FastQCFastQC Report
Mon 15 Aug 2016
SRR2920993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089628
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64000.2071446789063279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50350.16296460285833764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.1150623958612493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135150.036.4635851
GGTATCA93300.035.702621
TCAACGC166850.028.957364
ATCAACG170550.028.3282283
CAACGCA169600.028.2782735
TATCAAC171550.028.2626912
AACGCAG174950.027.4417536
CATGGGG93750.024.2112964
ACGCAGA198350.023.8456027
GTATTAC5600.023.4101721
CGCAGAG202300.023.322328
GTACATG166500.022.7979161
AGTACTT108350.022.13016512-13
TACATGG168800.021.9481432
GAGTACT103300.021.85848212-13
GCAGAGT213850.021.3949649
ACATGGG165050.021.331543
GTACTTT112350.019.99182114-15
AGAGTAC196800.019.78695510-11
TACTTTT127500.018.59630414-15
Produced by FastQC (version 0.11.5)