FastQCFastQC Report
Mon 15 Aug 2016
SRR2920994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550267
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.1043894786833388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75850.046.4236341
GTATCAA123400.044.8546831
TCAACGC151300.036.1438564
CAACGCA155800.034.90475
ATCAACG159050.034.4201353
TATCAAC160450.034.1509172
AACGCAG162450.033.6160776
ACGCAGA189200.028.4560997
CGCAGAG197300.027.077988
GTACATG193350.025.5456851
TACATGG190350.025.4072362
ACATGGG185800.025.1362843
CATGGGG130750.024.6938234
GCAGAGT213000.024.3561529
AGAGTAC188700.022.43333810-11
GAGTACT100300.021.65338512-13
CAGAGTA200750.021.20532610-11
GTACCGT8850.020.8373496
GTACTTT105200.020.70137414-15
GTATAAT28900.020.4100741
Produced by FastQC (version 0.11.5)