FastQCFastQC Report
Mon 15 Aug 2016
SRR2920991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6102956
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62590.102556859331773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92650.052.2272831
GTATCAA154950.048.4964941
TCAACGC200750.037.0947954
CAACGCA203200.036.5131385
ATCAACG205850.036.2619863
TATCAAC207950.035.9194262
AACGCAG214350.034.8295866
ACGCAGA249450.029.6924447
CGCAGAG257450.028.6323058
GTACATG255100.027.4482251
TACATGG261200.026.1565862
ACATGGG258550.025.9214553
GCAGAGT280800.025.1500389
CATGGGG193150.024.705654
AGAGTAC256900.023.26413310-11
TAAGGTG28400.023.0452125
CAGAGTA271600.021.23826610-11
AGTACTT140850.019.87753712-13
GAGTACT130600.019.70619212-13
GTAAGGT25200.019.6216744
Produced by FastQC (version 0.11.5)