##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920994_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4550267 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.037982826062734 31.0 30.0 34.0 26.0 34.0 2 30.13484922972652 31.0 30.0 34.0 26.0 34.0 3 30.245358788835908 31.0 30.0 34.0 26.0 34.0 4 33.80452707500461 35.0 35.0 37.0 30.0 37.0 5 33.300935087984946 35.0 33.0 37.0 27.0 37.0 6 33.074415633192515 35.0 33.0 37.0 26.0 37.0 7 32.92514307402181 35.0 33.0 37.0 26.0 37.0 8 32.85546078944378 35.0 33.0 37.0 26.0 37.0 9 34.196824494035184 38.0 34.0 39.0 26.0 39.0 10-11 34.087015333385935 38.0 33.5 39.0 25.0 39.0 12-13 33.98807971048732 38.0 33.5 39.0 25.0 39.0 14-15 34.89111485106258 39.0 34.0 41.0 25.0 41.0 16-17 34.874425830396326 39.0 34.0 41.0 25.0 41.0 18-19 34.92218324770832 39.0 34.0 41.0 25.0 41.0 20-21 34.88477961842679 39.0 34.0 41.0 24.0 41.0 22-23 34.841349090064384 39.0 34.0 41.0 24.0 41.0 24-25 34.80504330844761 39.0 34.0 41.0 24.0 41.0 26-27 34.65706232183738 39.0 34.0 41.0 23.5 41.0 28-29 34.541438667225464 39.0 33.5 41.0 23.0 41.0 30-31 34.37845196776365 38.0 33.0 41.0 22.5 41.0 32-33 34.24311804120505 38.0 33.0 41.0 21.5 41.0 34-35 34.12277532285468 38.0 33.0 40.0 20.5 41.0 36-37 33.98111275667999 38.0 33.0 40.0 19.0 41.0 38-39 33.83965050842072 38.0 33.0 40.0 18.0 41.0 40-41 33.64683314187937 38.0 33.0 40.0 16.5 41.0 42-43 33.49081867064065 38.0 33.0 40.0 15.0 41.0 44-45 33.26654633673145 38.0 32.0 40.0 14.5 41.0 46-47 33.1103948405665 38.0 32.0 40.0 11.0 41.0 48-49 32.896589364975725 38.0 32.0 40.0 9.0 41.0 50-51 32.68692672320108 38.0 31.5 40.0 8.0 41.0 52-53 32.43960255519072 37.0 31.0 40.0 7.0 41.0 54-55 32.23739409137969 37.0 31.0 40.0 5.5 41.0 56-57 32.08147422997376 37.0 31.0 40.0 2.0 41.0 58-59 31.99522863163854 36.5 31.0 40.0 2.0 41.0 60-61 31.72780179712531 36.0 31.0 39.5 2.0 41.0 62-63 31.456899342390237 35.5 30.0 39.0 2.0 41.0 64-65 31.17886818509771 35.0 30.0 39.0 2.0 41.0 66-67 30.860816738885873 35.0 30.0 39.0 2.0 40.5 68-69 30.539815355890106 35.0 29.5 38.0 2.0 40.0 70-71 30.18994687564488 35.0 29.0 37.0 2.0 40.0 72-73 29.917352542169503 35.0 29.0 37.0 2.0 39.0 74-75 29.66075221519968 34.5 29.0 36.0 2.0 39.0 76-77 29.350466906667236 34.0 29.0 36.0 2.0 39.0 78-79 29.04284836472233 34.0 29.0 35.5 2.0 37.5 80-81 28.793367839733364 34.0 29.0 35.0 2.0 37.0 82-83 28.5491678839945 34.0 28.5 35.0 2.0 36.5 84-85 28.362553230392855 34.0 28.0 35.0 2.0 36.0 86-87 28.14576595175624 34.0 27.5 35.0 2.0 36.0 88-89 28.02399738301071 34.0 27.0 35.0 2.0 35.5 90-91 27.843694556824907 34.0 27.0 35.0 2.0 35.0 92-93 27.99618439972863 34.0 28.0 35.0 2.0 35.0 94-95 27.944920704653157 34.0 27.5 35.0 2.0 35.0 96-97 27.885766153942175 34.0 27.5 35.0 2.0 35.0 98-99 27.83950436315056 34.0 27.5 35.0 2.0 35.0 100-101 27.70803119904832 34.0 27.0 35.0 2.0 35.0 102-103 26.39605807747106 31.5 24.5 34.0 2.0 35.0 104-105 27.469967146982803 33.5 27.0 35.0 2.0 35.0 106-107 27.670632294764243 34.0 27.0 35.0 2.0 35.0 108-109 27.65232710520064 34.0 27.0 35.0 2.0 35.0 