##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920993_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3089628 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.931479776853394 31.0 28.0 33.0 16.0 34.0 2 28.03668402798007 31.0 28.0 34.0 16.0 34.0 3 28.06931546451547 31.0 28.0 34.0 16.0 34.0 4 31.45174532338521 35.0 32.0 37.0 16.0 37.0 5 31.5401012678549 35.0 32.0 37.0 17.0 37.0 6 31.465905280506263 35.0 32.0 37.0 17.0 37.0 7 31.352009368118104 35.0 32.0 37.0 15.0 37.0 8 31.301639226469984 35.0 32.0 37.0 13.0 37.0 9 32.42279653084449 37.0 32.0 39.0 11.0 39.0 10-11 32.365966550018314 37.0 32.0 39.0 11.0 39.0 12-13 32.28628122867866 37.0 32.0 39.0 10.0 39.0 14-15 33.13080733343949 38.0 32.0 40.0 10.0 41.0 16-17 32.988816129320426 38.0 32.0 40.0 10.0 41.0 18-19 33.09233344596825 38.0 32.0 40.0 10.0 41.0 20-21 33.104377938055976 38.0 32.0 40.0 10.0 41.0 22-23 33.05345093325151 38.0 32.0 40.0 10.0 41.0 24-25 32.96590900263721 38.0 31.5 40.0 9.5 41.0 26-27 32.80397785752848 38.0 31.0 40.0 8.5 41.0 28-29 32.71318051881974 38.0 31.0 40.0 8.0 41.0 30-31 32.56905329703123 38.0 31.0 40.0 7.0 41.0 32-33 32.45155517104324 38.0 30.5 40.0 2.0 41.0 34-35 32.25625123801312 38.0 30.0 40.0 2.0 41.0 36-37 32.09835973780662 38.0 30.0 40.0 2.0 41.0 38-39 31.94457811749505 38.0 30.0 40.0 2.0 41.0 40-41 31.843959531697667 38.0 30.0 40.0 2.0 41.0 42-43 31.620748355465448 37.0 30.0 40.0 2.0 41.0 44-45 31.444832840717396 37.0 30.0 40.0 2.0 41.0 46-47 31.230257008287083 37.0 29.5 40.0 2.0 41.0 48-49 31.041058664667723 37.0 29.0 40.0 2.0 41.0 50-51 30.807223879379656 36.5 29.0 40.0 2.0 41.0 52-53 30.62212716223442 36.0 28.5 40.0 2.0 41.0 54-55 30.393828803985464 36.0 28.0 40.0 2.0 41.0 56-57 30.182994198654335 35.5 28.0 39.5 2.0 41.0 58-59 29.920367273988973 35.0 28.0 39.0 2.0 41.0 60-61 29.719285946398728 35.0 27.5 39.0 2.0 41.0 62-63 29.58931366494607 35.0 28.0 39.0 2.0 41.0 64-65 29.35634047205683 35.0 27.0 39.0 2.0 40.0 66-67 29.09046752554029 35.0 27.0 38.0 2.0 40.0 68-69 28.782476563521563 34.0 26.0 37.5 2.0 40.0 70-71 28.454827085979282 34.0 26.0 37.0 2.0 39.5 72-73 28.17600436039549 34.0 26.0 36.5 2.0 39.0 74-75 27.877608728299975 34.0 26.0 36.0 2.0 39.0 76-77 27.603183943180213 34.0 26.0 36.0 2.0 38.5 78-79 27.289123642069534 34.0 25.5 35.0 2.0 37.0 80-81 26.98263803927204 33.0 25.0 35.0 2.0 37.0 82-83 26.726048087342555 33.0 24.5 35.0 2.0 36.5 84-85 26.485494208364244 33.0 24.0 35.0 2.0 36.0 86-87 26.416384917537 33.0 24.0 35.0 2.0 36.0 88-89 26.49988089180963 33.0 25.0 35.0 2.0 35.5 90-91 26.414837482052853 33.0 25.0 35.0 2.0 35.0 92-93 26.267549685593217 33.0 24.0 35.0 2.0 35.0 94-95 26.183943342046355 33.0 24.0 35.0 2.0 35.0 96-97 26.087674956337786 33.0 24.0 35.0 2.0 35.0 98-99 26.01734335007321 33.0 23.5 35.0 2.0 35.0 100-101 25.84205703728734 33.0 23.0 35.0 2.0 35.0 102-103 24.55401847730536 31.0 19.5 34.0 2.0 35.0 104-105 25.524280593003432 32.5 20.0 35.0 2.0 35.0 106-107 25.706104586053726 33.0 20.5 35.0 2.0 35.0 108-109 25.69382527605265 