##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920993_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3089628 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.00570618857675 31.0 30.0 34.0 26.0 34.0 2 30.090902853029554 31.0 30.0 34.0 26.0 34.0 3 30.21105291640288 31.0 30.0 34.0 26.0 34.0 4 33.7341993922893 35.0 35.0 37.0 28.0 37.0 5 33.2615551127838 35.0 33.0 37.0 27.0 37.0 6 33.03234920191039 35.0 33.0 37.0 26.0 37.0 7 32.87353428956496 35.0 33.0 37.0 26.0 37.0 8 32.813967895164076 35.0 33.0 37.0 25.0 37.0 9 34.13777904653894 38.0 34.0 39.0 25.0 39.0 10-11 34.03809390645087 38.0 33.5 39.0 25.0 39.0 12-13 33.93105820506547 37.5 33.5 39.0 25.0 39.0 14-15 34.822058675024955 39.0 33.5 41.0 25.0 41.0 16-17 34.82316657539354 38.5 33.5 41.0 24.5 41.0 18-19 34.86700421539422 39.0 34.0 41.0 24.5 41.0 20-21 34.845798749881865 39.0 34.0 41.0 24.0 41.0 22-23 34.804034013156276 39.0 34.0 41.0 24.0 41.0 24-25 34.7614584992109 39.0 34.0 41.0 24.0 41.0 26-27 34.60187391491792 39.0 34.0 41.0 23.0 41.0 28-29 34.47423136377583 39.0 33.0 41.0 22.5 41.0 30-31 34.28097120430032 38.0 33.0 41.0 21.5 41.0 32-33 34.11128977339666 38.0 33.0 40.5 20.0 41.0 34-35 33.96026754677263 38.0 33.0 40.0 19.0 41.0 36-37 33.80016234964209 38.0 33.0 40.0 18.0 41.0 38-39 33.639261263815584 38.0 33.0 40.0 16.0 41.0 40-41 33.428108820867756 38.0 32.5 40.0 15.0 41.0 42-43 33.25525483974123 38.0 32.0 40.0 13.5 41.0 44-45 32.994785294540314 38.0 32.0 40.0 9.0 41.0 46-47 32.785480808692824 38.0 31.5 40.0 7.5 41.0 48-49 32.5289497635314 38.0 31.0 40.0 4.0 41.0 50-51 32.3062849313898 37.5 31.0 40.0 2.0 41.0 52-53 32.05622019867764 37.0 31.0 40.0 2.0 41.0 54-55 31.857879168624834 37.0 30.5 40.0 2.0 41.0 56-57 31.7054873596433 37.0 30.5 40.0 2.0 41.0 58-59 31.62353979184549 36.0 30.0 40.0 2.0 41.0 60-61 31.354783812161205 36.0 30.0 40.0 2.0 41.0 62-63 31.09185377657116 35.5 30.0 39.0 2.0 41.0 64-65 30.813250818545146 35.0 29.5 39.0 2.0 41.0 66-67 30.502366142461163 35.0 29.0 39.0 2.0 40.5 68-69 30.181664750578385 35.0 29.0 38.0 2.0 40.0 70-71 29.8299251560382 35.0 29.0 37.0 2.0 40.0 72-73 29.55551703959182 35.0 29.0 37.0 2.0 39.0 74-75 29.30000100335704 34.5 29.0 36.0 2.0 39.0 76-77 28.978256929313176 34.0 29.0 36.0 2.0 39.0 78-79 28.665354372759438 34.0 28.0 35.5 2.0 37.5 80-81 28.415864304699465 34.0 28.0 35.0 2.0 37.0 82-83 28.17328461549416 34.0 27.0 35.0 2.0 36.5 84-85 27.977005969650715 34.0 27.0 35.0 2.0 36.0 86-87 27.75445506708251 34.0 27.0 35.0 2.0 36.0 88-89 27.626295625233844 34.0 26.5 35.0 2.0 35.5 90-91 27.438727736801972 34.0 26.0 35.0 2.0 35.0 92-93 27.58101946253724 34.0 27.0 35.0 2.0 35.0 94-95 27.528313279139105 34.0 27.0 35.0 2.0 35.0 96-97 27.46417303312891 34.0 27.0 35.0 2.0 35.0 98-99 27.410701709073066 34.0 27.0 35.0 2.0 35.0 100-101 27.275853436077092 34.0 26.0 35.0 2.0 35.0 102-103 25.978704232354186 31.5 24.0 34.0 2.0 35.0 104-105 27.032888101739108 33.5 25.5 35.0 2.0 35.0 106-107 27.223431752948898 34.0 27.0 35.0 2.0 35.0 108-109 27.204207432092147 34.0 27.0 35.0 2.0 35.0 110-111 27.13498793382245 34.0 26.5 35.0 2.0 35.0 112-113 27.025622663958252 34.0 25.0 35.0 2.0 35.0 114-115 26.92599529781579 34.0 25.0 35.0 2.0 35.0 116-117 26.792970707153096 34.0 25.0 35.0 2.0 35.0 118-119 26.65364551978426 34.0 25.0 35.0 2.0 35.0 120-121 26.509640804653507 34.0 25.0 35.0 2.0 35.0 122-123 26.269381297683736 34.0 24.5 35.0 2.0 35.0 124-125 25.15549541886596 32.