FastQCFastQC Report
Mon 15 Aug 2016
SRR2920994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550267
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95250.039.239451
TCAACGC112000.032.353594
GGTATCA62400.032.33281
CAACGCA114850.031.3424745
ATCAACG116800.031.228793
TATCAAC120500.030.4677562
AACGCAG118300.030.0260686
GTACATG195650.028.867861
TACATGG195850.028.1946532
CATGGGG134600.027.98224
ACATGGG192550.027.6886673
ACGCAGA136800.025.5305947
CGCAGAG143050.024.2903568
GTATAGG11000.023.2650531
GCAGAGT156300.021.0511139
GTATCGC3401.0713848E-920.999616
GTATAAT30350.019.8057331
ATGGGGC67850.019.4675855
ATGGGGA66750.019.4318475
GTGTAGC33750.019.044881
Produced by FastQC (version 0.11.5)