110-111 27.590325249045826 34.0 27.0 35.0 2.0 35.0 112-113 27.484164775385707 34.0 27.0 35.0 2.0 35.0 114-115 27.394127970952034 34.0 27.0 35.0 2.0 35.0 116-117 27.268452928146854 34.0 27.0 35.0 2.0 35.0 118-119 27.13772785201396 34.0 27.0 35.0 2.0 35.0 120-121 26.995967379496633 34.0 27.0 35.0 2.0 35.0 122-123 26.763199280393877 34.0 25.5 35.0 2.0 35.0 124-125 25.634316733501574 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 149694.0 3 49011.0 4 24297.0 5 19132.0 6 21127.0 7 24795.0 8 27776.0 9 30094.0 10 31431.0 11 32413.0 12 33233.0 13 35621.0 14 37904.0 15 32198.0 16 24900.0 17 22705.0 18 21280.0 19 20152.0 20 20101.0 21 21430.0 22 24784.0 23 31671.0 24 42926.0 25 56421.0 26 69213.0 27 81931.0 28 95778.0 29 113471.0 30 132370.0 31 158511.0 32 192906.0 33 239559.0 34 306911.0 35 428529.0 36 695509.0 37 922425.0 38 277667.0 39 391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99260673336086 19.375794043961175 13.594978102087063 25.036621120590898 2 16.175130975945788 20.25293922571069 38.22058197515177 25.351347823191762 3 19.024626633598196 23.858461964482526 29.647979024890358 27.468932377028914 4 13.416147974873935 16.136245856058764 35.72617034362941 34.72143582543789 5 14.074087262493059 37.19863913100628 34.118395954144745 14.608877652355915 6 33.55039596531628 35.791598775072266 16.40426483018347 14.25374042942798 7 28.8441995031235 31.893436166490552 20.572814560978138 18.689549769407808 8 26.671809800747514 35.11923169489346 19.22167237576529 18.987286128593738 9 26.446582750853302 15.480328672833624 19.18267923447429 38.89040934183878 10-11 25.704238703412756 25.572307115722047 27.27416304937378 21.449291131491414 12-13 26.189790064653153 22.825949096497897 27.52346322652913 23.460797612319816 14-15 23.43354302376296 24.34309797824894 24.880763116057235 27.342595881930865 16-17 23.41315238479354 27.397509341316667 25.605821115228604 23.58351715866118 18-19 23.479197558963914 26.27606234416898 27.359312232343857 22.885427864523244 20-21 24.526813143357007 25.476698437220247 27.002977534567087 22.99351088485566 22-23 24.055698671204357 25.216715976168775 26.973920662663435 23.75366468996344 24-25 23.66267335746267 25.55602486528228 27.09780073988272 23.68350103737233 26-27 23.444339825140073 25.702325314551246 27.159088373734985 23.6942464865737 28-29 23.574807898975205 25.735690267885232 27.182660449445066 23.5068413836945 30-31 23.798250007611387 25.656208676628843 26.86849095937652 23.67705035638325 32-33 23.637658992358517 25.700014816058847 26.776597133774064 23.885729057808568 34-35 23.786698299226 25.704085130920127 26.93247015019787 23.576746419656004 36-37 23.975455966007768 25.458318274429935 26.853805967458698 23.712419792103596 38-39 23.831942737939542 25.67059322177353 26.753193335078862 23.744270705208066 40-41 23.817834482702523 25.696236106743214 26.841102132509736 23.64482727804453 42-43 24.040309886650427 25.482713970400724 26.629147284095005 23.84782885885384 44-45 23.976859003057115 25.57640078203582 26.5751155252708 23.871624689636267 