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104063.0 3 33252.0 4 16350.0 5 13471.0 6 15486.0 7 18609.0 8 20349.0 9 22107.0 10 23689.0 11 25028.0 12 26315.0 13 28991.0 14 32177.0 15 26969.0 16 19152.0 17 16504.0 18 15379.0 19 14961.0 20 14811.0 21 15343.0 22 17269.0 23 21998.0 24 29297.0 25 38547.0 26 46986.0 27 54476.0 28 64136.0 29 74813.0 30 88681.0 31 105962.0 32 128747.0 33 160226.0 34 203830.0 35 282840.0 36 456147.0 37 614114.0 38 198203.0 39 350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42217479865674 19.18118788475426 13.34833760253367 25.04829971405533 2 15.98071997780959 20.72019790116788 38.000526277801534 25.298555843221 3 19.118727081877477 24.72546804029479 29.540017562536192 26.615787315291545 4 13.365808634679436 16.494119347542654 35.24641831303654 34.89365370474137 5 13.640984071447349 37.58855490566013 34.19566053445662 14.574800488435905 6 33.1580540176628 36.08754687178856 16.31194884683667 14.44245026371197 7 27.921076405039187 32.46923662286302 20.98706929896367 18.62261767313412 8 26.491044185728835 35.05702626956849 19.58308705140813 18.86884249329455 9 26.448700823167044 15.5247892224882 19.38032933156609 38.64618062277867 10-11 25.430074736208958 25.94470763658636 27.17112474660068 21.454092880604 12-13 26.01989680929791 23.233948562626942 27.68036521402456 23.065789414050588 14-15 23.24169377564129 24.554871068335334 25.305137841365877 26.898297314657494 16-17 22.96006821479631 27.669375820691055 26.128164360488714 23.242391604023922 18-19 22.953667487976226 26.285597163932124 28.39464499044072 22.36609035765093 20-21 23.873609711193517 25.431377401830996 28.167336603785436 22.52767628319005 22-23 23.38963225887804 25.21618952516569 28.070556543052483 23.323621672903787 24-25 23.176833283370552 25.544405474194278 28.24378772702581 23.034973515409362 26-27 22.922494135235095 25.701906297461786 28.211085243324007 23.164514323979116 28-29 22.98038200064746 25.76961799935254 28.29962771123341 22.95037228876659 30-31 23.17443600965469 25.67806153757996 28.09456755978459 23.052934892980762 32-33 22.944684220741244 25.75098146590287 27.945000679049926 23.35933363430596 34-35 23.10618563226215 25.82193132392056 28.11449563763164 22.957387406185646 36-37 23.4272142160244 25.542354857660232 27.872214812556873 23.1582161137585 38-39 23.26278202231767 25.674982209193974 27.86098500521583 23.201250763272522 40-41 23.25134215144736 25.684253195901256 27.995750184266065 23.06865446838532 42-43 23.36975789232054 25.52725819908718 27.70942934282006 23.393554565772227 44-45 23.34833838286057 