.789613161032964	25.815051076744467	26.825593864082492	21.569741898140077
12-13	26.397998487707895	23.01505019554528	27.456071633630803	23.130879683116028
14-15	23.61131430102954	24.32499663318226	24.885025872603602	27.178663193184597
16-17	23.326009370022902	27.42799348316734	25.761434766073275	23.48456238073648
18-19	23.456739478235335	26.07977954737923	27.92496745178222	22.53851352260322
20-21	24.22388929569467	25.19984126272513	27.7128548965302	22.863414545050002
22-23	23.91250851821004	24.94684836191334	27.615057272924115	23.525585846952506
24-25	23.52705102694144	25.36707079267499	27.703193202075404	23.40268497830817
26-27	23.33361837009339	25.38151616864103	27.768120658551144	23.516744802714435
28-29	23.43212754581893	25.433333001251945	27.863654024328284	23.27088542860084
30-31	23.66947795012486	25.432913353898083	27.547644338629944	23.349964357347112
32-33	23.388395966095647	25.570067784125612	27.478275106378668	23.563261143400073
34-35	23.472351700438757	25.609968326316324	27.65836260638847	23.259317366856447
36-37	23.78727490749202	25.273032008267137	27.502622505506142	23.437070578734698
38-39	23.81880424485024	25.35151368715942	27.32117933172088	23.508502736269456
40-41	23.658162290095376	25.46322705312084	27.411938410318776	23.466672246465006
42-43	23.854801409679574	25.298590420064187	27.29332820086506	23.55327996939118
44-45	23.79154920128522	25.50443898334553	27.042320084716227	23.661691730653022
46-47	23.86457905160495	25.58977616680991	26.930433591171234	23.61521119041391
48-49	23.912940228526754	25.78983483407183	26.559102393310063	23.738122544091354
50-51	23.677795465379965	26.100391819040276	26.454468575189754	23.767344140390005
52-53	23.956906751690727	25.905153338066118	26.28711395631723	23.850825953925927
54-55	23.872822290972316	26.076495273015922	26.194084502832204	23.85659793317956
56-57	23.77769707826242	26.03653629045582	26.196258232471394	23.989508398810365
58-59	23.662169509065553	25.77278809490361	26.456229182670416	24.10881321336042
60-61	23.68604008837073	25.903114400635907	26.576026100656403	23.83481941033696
62-63	23.51556587884413	25.91423003762493	26.68441830845269	23.88578577507825
64-65	23.518206735802156	25.95349456753449	26.573083132824475	23.95521556383888
66-67	23.61300415773657	26.035037255212952	26.405305826560856	23.946652760489627
68-69	23.83866221166759	25.723285573444965	26.396168448384	24.04188376650345
70-71	23.523977319283908	25.745930874733435	26.49444831298764	24.23564349299502
72-73	23.76258835451716	25.74674835613806	26.30873299111709	24.181930298227687
74-75	23.68876507538043	25.968767180881652	26.246583749548968	24.09588399418895
76-77	23.74154641109556	25.749885016247166	26.434191934035024	24.074376638622255
78-79	23.768586696956714	25.90556280701055	26.311290017237425	24.014560478795307
80-81	23.680822270376183	25.95211171718681	26.15684907054555	24.210216941891456
82-83	23.579034392413103	25.865905328525535	26.283155476665616	24.27190480239575
84-85	23.599547168708547	25.98687400291438	26.03017790464906	24.38340092372801