46-47 24.019139786884754 25.705039825821256 26.405863912193016 23.869956475100977 48-49 23.990583849891195 25.76536722314915 26.27369027808662 23.970358648873034 50-51 23.83989142647937 25.98688487617152 26.2617148975677 23.911508799781412 52-53 24.099904888393624 25.752492406624818 26.19525105869781 23.952351646283752 54-55 24.000740420263245 25.859958124925253 26.108223149652833 24.031078305158672 56-57 23.983182191931537 26.01540544280509 26.00038051428341 24.001031850979967 58-59 23.789259013007317 25.689581055100863 26.343260885311842 24.177899046579977 60-61 23.871915482141233 25.8910804940835 26.36118601938443 23.875818004390833 62-63 23.682548987294137 25.894264864341316 26.302339773869022 24.120846374495528 64-65 23.668628905739418 25.878206328289714 26.245311287834404 24.207853478136457 66-67 23.727351714907087 26.043867957299028 26.10062333075664 24.128156997037244 68-69 23.94750379212557 25.679142210204663 26.16073116577634 24.212622831893423 70-71 23.726589915872534 25.557262561849054 26.39095241717514 24.32519510510327 72-73 23.864662320317922 25.619790012587902 26.20631294568405 24.309234721410125 74-75 23.666856923724726 25.91456106179925 26.074966116987536 24.343615897488487 76-77 23.717397913123527 25.63067575204642 26.23976020857774 24.41216612625231 78-79 23.78114617232672 25.793444436054052 26.090904004712396 24.334505386906834 80-81 23.793093632054813 25.832344955469967 25.951708687426507 24.42285272504872 82-83 23.801974547197762 25.74403876939319 26.08949054272732 24.364496140681734 84-85 23.740564953172623 25.76810260653266 25.96355189039453 24.527780549900182 86-87 23.587783322707587 25.667201618044714 26.09259788511003 24.652417174137668 88-89 23.583694256425883 25.663088323327294 25.971084822587642 24.78213259765918 90-91 23.454977628635344 25.7627061260957 26.067971047373828 24.71434519789512 92-93 23.559388578515833 25.70163679713766 26.1581211367934 24.580853487553107 94-95 23.40371606119188 25.618281989673562 26.189316645558552 24.788685303576003 96-97 23.470156413929477 25.384803236820304 26.2985165306305 24.84652381861972 98-99 23.380671585694614 25.573537621265185 26.068276160652193 24.977514632388008 100-101 23.32943732117326 25.53798600864875 26.225672089575596 24.906904580602387 102-103 23.261200076853036 25.3545329881102 26.212897416586102 25.171369518450664 104-105 23.25360958404011 25.558491786680214 26.04386203922113 25.144036590058548 106-107 23.13174463915449 25.584572484680194 26.025513752846273 25.258169123319036 108-109 23.10515755697122 25.48521761761216 26.161515293658375 25.24810953175824 110-111 23.101295734622575 25.579493163024807 25.97022927376345 25.348981828589164 112-113 22.9649800555473 25.726805397179024 26.075184915892173 25.2330296313815 114-115 22.96136923980138 25.81165523442811 25.93584304068019 25.291132485090316 116-117 22.860018148846255 25.800627989204767 25.98597393331568 25.353379928633295 118-119 22.90966599184722 25.736511834762354 25.866032331990947 25.487789841399483 120-121 22.76432770490348 