25.700156504047932 27.521466696106955 23.43003841698454 46-47 23.465916072682976 25.788479045045516 27.31017386519259 23.435431017078916 48-49 23.443982394920244 25.97718941649016 27.022790510040945 23.55603767854865 50-51 23.377159224358614 26.217255018125226 26.887520021107647 23.51806573640851 52-53 23.65980450354445 26.054371409594424 26.72599690387592 23.559827182985206 54-55 23.37202486320526 26.155367350992442 26.645142773056474 23.827465012745815 56-57 23.362462733026835 26.27070301965676 26.551615627896496 23.81521861941991 58-59 23.171001371750993 25.98207108176018 26.84147939314021 24.005448153348617 60-61 23.183566853035583 26.135188754788608 26.949013362933037 23.73223102924277 62-63 23.04327122277242 26.241500148069903 26.97652270343096 23.738705925726713 64-65 23.020604798358182 26.066081001902187 26.906498767681942 24.00681543205769 66-67 23.124148642798605 26.26334366321499 26.684784702752285 23.92772299123412 68-69 23.377110062463952 25.91804785217539 26.833582544864953 23.8712595404957 70-71 23.117724184834298 25.95951437715404 26.832600055333796 24.09016138267787 72-73 23.32056802001653 25.948020809808163 26.67735164674161 24.0540595234337 74-75 23.310526881483717 26.093191344987133 26.529011049916928 24.06727072361222 76-77 23.203796575228605 25.943668724563935 26.758416047681532 24.09411865252593 78-79 23.30579997426083 26.04214910750637 26.677955208985075 23.974095709247727 80-81 23.306076411539593 26.002637202687445 26.586085113725723 24.10520127204724 82-83 23.238710834697557 26.06064599436351 26.504493519074952 24.19614965186398 84-85 23.248257479754294 26.06931039076384 26.379946056775545 24.302486072706326 86-87 23.23198425167532 25.932074017773527 26.47742127658886 24.358520453962296 88-89 23.179697563422046 25.8634413585768 26.39184980838496 24.5650112696162 90-91 22.99134754250748 26.03537038239209 26.437258715229778 24.536023359870647 92-93 23.000585821753816 25.96150281814419 26.548910936477917 24.489000423624073 94-95 22.989386528082427 25.84519374940008 26.55642213870953 24.60899758380797 96-97 23.117304582733777 25.61986601253765 26.62093590517611 24.64189349955246 98-99 22.96115000076889 25.787201943754468 26.486843479042076 24.764804576434567 100-101 22.868090381290504 25.786095108876438 26.480502795258428 24.86531171457463 102-103 22.799486918991928 25.569308509381177 26.594337396087987 25.03686717553891 104-105 22.70517512903951 25.771335527980295 26.47960206929406 25.04388727368614 106-107 22.638341056488827 25.839110188900342 26.42154347653677 25.101005278074062 108-109 22.724656485040597 25.86573927501073 26.370644596765263 25.038959643183407 110-111 22.525586331165826 26.05127819967536 26.321774296412315 25.1013611727465 112-113 22.460053602293257 26.109664419992857 26.362736905449204 25.06754507226469 114-115 22.434596573257632 26.268260146000237 26.161386882508687 25.135756398233443 116-117 22.339197339387518 26.193479343528704 26.31124394607236 25.156079371011426 118-119 22.495698171544877 