33.0 20.0 35.0 2.0 35.0 110-111 25.580880449037878 33.0 20.0 35.0 2.0 35.0 112-113 25.482192516380614 33.0 19.0 35.0 2.0 35.0 114-115 25.33815964252007 33.0 18.5 35.0 2.0 35.0 116-117 25.199415431242855 33.0 17.5 35.0 2.0 35.0 118-119 24.99950495658377 33.0 11.5 35.0 2.0 35.0 120-121 24.770299531205698 33.0 2.0 35.0 2.0 35.0 122-123 24.522970241077566 33.0 2.0 35.0 2.0 35.0 124-125 23.385630567822403 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 211640.0 3 29759.0 4 19353.0 5 18223.0 6 21593.0 7 22916.0 8 23032.0 9 23841.0 10 24655.0 11 25350.0 12 25630.0 13 25933.0 14 26220.0 15 23429.0 16 19235.0 17 17145.0 18 16036.0 19 15441.0 20 15901.0 21 16853.0 22 20430.0 23 27118.0 24 36537.0 25 45676.0 26 53183.0 27 59985.0 28 69424.0 29 79999.0 30 92711.0 31 110390.0 32 132707.0 33 163754.0 34 207813.0 35 281738.0 36 425440.0 37 511164.0 38 149209.0 39 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81838132849556 18.718388484139886 12.631654951592552 24.831575235772004 2 15.894409982123909 20.412412650912483 38.56496609628487 25.12821127067874 3 19.437137709604368 24.566708375757358 28.708307771002524 27.287846143635747 4 13.04425382114395 16.046166133202718 35.737897673984946 35.17168237166838 5 13.918052894350176 37.81008235628115 33.86921080598834 14.40265394338034 6 33.305939445806224 35.74426516678928 16.68462536134523 14.265170026059259 7 28.182269860510733 32.82495302615161 20.727564626466872 18.265212486870787 8 26.174544870740828 35.36957363312329 19.634744284746937 18.821137211388944 9 26.254901611726 16.619761162242337 19.04105620730561 38.08428101872606 10-11 25.087854448233283 26.66446025793943 26.939765041746437 21.307920252080848 12-13 25.405097200669907 24.153584762259893 27.086873685416695 23.354444351653502 14-15 22.926616197628714 25.49142254003899 24.4151172775591 27.16684398477319 16-17 23.1275177517072 28.397601342844066 25.35634500942205 23.11853589602669 18-19 23.19483497606103 27.448017600827285 26.866361721599123 22.49078570151256 20-21 24.041363434506387 26.607448732671905 26.547503602534007 22.8036842302877 22-23 23.69646640570533 26.297319201631797 26.31384236545678 23.692372027206094 24-25 23.42519076569582 26.505940839694347 26.57012414436421 23.498744250245622 26-27 23.173359930204253 26.626605210762023 26.528630934916368 23.671403924117353 28-29 23.232903883177126 26.79489968059887 26.585741788723986 23.38645464750001 30-31 23.49376639681925 26.757019671408997 26.26290808265381 23.486305849117947 32-33 23.131886146774352 26.82573009622197 26.238944053764417 23.803439703239256 34-35 23.332387624824218 26.88196667664165 26.4193517532319 23.36629394530223 36-37 23.678925424750908 26.557441575052778 26.24844648722013 23.51518651297619 38-39 23.451910323144038 26.70299736339738 26.274913564359192 23.570178749099394 40-41 23.46349783247974 26.744596088057627 26.472844864068556 23.31906121539408 42-43 23.429103731232654 26.66089342692218 26.25110776937164 23.658895072473527 44-45 23.47050044520485 