25.845396347601184 25.890890893090894 25.499385054404446 122-123 22.757313700536923 25.88951585067802 25.750880727531104 25.60228972125395 124-125 22.670720069456745 26.011775583723978 25.748354981939247 25.569149364880033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 51.0 1 73.5 2 194.5 3 334.5 4 316.5 5 270.0 6 356.0 7 732.0 8 1117.0 9 1208.0 10 1020.5 11 783.5 12 761.0 13 832.5 14 924.5 15 1020.5 16 1195.0 17 1398.5 18 1604.5 19 1882.5 20 2185.5 21 2528.0 22 3057.5 23 3793.0 24 4716.0 25 6037.5 26 8487.0 27 12109.5 28 16115.0 29 22241.5 30 29448.5 31 38365.5 32 50241.0 33 60038.5 34 68785.5 35 77320.0 36 87261.5 37 96598.5 38 100933.0 39 104688.0 40 112906.5 41 121287.0 42 127106.0 43 136337.0 44 146380.0 45 155908.5 46 166331.5 47 174585.0 48 182449.5 49 189106.5 50 193234.5 51 194839.0 52 193235.5 53 193165.0 54 191534.5 55 182323.5 56 168667.0 57 152994.5 58 140664.5 59 128708.0 60 111334.5 61 93001.0 62 74703.5 63 55737.0 64 39952.0 65 29429.5 66 21240.0 67 15410.5 68 11446.0 69 8458.0 70 6605.0 71 5026.0 72 3831.5 73 3375.5 74 2640.5 75 1864.5 76 1333.0 77 838.0 78 484.0 79 279.5 80 164.0 81 101.5 82 68.0 83 39.5 84 25.0 85 21.0 86 14.5 87 10.0 88 8.0 89 8.0 90 7.5 91 7.0 92 5.0 93 2.0 94 3.0 95 8.5 96 10.0 97 5.5 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05153543737103779 2 8.790692941754847E-5 3 0.002461394023691357 4 0.13146481294394374 5 0.018042897262951822 6 0.002659184614880841 7 0.00534034596211607 8 0.011252086965446204 9 0.026020451107594343 10-11 0.020647140046946694 12-13 0.020086733371909825 14-15 0.02942684462252435 16-17 0.03937131601288452 18-19 0.0411074778688811 20-21 0.022712952888259082 22-23 0.024394172913369698 24-25 0.022690976155904697 26-27 0.02128446528522392 28-29 0.021273476919046727 30-31 0.025525974629620633 32-33 0.025251265475190795 34-35 0.03005318149462438 36-37 0.03215195943446835 38-39 0.032877191602163125 40-41 0.03543748092144922 42-43 0.033118935658061385 44-45 0.03011911169168754 46-47 0.04034928060265475 48-49 0.03266841264479645 50-51 0.023987603364813537 52-53 0.030778413662319155 54-55 0.03294312179922629 56-57 0.025503997897266248 58-59 0.029174112200448896 60-61 0.042338174880726775 62-63 0.05115084455483602 64-65 0.043437011498446136 66-67 0.039536141505542424 68-69 0.03004219312844719 70-71 0.03720660787597739 72-73 0.040689919954147745 74-75 0.02546004443255747 76-77 0.021537197707299373 78-79 0.03106411118292619 80-81 0.02519632364430483 82-83 0.0380307353392669 84-85 0.022086616016159053 86-87 0.034437539599324614 88-89 0.03327277278454209 90-91 0.03505288810524745 92-93 0.027712659498882155 94-95 0.023482138520662634 96-97 0.018987896754190468 98-99 0.02631713699437857 100-101 0.025734753586987312 102-103 0.02911917036956293 104-105 0.025547951361975022 106-107 0.01497714309951482 108-109 0.019009873486544857 110-111 0.02270196452208189 112-113 0.013867318115618272 114-115 0.015515573042197304 116-117 0.014647492114199015 118-119 0.008966506800589942 120-121 0.005955694468038909 122-123 0.004252497710573907 124-125 0.06541374385283324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4550267.