FastQCFastQC Report
Mon 15 Aug 2016
SRR2920993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089628
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31060.10052990198172725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93650.029.6107671
GTACATG148800.026.474431
CATGGGG85750.026.2271984
ACATGGG146850.025.2828463
TACATGG152750.025.28012
TCAACGC108250.025.0079274
AACGCAG108400.024.9717066
CAACGCA109400.024.5275085
GTATAGG7850.024.2578261
TATCAAC112600.024.0958232
ATCAACG113300.023.8944363
GTATAAT16600.023.301121
GGTATCA69600.021.8023091
ACGCAGA123650.021.6994427
CGCAGAG125150.021.2492088
GTATAGA8100.020.5704881
ATGGGGA46750.019.7262385
GCAGAGT134250.019.0998129
GTATAAG15250.016.7791581
TATACAG17100.016.0050095
Produced by FastQC (version 0.11.5)