26.7495773709707 26.196607380463877 23.58331480336058 46-47 23.463209139138776 26.812235697838645 26.197958019205785 23.526597143816797 48-49 23.39924697101251 26.87109415775415 26.101557764994425 23.62810110623891 50-51 23.310688219609805 27.04051227785545 26.063725272807964 23.585074229726786 52-53 23.610812840308554 26.829305396305468 25.860043517417893 23.699838245968085 54-55 23.395667880084893 26.84517087705636 25.890198759135025 23.86896248372372 56-57 23.402111556563735 26.912045556463077 25.845877635622415 23.839965251350776 58-59 23.350099275004386 26.565822856103356 26.01359552896713 24.070482339925125 60-61 23.319640109450848 26.639610358084493 26.264206504000832 23.776543028463824 62-63 23.2019131900998 26.694495062863137 26.323422805333458 23.780168941703607 64-65 23.248181361173433 26.624652303576813 26.15487975926929 23.97228657598047 66-67 23.393088215969463 26.777369529346956 25.929498818949376 23.900043435734208 68-69 23.68515387539685 26.39342689010976 26.140699625327297 23.78071960916609 70-71 23.35878223339426 26.406823977919757 26.222956834837426 24.011436953848552 72-73 23.519226473910976 26.432007328687085 26.101099626767837 23.94766657063411 74-75 23.555526209489482 26.534211255424704 26.027004422624785 23.883258112461032 76-77 23.464029895262684 26.333248802064613 26.315236682967125 23.88748461970558 78-79 23.579718919079877 26.430258073150693 26.22089497747045 23.769128030298983 80-81 23.614365875510764 26.36038722273723 26.16079104369397 23.864455858058033 82-83 23.564981084783156 26.333645781816017 26.18014575462782 23.92122737877301 84-85 23.639977835189356 26.441748430693863 26.035237129165047 23.883036604951737 86-87 23.56735764378056 26.27010584134416 26.16455907640673 23.997977438468553 88-89 23.53521200610559 26.148439229577153 26.13979741250403 24.176551351813227 90-91 23.324545816462273 26.399286641356028 26.198986279821725 24.07718126235997 92-93 23.432079095876947 26.25524631034592 26.265442748778533 24.0472318449986 94-95 23.363877025292457 26.187095582814717 26.348761304583622 24.1002660873092 96-97 23.492074281409337 25.987423399016173 26.405893572274188 24.114608747300302 98-99 23.265067862772955 26.29023584269774 26.245650501742873 24.199045792786432 100-101 23.2104617589903 26.245427844351017 26.258473804004367 24.285636592654317 102-103 23.117432757652413 26.147291451211164 26.439873955495997 24.295401835640426 104-105 23.000487637586062 26.409953179994268 26.261800692014862 24.327758490404804 106-107 22.937516489670788 26.458658068567896 26.257334362774376 24.34649107898694 108-109 23.033196403516595 26.467814425651415 26.214747019182855 24.28424215164914 110-111 22.960434839614045 26.69291070928518 26.10329383238021 24.243360618720573 112-113 22.913867565098027 26.74451122548295 26.194233885833228 24.147387323585797 114-115 22.951845996273434 26.826282252979116 26.090389269357615 24.131482481389835 116-117 22.968582240548503 26.68363527134932 26.174965551909075 24.172816936193104 