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.69329747380636 #Duplication Level Percentage of deduplicated Percentage of total 1 69.5778702528409 30.400865825512803 2 14.201264996955127 12.410001920326284 3 5.845607743624169 7.6624163417207045 4 3.049560922916614 5.329814902779644 5 1.8213963858221236 3.979140705172092 6 1.1509954463819683 3.017447185785833 7 0.8229157591186781 2.516913214133891 8 0.5812783200284676 2.03183732416626 9 0.4213143733870621 1.6567752821752109 >10 2.2744243198844756 18.287346011089507 >50 0.1699568541192807 5.09390340744021 >100 0.07925760843867706 5.920888618681852 >500 0.0031938055540011653 0.9527133622900197 >1k 9.632109284059259E-4 0.7399358987255044 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4750 0.1043894786833388 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.1976732354387116E-5 6.593019706316135E-5 2 0.0 0.0 0.0 2.1976732354387116E-5 6.593019706316135E-5 3 0.0 0.0 0.0 2.1976732354387116E-5 6.593019706316135E-5 4 0.0 0.0 0.0 4.395346470877423E-5 6.593019706316135E-5 5 0.0 0.0 0.0 4.395346470877423E-5 6.593019706316135E-5 6 0.0 0.0 0.0 4.395346470877423E-5 8.790692941754847E-5 7 0.0 0.0 0.0 4.395346470877423E-5 8.790692941754847E-5 8 0.0 0.0 0.0 4.395346470877423E-5 8.790692941754847E-5 9 0.0 0.0 0.0 1.318603941263227E-4 8.790692941754847E-5 10-11 0.0 0.0 0.0 1.4284876030351627E-4 8.790692941754847E-5 12-13 0.0 0.0 0.0 1.6482549265790336E-4 1.7581385883509693E-4 14-15 0.0 0.0 0.0 1.7581385883509693E-4 2.1976732354387116E-4 16-17 0.0 0.0 0.0 2.1976732354387116E-4 2.856975206070325E-4 18-19 0.0 0.0 0.0 2.856975206070325E-4 3.5162771767019386E-4 20-21 0.0 0.0 0.0 3.845928162017745E-4 4.175579147333552E-4 22-23 1.0988366177193558E-5 0.0 0.0 5.164532103280972E-4 4.72499745619323E-4 24-25 2.1976732354387116E-5 0.0 0.0 7.911623647579362E-4 4.944764779737101E-4 26-27 2.1976732354387116E-5 0.0 0.0 0.0013955225045035819 5.494183088596779E-4 28-29 2.1976732354387116E-5 0.0 0.0 0.0034173818811071965 5.71395041214065E-4 30-31 4.395346470877423E-5 0.0 0.0 0.011680633246356752 5.823834073912585E-4 32-33 4.395346470877423E-5 0.0 0.0 0.029360914425461185 5.933717735684521E-4 34-35 4.395346470877423E-5 0.0 0.0 0.0523705532005045 5.933717735684521E-4 36-37 4.395346470877423E-5 0.0 0.0 0.09118146253835213 5.933717735684521E-4 38-39 4.395346470877423E-5 0.0 0.0 0.1525075341732694 6.263368721000328E-4 40-41 4.395346470877423E-5 0.0 0.0 0.2202288349233133 6.373252382772264E-4 42-43 4.395346470877423E-5 0.0 0.0 0.2887742631366467 6.593019706316134E-4 44-45 4.395346470877423E-5 0.0 0.0 0.3709562537758774 7.032554353403877E-4 46-47 4.395346470877423E-5 0.0 0.0 0.4680604456837368 7.47208900049162E-4 48-49 4.395346470877423E-5 0.0 0.0 0.5716807387346721 8.131390971123233E-4 50-51 4.395346470877423E-5 0.0 0.0 0.6781799837240321 8.790692941754847E-4 52-53 4.395346470877423E-5 0.0 0.0 0.7878658549047781 9.669762235930331E-4 54-55 4.395346470877423E-5 