26.10238804887965 26.09792113897139 25.303992640604083 120-121 22.342995012714212 26.278946843716177 26.100695397506872 25.277362746062735 122-123 22.20671272582501 26.32420080239391 26.09115376087755 25.37793271090353 124-125 22.197556399318152 26.391622588294126 26.01463387933187 25.396187133055847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 88.0 1 98.5 2 251.5 3 480.0 4 413.0 5 279.5 6 435.5 7 894.0 8 1402.0 9 1496.5 10 1164.0 11 879.0 12 855.0 13 964.5 14 1117.5 15 1328.0 16 1541.0 17 1701.0 18 1936.0 19 2186.5 20 2566.5 21 3057.5 22 3771.5 23 4781.5 24 5856.5 25 7310.0 26 10045.0 27 14017.5 28 17692.5 29 22159.5 30 27423.5 31 33428.0 32 41417.0 33 47217.5 34 50620.5 35 54343.0 36 59863.0 37 66242.5 38 71110.5 39 73570.5 40 77481.0 41 84109.5 42 88349.5 43 93585.5 44 100310.0 45 106519.0 46 112750.0 47 116824.5 48 119967.5 49 123607.0 50 131612.0 51 133917.0 52 127229.5 53 123773.5 54 121344.0 55 114164.0 56 103767.0 57 95075.0 58 88762.0 59 80136.5 60 68426.0 61 58088.5 62 48184.5 63 36271.0 64 25528.0 65 18811.5 66 14105.5 67 10402.5 68 7551.0 69 5568.0 70 4337.5 71 3296.5 72 2493.0 73 2139.5 74 1711.0 75 1210.5 76 825.5 77 531.5 78 335.0 79 197.5 80 118.5 81 83.5 82 47.5 83 25.5 84 18.0 85 12.5 86 11.5 87 8.5 88 6.0 89 4.0 90 2.5 91 3.5 92 2.5 93 3.5 94 5.0 95 5.5 96 6.0 97 4.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052368764136006024 2 1.6183178039556867E-4 3 0.0031395365396740317 4 0.12949779067253403 5 0.01890195195020242 6 0.0026864075545664397 7 0.005146250616579083 8 0.011328224627689806 9 0.02673461012134794 10-11 0.020390804329841652 12-13 0.019096150086677102 14-15 0.027835066228037806 16-17 0.037172759956862124 18-19 0.04102435633027665 20-21 0.021248512765938164 22-23 0.023611256759713466 24-25 0.02142652772437329 26-27 0.020374621151802094 28-29 0.020326071617683423 30-31 0.024841178290719787 32-33 0.024388049305612197 34-35 0.028530942883738756 36-37 0.030570023316722917 38-39 0.0308775036994745 40-41 0.033126965446972904 42-43 0.031104068192028295 44-45 0.028806056910411222 46-47 0.039794434799270334 48-49 0.031524830821056775 50-51 0.022672632433419167 52-53 0.030019795263377985 54-55 0.03131444950654254 56-57 0.024824995112680236 58-59 0.028320561569224516 60-61 0.042367560107559876 62-63 0.05047533230537787 64-65 0.04345183303621018 66-67 0.03809520110511686 68-69 0.028692774664134325 70-71 0.035586808508985546 72-73 0.03820848335139376 74-75 0.023821638074227706 76-77 0.020504086576118546 78-79 0.030764221453197602 80-81 0.024242400703256187 82-83 0.038192300173354204 84-85 0.020698284712593232 86-87 0.033839025280713406 88-89 0.03280330188618177 90-91 0.03506894681171973 92-93 0.026038733465646995 94-95 0.02189583988752044 96-97 0.01725126779016762 98-99 0.024614613798165992 100-101 0.023837821252267265 102-103 0.027090640038218195 104-105 0.02422621752521663 106-107 0.014014632182256246 108-109 0.017283634146246733 110-111 0.021814923997322655 112-113 0.012768527473210368 114-115 0.014726692015996749 116-117 0.01341585459479264 118-119 0.00789739088330375 120-121 0.005567013245607561 122-123 0.003981061797730989 124-125 0.06910217022890781 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3089628.