118-119 23.12753927542124 26.52508655223042 26.02436862834459 24.32300554400375 120-121 23.0250926848595 26.6859079361416 26.02774711255528 24.261252266443623 122-123 23.082413387763186 26.650694634500983 25.9942962268704 24.27259575086543 124-125 23.123369707870758 26.694746809934557 25.927330484837483 24.254552997357205 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2230.0 1 1617.5 2 820.0 3 569.0 4 438.0 5 354.5 6 359.5 7 433.5 8 490.0 9 521.0 10 549.0 11 559.0 12 616.0 13 683.0 14 768.0 15 922.5 16 1060.0 17 1186.0 18 1374.0 19 1684.0 20 2109.5 21 2567.5 22 3278.0 23 4248.5 24 5377.5 25 7003.0 26 9633.0 27 13540.5 28 17298.0 29 21567.5 30 26656.5 31 32482.5 32 40196.0 33 46195.5 34 49885.0 35 53983.0 36 59853.5 37 66263.5 38 71330.5 39 73906.0 40 77850.0 41 84033.0 42 88381.0 43 94161.0 44 100306.5 45 106054.5 46 112309.0 47 116299.0 48 119083.0 49 122500.0 50 129249.0 51 131830.5 52 126974.0 53 123928.5 54 121487.0 55 114331.5 56 104736.0 57 96301.5 58 89485.5 59 80838.5 60 69232.0 61 59018.5 62 49647.5 63 37993.5 64 27235.5 65 20414.0 66 15287.5 67 11455.0 68 8506.5 69 6242.5 70 4935.0 71 3892.0 72 3009.5 73 2445.5 74 1920.0 75 1415.0 76 1017.0 77 700.5 78 486.5 79 318.5 80 205.5 81 148.0 82 97.5 83 70.0 84 51.5 85 32.5 86 23.0 87 21.0 88 22.0 89 19.5 90 14.5 91 13.5 92 12.5 93 12.5 94 10.5 95 7.5 96 7.0 97 8.0 98 8.0 99 9.5 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03744787398353459 2 0.018966684662360647 3 0.025019193249154914 4 0.02255935018714227 5 0.015827148122686614 6 0.004239992646363899 7 0.004628388919313263 8 0.005696478669924017 9 0.0026864075545664397 10-11 0.004628388919313263 12-13 0.007962123595461978 14-15 0.0024922094180917574 16-17 0.002136179501221506 18-19 1.6183178039556867E-5 20-21 0.0044018244267594675 22-23 8.41525258056957E-4 24-25 0.0012784710651249924 26-27 0.0013432037772832198 28-29 0.003107170183594918 30-31 0.0013755701333623336 32-33 3.236635607911373E-5 34-35 0.007557544144473056 36-37 0.008366703046450898 38-39 0.0014888523796392317 40-41 0.001213738352966765 42-43 0.0027835066228037806 44-45 0.0012622878870854354 46-47 0.023158127774605877 48-49 0.0034146505663464988 50-51 0.01187845268103474 52-53 0.0019581645427863806 54-55 2.912972047120236E-4 56-57 3.722130949098079E-4 58-59 0.007444261898196159 60-61 7.120598337405021E-4 62-63 0.010923645176700884 64-65 0.01187845268103474 66-67 1.9419813647468238E-4 68-69 0.004256175824403456 70-71 0.00546991417737022 72-73 0.012881809719487264 74-75 0.001941981364746824 76-77 0.0013432037772832198 78-79 6.311439435427177E-4 80-81 0.004531289851075922 82-83 0.001683050516113914 84-85 0.0014888523796392317 86-87 0.015406385493658137 88-89 0.0 90-91 5.825944094240472E-4 92-93 0.010163035808841711 94-95 0.007395712364077487 96-97 0.005194800150697754 98-99 0.0015374019137579023 100-101 0.01776912948743344 102-103 0.007865024527224635 104-105 0.007881207705264194 106-107 0.018545922033332166 108-109 0.010761813396305315 110-111 0.021782557641243542 112-113 0.0030748038275158047 114-115 0.014759058372075862 116-117 0.006376172147585405 118-119 0.0053080823969746515 120-121 0.008463802114688241 122-123 0.00872273296332115 124-125 0.05824325776436516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3089628.