FastQCFastQC Report
Mon 15 Aug 2016
SRR2920992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3958888
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50180.12675276491782542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42160.10649455099512792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134200.037.434711
GGTATCA93650.034.807991
TCAACGC159900.030.815454
CAACGCA162100.030.2792635
ATCAACG164450.029.962473
TATCAAC167250.029.7735582
AACGCAG167050.029.4821666
ACGCAGA192350.025.328927
CGCAGAG197500.024.6094478
GTACATG176000.023.532691
CATGGGG100550.023.5244754
TACATGG174300.022.9306162
ACATGGG173950.022.2978273
GAGTACT98800.021.8331312-13
GCAGAGT215150.021.7892729
AGAGTAC189550.020.45125210-11
AGTACTT109000.019.79002812-13
GTACTTT110000.019.52909914-15
GTATAAT24950.019.3470131
CAGAGTA203800.019.05046510-11
Produced by FastQC (version 0.11.5)