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.66757193444229 #Duplication Level Percentage of deduplicated Percentage of total 1 66.35412667008349 26.984612195027164 2 13.760072425960528 11.191774704117714 3 6.391591373932598 7.797915059248944 4 3.7372579159532555 6.079408205383717 5 2.383915848885958 4.847403463511338 6 1.6095335421652404 3.927349266414157 7 1.1101546474184913 3.160310578757284 8 0.833462368684279 2.7115912666494864 9 0.6118065203859965 2.2392617109982513 >10 3.006078077459406 20.859008592297105 >50 0.13612030307942055 3.7910788601996823 >100 0.06089901277532353 4.23003371995242 >500 0.0036154547536015238 1.0084745479301473 >1k 0.0012051515843381874 0.7981537746237756 >5k 1.606868779117583E-4 0.37362405488877004 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6400 0.2071446789063279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5035 0.16296460285833764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3555 0.1150623958612493 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.236635607911373E-5 0.0 3 0.0 0.0 0.0 3.236635607911373E-5 0.0 4 0.0 0.0 0.0 1.2946542431645493E-4 0.0 5 0.0 0.0 0.0 1.6183178039556865E-4 0.0 6 0.0 0.0 0.0 1.6183178039556865E-4 6.473271215822747E-5 7 0.0 0.0 0.0 1.9419813647468238E-4 6.473271215822747E-5 8 0.0 0.0 0.0 1.9419813647468238E-4 6.473271215822747E-5 9 0.0 0.0 0.0 1.9419813647468238E-4 6.473271215822747E-5 10-11 0.0 0.0 0.0 2.912972047120236E-4 6.473271215822747E-5 12-13 0.0 0.0 0.0 3.0748038275158043E-4 2.1038131451423924E-4 14-15 0.0 0.0 0.0 3.5602991687025106E-4 2.751140266724667E-4 16-17 0.0 0.0 0.0 3.5602991687025106E-4 3.236635607911373E-4 18-19 0.0 0.0 0.0 3.8839627294936477E-4 3.236635607911373E-4 20-21 0.0 0.0 0.0 6.311439435427177E-4 4.045794509889216E-4 22-23 0.0 0.0 0.0 0.0011651888188480943 4.207626290284785E-4 24-25 0.0 0.0 0.0 0.0018610654745490397 4.207626290284785E-4 26-27 0.0 0.0 0.0 0.0032204524298718165 4.5312898510759225E-4 28-29 0.0 0.0 0.0 0.007525177788393943 4.5312898510759225E-4 30-31 0.0 0.0 0.0 0.020309888439643868 4.5312898510759225E-4 32-33 0.0 0.0 0.0 0.04623533965901397 4.5312898510759225E-4 34-35 0.0 0.0 0.0 0.08109390515621945 4.8549534118670596E-4 36-37 0.0 0.0 0.0 0.1365212899417017 4.8549534118670596E-4 38-39 0.0 0.0 0.0 0.22104602884230723 5.340448753053766E-4 40-41 0.0 0.0 0.0 0.3195044840349712 5.502280533449334E-4 42-43 0.0 0.0 0.0 0.41058341004159726 5.825944094240472E-4 44-45 0.0 1.6183178039556867E-5 0.0 0.5176998654854241 6.149607655031609E-4 46-47 0.0 3.236635607911373E-5 0.0 0.6463723140779407 6.958766557009451E-4 48-49 0.0 3.236635607911373E-5 0.0 0.7756111739018419 8.577084360965139E-4 50-51 0.0 3.236635607911373E-5 0.0 0.9082161347579709 9.062579702151845E-4 52-53 0.0 3.236635607911373E-5 