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.244812171782435 #Duplication Level Percentage of deduplicated Percentage of total 1 69.80297595782852 30.884195602855684 2 12.520091345396231 11.0789817990123 3 5.8392618945766 7.750711372421644 4 3.271896413302391 5.790577690083716 5 2.1293058321367355 4.710536829958538 6 1.4237945438696809 3.77973132988336 7 0.9848813493073291 3.0503123218116093 8 0.7304919936811949 2.585638484273228 9 0.5147111513001053 2.049596839079554 >10 2.6166209087369894 19.644764071404598 >50 0.11315561083749782 3.432052413811261 >100 0.049326146162897704 3.7271971333097405 >500 0.0023740274819029437 0.7237145761337737 >1k 0.0011128253817964473 0.7919895359610072 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3106 0.10052990198172725 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.473271215822747E-5 0.0 5 0.0 3.236635607911373E-5 0.0 6.473271215822747E-5 0.0 6 0.0 3.236635607911373E-5 0.0 6.473271215822747E-5 0.0 7 0.0 3.236635607911373E-5 0.0 6.473271215822747E-5 0.0 8 0.0 3.236635607911373E-5 0.0 6.473271215822747E-5 0.0 9 0.0 3.236635607911373E-5 0.0 6.473271215822747E-5 0.0 10-11 0.0 3.236635607911373E-5 0.0 1.6183178039556865E-4 0.0 12-13 0.0 3.236635607911373E-5 0.0 1.7801495843512553E-4 1.7801495843512553E-4 14-15 0.0 3.236635607911373E-5 0.0 2.2656449255379612E-4 2.1038131451423924E-4 16-17 0.0 3.236635607911373E-5 0.0 2.2656449255379612E-4 2.5893084863290986E-4 18-19 0.0 3.236635607911373E-5 0.0 2.5893084863290986E-4 3.722130949098079E-4 20-21 0.0 3.236635607911373E-5 0.0 4.693121631471491E-4 4.207626290284785E-4 22-23 0.0 3.236635607911373E-5 0.0 0.0010357233945316392 4.5312898510759225E-4 24-25 0.0 3.236635607911373E-5 0.0 0.0016668673380743572 4.5312898510759225E-4 26-27 0.0 3.236635607911373E-5 0.0 0.0029938879373180203 4.5312898510759225E-4 28-29 0.0 3.236635607911373E-5 0.0 0.007266246939761033 4.5312898510759225E-4 30-31 0.0 3.236635607911373E-5 0.0 0.020050957591010957 4.5312898510759225E-4 32-33 0.0 3.236635607911373E-5 0.0 0.04620297330293485 4.5312898510759225E-4 34-35 0.0 3.236635607911373E-5 0.0 0.08140138553897103 4.5312898510759225E-4 36-37 0.0 3.236635607911373E-5 0.0 0.13736281519975868 4.5312898510759225E-4 38-39 0.0 3.236635607911373E-5 0.0 0.2226319802901838 5.016785192262629E-4 40-41 0.0 3.236635607911373E-5 0.0 0.322433639260131 5.178616972658197E-4 42-43 0.0 3.236635607911373E-5 0.0 0.4145321054832491 5.178616972658197E-4 44-45 0.0 3.236635607911373E-5 0.0 0.5222311553365 5.664112313844903E-4 46-47 0.0 3.236635607911373E-5 0.0 0.6500620786709598 6.149607655031609E-4 48-49 0.0 3.236635607911373E-5 0.0 0.7799482656164431 6.796934776613884E-4 50-51 0.0 3.236635607911373E-5 0.0 0.9130872713478775 