86-87	23.646058425628784	25.707768229196077	26.256326387454237	24.389846957720902
88-89	23.606270923518586	25.745142555183953	26.11774912220655	24.53083739909091
90-91	23.464923971311357	25.92804057874837	26.146940360313593	24.460095089626684
92-93	23.485339855964106	25.86993595268104	26.195851620113213	24.448872571241644
94-95	23.34228988955241	25.778592734728523	26.3244046729512	24.554712702767866
96-97	23.562967619347432	25.482691255918766	26.34511490846275	24.60922621627105
98-99	23.617812002207163	25.621644815050427	26.036077459284456	24.724465723457953
100-101	23.295466904803458	25.76508445514835	26.26987422930308	24.669574410745117
102-103	23.2638006124583	25.52826230525239	26.25051872637501	24.957418355914292
104-105	23.296926938020345	25.650833366267022	26.107958517499082	24.94428117821355
106-107	23.142751121320064	25.792744023569515	26.172801585911422	24.891703269199
108-109	23.12566415174539	25.719425774400932	26.21730713033314	24.937602943520535
110-111	23.04699129734211	25.840672169112285	26.1358030410734	24.976533492472203
112-113	22.998016806918013	26.03744075947413	25.989658175213325	24.974884258394535
114-115	23.014973671342982	26.057193331125095	25.85604454993064	25.071788447601286
116-117	22.902238883657976	25.934437187580784	26.089942199487727	25.073381729273514
118-119	22.94135430818212	25.941367091785107	25.9160323149561	25.20124628507667
120-121	22.89757282182	26.06522943824709	25.91234586716312	25.124851872769792
122-123	22.7955662223692	26.11983696973953	25.83882874003493	25.24576806785634
124-125	22.63138403382832	26.233343407218275	25.83446408399726	25.30080847495615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	150.0
1	130.0
2	239.0
3	517.5
4	467.0
5	274.0
6	406.5
7	898.0
8	1435.5
9	1549.0
10	1296.5
11	1058.5
12	1051.5
13	1172.5
14	1343.0
15	1535.0
16	1761.0
17	2034.0
18	2283.5
19	2486.0
20	2822.5
21	3303.5
22	4042.5
23	4860.0
24	5799.5
25	7181.5
26	9264.0
27	12528.0
28	15514.5
29	18154.5
30	21531.5
31	26594.0
32	31599.0
33	34799.5
34	37684.5
35	41705.5
36	46680.0
37	51619.5
38	58024.0
39	63579.0
40	69918.5
41	78289.0
42	85320.0
43	93169.0
44	101120.0
45	108026.0
46	114472.5
47	119605.5
48	124826.5
49	129061.0
50	135803.5
51	138615.5
52	132057.0
53	127641.5
54	125453.0
55	119632.5
56	110322.0
57	100732.0
58	93352.5
59	84407.0
60	71503.5
61	58879.0
62	47131.5
63	34743.0
64	24432.0
65	17720.0
66	12816.0
67	9176.0
68	6589.5
69	4798.0
70	3619.5
71	2606.0
72	1842.5
73	1511.0
74	1132.0
75	806.0
76	547.5
77	331.0
78	227.5
79	129.5
80	78.5
81	44.0
82	27.5
83	19.0
84	15.0
85	15.5
86	11.5
87	7.5
88	7.0
89	4.5
90	3.0
91	5.0
92	7.0
93	4.5
94	4.0
95	6.0
96	5.5
97	3.0
98	1.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049536228679417926
2	1.99207353938678E-4
3	0.0032205188886752947
4	0.12254572389794342
5	0.022112016287193257
6	0.0026228968268592603
7	0.00597622061816034
8	0.013712106196112336
9	0.030943542311807983
10-11	0.02270963834900929
12-13	0.02038555255305805
14-15	0.030993344150292652
16-17	0.04223195903499973
18-19	0.04659792020882243
20-21	0.022311223641131937
22-23	0.024602108211426735
24-25	0.022078815061536812
