FastQCFastQC Report
Mon 15 Aug 2016
SRR2920992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3958888
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103750.032.009251
TCAACGC116900.027.8407864
GTACATG170500.027.8203961
CAACGCA118750.027.3061545
TACATGG169300.027.132232
ATCAACG121050.026.886993
AACGCAG121150.026.7635276
TATCAAC122650.026.6821162
CATGGGG102800.026.6818834
ACATGGG169700.025.8758223
GGTATCA77100.024.1612471
ACGCAGA137250.023.4506667
CGCAGAG140900.022.4206568
GTGTAGC29550.019.9391731
TATACCG4555.456968E-1219.6145135
GCAGAGT157650.019.2457799
GTATAGG10150.018.7634771
ATGGGGA57450.018.641475
GTATAGA16350.018.200431
AGAGTAC132100.018.0819310-11
Produced by FastQC (version 0.11.5)