0.0 1.0419215517207898 0.0010680897506107532 54-55 0.0 3.236635607911373E-5 0.0 1.191680681298849 0.0011004561066898669 56-57 0.0 3.236635607911373E-5 0.0 1.3660706078531137 0.0011328224627689806 58-59 0.0 3.236635607911373E-5 0.0 1.5568217274053704 0.0011651888188480943 60-61 0.0 3.236635607911373E-5 0.0 1.753544439654224 0.001197555174927208 62-63 0.0 3.236635607911373E-5 0.0 1.9554619520537746 0.0012622878870854354 64-65 0.0 3.236635607911373E-5 0.0 2.169322649846519 0.0013593869553227768 66-67 0.0 3.236635607911373E-5 0.0 2.416261763552117 0.0013593869553227768 68-69 0.0 3.236635607911373E-5 0.0 2.6921040332363635 0.0013593869553227768 70-71 0.0 3.236635607911373E-5 0.0 2.9900687073006846 0.0013593869553227768 72-73 0.0 3.236635607911373E-5 0.0 3.290946353412126 0.0014241196674810042 74-75 0.0 3.236635607911373E-5 0.0 3.5991873455315657 0.0014888523796392317 76-77 0.0 3.236635607911373E-5 0.0 3.9461546826996647 0.0016345009819952435 78-79 0.0 3.236635607911373E-5 0.0 4.332026379874859 0.0016506841600348002 80-81 0.0 3.236635607911373E-5 0.0 4.736573464507702 0.0016506841600348002 82-83 0.0 3.236635607911373E-5 0.0 5.171674389279227 0.0017154168721930279 84-85 0.0 3.236635607911373E-5 0.0 5.606872413118991 0.0017154168721930279 86-87 0.0 3.236635607911373E-5 0.0 6.072931757480188 0.001812515940430369 88-89 0.0 3.236635607911373E-5 0.0 6.592703069754675 0.0019419813647468238 90-91 0.0 3.236635607911373E-5 0.0 7.149388211137392 0.0019743477208259376 92-93 0.0 3.236635607911373E-5 0.0 7.72101042585062 0.00216854585730062 94-95 0.0 3.236635607911373E-5 0.0 8.29515721633802 0.002184729035340177 96-97 0.0 3.236635607911373E-5 0.0 8.903434329310842 0.00242747670593353 98-99 0.0 3.236635607911373E-5 0.0 9.558836856734857 0.0025893084863290983 100-101 0.0 3.236635607911373E-5 0.0 10.270767225051042 0.0025893084863290983 102-103 0.0 3.236635607911373E-5 0.0 10.995563219908675 0.0026216748424082123 104-105 0.0 3.236635607911373E-5 0.0 11.715455711820322 0.002654041198487326 106-107 0.0 3.236635607911373E-5 0.0 12.444944828309428 0.002654041198487326 108-109 0.0 3.236635607911373E-5 0.0 13.203304734421103 0.002654041198487326 110-111 0.0 3.236635607911373E-5 0.0 14.015214776665669 0.0026864075545664397 112-113 0.0 3.236635607911373E-5 0.0 14.855218815986909 0.0026864075545664397 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13515 0.0 36.463585 1 GGTATCA 9330 0.0 35.70262 1 TCAACGC 16685 0.0 28.95736 4 ATCAACG 17055 0.0 28.328228 3 CAACGCA 16960 0.0 28.278273 5 TATCAAC 17155 0.0 28.262691 2 AACGCAG 17495 0.0 27.441753 6 CATGGGG 9375 0.0 24.211296 4 ACGCAGA 19835 0.0 23.845602 7 GTATTAC 560 0.0 23.410172 1 CGCAGAG 20230 0.0 23.32232 8 GTACATG 16650 0.0 22.797916 1 AGTACTT 10835 0.0 22.130165 12-13 TACATGG 16880 0.0 21.948143 2 GAGTACT 10330 0.0 21.858482 12-13 GCAGAGT 21385 0.0 21.394964 9 ACATGGG 16505 0.0 21.33154 3 GTACTTT 11235 0.0 19.991821 14-15 AGAGTAC 19680 0.0 19.786955 10-11 TACTTTT 12750 0.0 18.596304 14-15 >>END_MODULE