26-27	0.02090017155073297
28-29	0.02081716848659185
30-31	0.02647797746101595
32-33	0.02523293149889921
34-35	0.030545127603930626
36-37	0.03255380175614563
38-39	0.033018618915335876
40-41	0.035707918193508034
42-43	0.034529274682704184
44-45	0.031325356406857116
46-47	0.043975023381963164
48-49	0.03605653106290072
50-51	0.024087489213751815
52-53	0.03401465568502927
54-55	0.03426366487745262
56-57	0.025448739465666115
58-59	0.030677932506556413
60-61	0.04774336249396983
62-63	0.05640888239030232
64-65	0.04885560355346078
66-67	0.04264697435570531
68-69	0.03112614905291844
70-71	0.03936005301571713
72-73	0.04208255351954573
74-75	0.025813952947887027
76-77	0.021315186871438546
78-79	0.03476168326229931
80-81	0.025299333950212104
82-83	0.04226516026065618
84-85	0.021946010158911025
86-87	0.03768339112006659
88-89	0.03720197334804812
90-91	0.03979166894925093
92-93	0.029283481028985665
94-95	0.024402900857488054
96-97	0.01792866185448102
98-99	0.02764002035899157
100-101	0.025979959076169255
102-103	0.03175697234039092
104-105	0.027756224648789136
106-107	0.013927914162879237
108-109	0.019157107203769532
110-111	0.024253495342034047
112-113	0.012599865136621384
114-115	0.016085993830548248
116-117	0.015222761963480644
118-119	0.008848126637442949
120-121	0.005577805910282984
122-123	0.0034363268554421953
124-125	0.0831856708822263
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3011937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.34702657145452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.16408368316208	27.098710690119347
2	13.336141070705143	10.761472762808125
3	6.216792836474846	7.524873172874362
4	3.6586855025467115	5.904683247513903
5	2.3085241171717836	4.657104194818677
6	1.5048782244365475	3.6430417024886808
7	1.0448912270205046	2.951077787061321
8	0.7880303518984678	2.543574523772811
9	0.5926659202557621	2.1521076869299303
>10	3.158418458698982	22.310014695506087
>50	0.16107304781764215	4.43225886437255
>100	0.061995847307785866	4.234362877495761
>500	0.0027402285354240657	0.7196790725647421
>1k	9.964467400543342E-4	0.8731565563836455
>5k	8.303722833786118E-5	0.19388216529016025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5787	0.19213549287385492	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4611	0.15309085150187404	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3807	0.1263970660740912	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3788	0.12576624278661871	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3527	0.11710072289028621	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	8.300306414111583E-5	0.0
18-19	0.0	0.0	0.0	1.494055154540085E-4	0.0
20-21	0.0	0.0	0.0	2.98811030908017E-4	0.0
22-23	0.0	0.0	0.0	5.810214489878108E-4	0.0
24-25	0.0	0.0	0.0	0.0015438569930247546	0.0
26-27	0.0	0.0	0.0	0.002722500503828599	1.6600612828223166E-5
28-29	0.0	0.0	0.0	0.0056276077487676535	3.320122565644633E-5
30-31	0.0	0.0	0.0	0.015189560737824197	3.320122565644633E-5
32-33	0.0	0.0	0.0	0.03431346671593728	3.320122565644633E-5
34-35	0.0	0.0	0.0	0.058649965122112444	3.320122565644633E-5