0.0 0.0 0.9118585788482303 0.0010768598853649687 56-57 4.395346470877423E-5 0.0 0.0 1.0574214655975132 0.0010878482515421623 58-59 4.395346470877423E-5 0.0 0.0 1.2153023108314303 0.001120813350073743 60-61 4.395346470877423E-5 0.0 0.0 1.3798530943349039 0.0011318017162509366 62-63 4.395346470877423E-5 0.0 0.0 1.549260735688697 0.0011867435471369042 64-65 4.395346470877423E-5 0.0 0.0 1.731535753835984 0.0011867435471369042 66-67 6.593019706316135E-5 0.0 0.0 1.9412267455953685 0.0011977319133140978 68-69 6.593019706316135E-5 0.0 0.0 2.1794325475845704 0.0012087202794912914 70-71 6.593019706316135E-5 0.0 0.0 2.4408238022076505 0.001241685378022872 72-73 6.593019706316135E-5 0.0 0.0 2.7021930800983767 0.0013295923074404204 74-75 6.593019706316135E-5 0.0 0.0 2.978968047369528 0.0013955225045035819 76-77 6.593019706316135E-5 0.0 0.0 3.291543111645976 0.001417499236857969 78-79 6.593019706316135E-5 0.0 0.0 3.6345010084023643 0.0015054061662755174 80-81 6.593019706316135E-5 0.0 0.0 4.002039880297135 0.0015823247295158724 82-83 6.593019706316135E-5 0.0 0.0 4.390665426885938 0.0017471502221737757 84-85 6.593019706316135E-5 0.0 0.0 4.789773874807786 0.0017581385883509693 86-87 6.593019706316135E-5 0.0 0.0 5.2202870732640525 0.0018350571515913243 88-89 6.593019706316135E-5 0.0 0.0 5.68900682091842 0.001933952447186066 90-91 6.593019706316135E-5 0.0 0.0 6.1947463742237545 0.001955929179540453 92-93 6.593019706316135E-5 0.0 0.0 6.711199584551851 0.002109766306021163 94-95 6.593019706316135E-5 0.0 0.0 7.2461022616914565 0.0021317430383755503 96-97 6.593019706316135E-5 0.0 0.0 7.810113120834448 0.0024723823898685506 98-99 6.593019706316135E-5 0.0 0.0 8.409935065348911 0.00259325441781768 100-101 6.593019706316135E-5 1.0988366177193558E-5 0.0 9.060039773490214 0.00259325441781768 102-103 6.593019706316135E-5 2.1976732354387116E-5 0.0 9.733373887730105 0.00259325441781768 104-105 6.593019706316135E-5 2.1976732354387116E-5 0.0 10.41432293973079 0.00259325441781768 106-107 6.593019706316135E-5 2.1976732354387116E-5 0.0 11.109688728155952 0.00259325441781768 108-109 6.593019706316135E-5 2.1976732354387116E-5 0.0 11.844019263045443 0.002615231150172067 110-111 6.593019706316135E-5 2.1976732354387116E-5 0.0 12.615061929332938 0.0026372078825264537 112-113 6.593019706316135E-5 2.1976732354387116E-5 0.0 13.410169117548486 0.0026372078825264537 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7585 0.0 46.423634 1 GTATCAA 12340 0.0 44.854683 1 TCAACGC 15130 0.0 36.143856 4 CAACGCA 15580 0.0 34.9047 5 ATCAACG 15905 0.0 34.420135 3 TATCAAC 16045 0.0 34.150917 2 AACGCAG 16245 0.0 33.616077 6 ACGCAGA 18920 0.0 28.456099 7 CGCAGAG 19730 0.0 27.07798 8 GTACATG 19335 0.0 25.545685 1 TACATGG 19035 0.0 25.407236 2 ACATGGG 18580 0.0 25.136284 3 CATGGGG 13075 0.0 24.693823 4 GCAGAGT 21300 0.0 24.356152 9 AGAGTAC 18870 0.0 22.433338 10-11 GAGTACT 10030 0.0 21.653385 12-13 CAGAGTA 20075 0.0 21.205326 10-11 GTACCGT 885 0.0 20.837349 6 GTACTTT 10520 0.0 20.701374 14-15 GTATAAT 2890 0.0 20.410074 1 >>END_MODULE