7.120598337405021E-4 52-53 0.0 3.236635607911373E-5 0.0 1.0484271892926915 7.444261898196158E-4 54-55 0.0 3.236635607911373E-5 0.0 1.1976846403515244 7.444261898196158E-4 56-57 0.0 3.236635607911373E-5 0.0 1.3717832697010772 7.444261898196158E-4 58-59 0.0 3.236635607911373E-5 0.0 1.5630037014164813 7.444261898196158E-4 60-61 0.0 3.236635607911373E-5 0.0 1.7594998491727805 7.444261898196158E-4 62-63 0.0 3.236635607911373E-5 0.0 1.9614173615723316 7.767925458987295E-4 64-65 0.0 3.236635607911373E-5 0.0 2.175553173391748 8.091589019778433E-4 66-67 0.0 3.236635607911373E-5 0.0 2.4222980889608716 8.41525258056957E-4 68-69 0.0 3.236635607911373E-5 0.0 2.6978976109745254 8.41525258056957E-4 70-71 0.0 3.236635607911373E-5 0.0 2.995829918682767 8.41525258056957E-4 72-73 0.0 3.236635607911373E-5 0.0 3.2955423759753604 8.738916141360707E-4 74-75 0.0 3.236635607911373E-5 0.0 3.6025210802077146 9.062579702151845E-4 76-77 0.0 3.236635607911373E-5 0.0 3.9492780360612993 9.062579702151845E-4 78-79 0.0 3.236635607911373E-5 0.0 4.332495692038005 9.062579702151845E-4 80-81 0.0 3.236635607911373E-5 0.0 4.733854690597056 9.062579702151845E-4 82-83 0.0 3.236635607911373E-5 0.0 5.166528138662648 9.386243262942982E-4 84-85 0.0 3.236635607911373E-5 0.0 5.60106265220279 9.386243262942982E-4 86-87 0.0 3.236635607911373E-5 0.0 6.06959802280404 0.0010357233945316395 88-89 0.0 3.236635607911373E-5 0.0 6.590049028556189 0.0011328224627689806 90-91 0.0 3.236635607911373E-5 0.0 7.144678906327881 0.0011328224627689806 92-93 0.0 3.236635607911373E-5 0.0 7.712190593819062 0.0011651888188480943 94-95 0.0 3.236635607911373E-5 0.0 8.285042730063296 0.0011651888188480943 96-97 0.0 3.236635607911373E-5 0.0 8.88775282979051 0.0013755701333623336 98-99 0.0 3.236635607911373E-5 0.0 9.537086665449692 0.0014888523796392317 100-101 0.0 3.236635607911373E-5 0.0 10.240342850336674 0.0014888523796392317 102-103 0.0 3.236635607911373E-5 0.0 10.95703107299649 0.0015050355576787886 104-105 0.0 3.236635607911373E-5 0.0 11.671227086238215 0.0015212187357183454 106-107 0.0 3.236635607911373E-5 0.0 12.391459424888692 0.0015212187357183454 108-109 0.0 3.236635607911373E-5 0.0 13.138426373660518 0.0015212187357183454 110-111 0.0 3.236635607911373E-5 0.0 13.936418882791067 0.001553585091797459 112-113 0.0 3.236635607911373E-5 0.0 14.76528889562109 0.0015859514478765728 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9365 0.0 29.610767 1 GTACATG 14880 0.0 26.47443 1 CATGGGG 8575 0.0 26.227198 4 ACATGGG 14685 0.0 25.282846 3 TACATGG 15275 0.0 25.2801 2 TCAACGC 10825 0.0 25.007927 4 AACGCAG 10840 0.0 24.971706 6 CAACGCA 10940 0.0 24.527508 5 GTATAGG 785 0.0 24.257826 1 TATCAAC 11260 0.0 24.095823 2 ATCAACG 11330 0.0 23.894436 3 GTATAAT 1660 0.0 23.30112 1 GGTATCA 6960 0.0 21.802309 1 ACGCAGA 12365 0.0 21.699442 7 CGCAGAG 12515 0.0 21.249208 8 GTATAGA 810 0.0 20.570488 1 ATGGGGA 4675 0.0 19.726238 5 GCAGAGT 13425 0.0 19.099812 9 GTATAAG 1525 0.0 16.779158 1 TATACAG 1710 0.0 16.005009 5 >>END_MODULE