36-37	0.0	0.0	0.0	0.09590174030864523	3.320122565644633E-5
38-39	0.0	0.0	0.0	0.153904281530457	6.640245131289266E-5
40-41	0.0	0.0	0.0	0.2235106511191967	6.640245131289266E-5
42-43	0.0	0.0	0.0	0.2861613639329109	6.640245131289266E-5
44-45	0.0	0.0	0.0	0.35940326773103154	1.1620428979756216E-4
46-47	0.0	0.0	0.0	0.4497106015165656	1.6600612828223166E-4
48-49	0.0	0.0	0.0	0.5442510915732965	1.6600612828223166E-4
50-51	0.0	0.0	0.0	0.6453156224715191	1.6600612828223166E-4
52-53	0.0	0.0	0.0	0.746097942951662	1.99207353938678E-4
54-55	0.0	0.0	0.0	0.8566580243876283	1.99207353938678E-4
56-57	0.0	0.0	0.0	0.9810630169223327	1.99207353938678E-4
58-59	0.0	0.0	0.0	1.1215706039004136	1.99207353938678E-4
60-61	0.0	0.0	0.0	1.2716069426418946	1.99207353938678E-4
62-63	0.0	0.0	0.0	1.4189373814923751	1.99207353938678E-4
64-65	0.0	0.0	0.0	1.5787348805768513	2.1580796676690117E-4
66-67	0.0	0.0	0.0	1.7609930088179135	2.3240857959512434E-4
68-69	0.0	0.0	0.0	1.9716049837695808	2.3240857959512434E-4
70-71	0.0	0.0	0.0	2.2064206522247973	2.3240857959512434E-4
72-73	0.0	0.0	0.0	2.4344134688076147	2.490091924233475E-4
74-75	0.0	0.0	0.0	2.6700591679042422	2.6560980525157065E-4
76-77	0.0	0.0	0.0	2.929078529863008	2.6560980525157065E-4
78-79	0.0	0.0	0.0	3.222261953022258	2.6560980525157065E-4
80-81	0.0	0.0	0.0	3.5385202280127377	2.8221041807979384E-4
82-83	0.0	0.0	0.0	3.8789655958939377	3.320122565644633E-4
84-85	0.0	0.0	0.0	4.214430779926672	3.320122565644633E-4
86-87	0.0	0.0	0.0	4.563176454221985	3.320122565644633E-4
88-89	0.0	0.0	0.0	4.952095611561596	3.320122565644633E-4
90-91	0.0	0.0	0.0	5.385969228440038	3.320122565644633E-4
92-93	0.0	0.0	0.0	5.830201627723289	3.6521348222090966E-4
94-95	0.0	0.0	0.0	6.2814394856200515	3.6521348222090966E-4
96-97	0.0	0.0	0.0	6.749311157570693	3.98414707877356E-4
98-99	1.6600612828223166E-5	0.0	0.0	7.256542882537051	4.3161593353380234E-4
100-101	3.320122565644633E-5	0.0	0.0	7.820299694183511	4.3161593353380234E-4
102-103	3.320122565644633E-5	0.0	0.0	8.408509208525942	4.3161593353380234E-4
104-105	3.320122565644633E-5	0.0	0.0	8.985131495114274	4.3161593353380234E-4
106-107	3.320122565644633E-5	0.0	0.0	9.565239910396532	4.3161593353380234E-4
108-109	3.320122565644633E-5	0.0	0.0	10.17132828475496	4.3161593353380234E-4
110-111	3.320122565644633E-5	0.0	0.0	10.827085692695432	4.98018384846695E-4
112-113	3.320122565644633E-5	0.0	0.0	11.516160530582146	5.312196105031413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	14590	0.0	34.011322	1
TATCAAC	17720	0.0	27.689608	2
TCAACGC	17995	0.0	27.073763	4
CAACGCA	18035	0.0	27.020237	5
AACGCAG	18190	0.0	26.8514	6
GGTATCA	10600	0.0	26.469688	1
ATCAACG	18595	0.0	26.296268	3
CATGGGG	9530	0.0	24.498549	4
ACGCAGA	20505	0.0	23.589357	7
CGCAGAG	20840	0.0	23.153831	8
AGTACTT	10305	0.0	22.174566	12-13
GAGTACT	9750	0.0	21.880459	12-13
GTACATG	18030	0.0	21.773264	1
TACATGG	18130	0.0	21.217197	2
GCAGAGT	21965	0.0	21.074406	9
ACATGGG	17995	0.0	20.652847	3
GTACTTT	10710	0.0	20.00253	14-15
TACTTTT	11455	0.0	19.870474	14-15
AGAGTAC	20160	0.0	19.126625	10-11
ATGGGGA	5320	0.0	19.125196	5
>>END_MODULE