FastQCFastQC Report
Mon 15 Aug 2016
SRR2920991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6102956
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120900.043.567391
GGTATCA79050.039.301871
TCAACGC140800.036.892554
CAACGCA141900.036.5631335
ATCAACG149000.035.062753
AACGCAG150300.034.7147756
TATCAAC154750.033.567962
GTACATG248200.031.1255841
TACATGG256550.029.4095612
ACATGGG256200.028.9385453
ACGCAGA177450.028.8334227
CGCAGAG182950.027.7714928
CATGGGG192950.027.38394
GCAGAGT209850.022.8507169
ATGGGGA97100.020.5887225
AGAGTAC185200.020.06166510-11
CAGAGTA197200.019.09731310-11
GTATAGG16200.018.7369861
TAAGGTG29900.017.113415
ATGGGGG98600.017.0772865
Produced by FastQC (version 0.11.5)