FastQCFastQC Report
Mon 15 Aug 2016
SRR2920990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004096
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73100.039.3211331
TCAACGC84900.033.4300044
CAACGCA85650.033.274555
ATCAACG89650.031.8578683
AACGCAG89700.031.5068686
GTACATG150150.031.4696161
TACATGG152300.030.8265252
TATCAAC93450.030.6912962
GGTATCA48850.030.0904481
ACATGGG156300.029.388953
CATGGGG115850.028.7619114
ACGCAGA101950.027.487667
CGCAGAG109550.025.580718
GCAGAGT124150.021.518099
ATGGGGG64800.020.2918365
AGAGTAC106250.020.13127710-11
ATGGGGA66700.019.2677945
CAGAGTA115350.017.51150910-11
TTACGTA4903.1468517E-1017.0003454
ACCGTAT4602.4901965E-916.8147188