##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920990_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4004096 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.69284802362381 31.0 30.0 33.0 22.0 34.0 2 28.794419014928714 31.0 30.0 34.0 20.0 34.0 3 28.838967397385076 31.0 29.0 34.0 22.0 34.0 4 32.296721657023205 35.0 33.0 37.0 22.0 37.0 5 32.33996162929161 35.0 33.0 37.0 24.0 37.0 6 32.383675865913304 35.0 33.0 37.0 23.0 37.0 7 32.31202998129915 35.0 33.0 37.0 23.0 37.0 8 32.336065618806344 35.0 33.0 37.0 23.0 37.0 9 33.56233192211176 37.0 33.0 39.0 22.0 39.0 10-11 33.4949280686577 37.0 33.0 39.0 21.0 39.0 12-13 33.431375646338154 37.0 33.0 39.0 20.5 39.0 14-15 34.35064131829007 38.0 33.0 41.0 19.0 41.0 16-17 34.204401318050316 38.0 33.0 41.0 18.0 41.0 18-19 34.30444861961351 38.5 33.0 41.0 19.0 41.0 20-21 34.315547129739144 39.0 33.0 41.0 19.0 41.0 22-23 34.264910981155296 39.0 33.0 41.0 19.0 41.0 24-25 34.18220667036954 39.0 33.0 41.0 18.5 41.0 26-27 34.03302218528227 38.0 33.0 41.0 18.0 41.0 28-29 33.95043750199795 38.0 33.0 41.0 18.0 41.0 30-31 33.829394450083115 38.0 33.0 40.0 17.0 41.0 32-33 33.749722159508664 38.0 33.0 40.0 16.5 41.0 34-35 33.578390478150375 38.0 32.0 40.0 16.0 41.0 36-37 33.43957787225881 38.0 32.0 40.0 15.0 41.0 38-39 33.3255603761748 38.0 32.0 40.0 15.0 41.0 40-41 33.24661658961223 38.0 32.0 40.0 13.0 41.0 42-43 33.04329229369126 38.0 32.0 40.0 9.0 41.0 44-45 32.891346136556166 38.0 31.5 40.0 9.0 41.0 46-47 32.70927582655361 38.0 31.0 40.0 8.0 41.0 48-49 32.54517948620612 38.0 31.0 40.0 7.5 41.0 50-51 32.3121383703088 37.0 31.0 40.0 6.5 41.0 52-53 32.130669444488845 37.0 31.0 40.0 2.0 41.0 54-55 31.90508244557573 37.0 30.0 40.0 2.0 41.0 56-57 31.698431306342307 36.0 30.0 40.0 2.0 41.0 58-59 31.425841188622847 36.0 30.0 39.5 2.0 41.0 60-61 31.22372965083754 36.0 29.5 39.0 2.0 41.0 62-63 31.0868811586935 35.0 30.0 39.0 2.0 41.0 64-65 30.840602473067577 35.0 29.0 39.0 2.0 41.0 66-67 30.56868678972732 35.0 29.0 38.5 2.0 40.0 68-69 30.252563499976024 35.0 29.0 38.0 2.0 40.0 70-71 29.917788559515056 35.0 29.0 37.0 2.0 40.0 72-73 29.631749588421457 34.0 29.0 37.0 2.0 39.0 74-75 29.31617710963973 34.0 29.0 36.0 2.0 39.0 76-77 29.03665009530241 34.0 29.0 36.0 2.0 38.5 78-79 28.721884290486543 34.0 28.0 35.0 2.0 37.0 80-81 28.41073016231379 34.0 28.0 35.0 2.0 37.0 82-83 28.15064361593728 34.0 27.0 35.0 2.0 36.5 84-85 27.91983458938047 34.0 27.0 35.0 2.0 36.0 86-87 27.86146560921616 34.0 27.0 35.0 2.0 36.0 88-89 27.97012221984688 34.0 28.0 35.0 2.0 35.5 90-91 27.89285384266511 34.0 28.0 35.0 2.0 35.0 92-93 27.74696847927722 34.0 27.0 35.0 2.0 35.0 94-95 27.66975267326258 34.0 27.0 35.0 2.0 35.0 96-97 27.57740760960776 34.0 27.0 35.0 2.0 35.0 98-99 27.515657341881912 34.0 27.0 35.0 2.0 35.0 100-101 27.34117675999776 34.0 26.5 35.0 2.0 35.0 102-103 26.00003121803273 31.5 24.5 34.0 2.0 35.0 104-105 27.01493208454543 33.5 25.5 35.0 2.0 35.0 106-107 27.20115326905249 34.0 26.0 35.0 2.0 35.0 108-109 27.189736334992965 34.0 26.5 35.0 2.0 35.0 110-111 27.083668823125123 34.0 25.5 35.0 2.0 35.0 112-113 26.988386017717858 34.0 25.0 35.0 2.0 35.0 114-115 26.854128996907168 34.0 25.0 35.0 2.0 35.0 116-117 26.71712204202896 34.0 25.0 35.0 2.0 35.0 118-119 26.520345915782237 34.0 25.0 35.0 2.0 35.0 120-121 26.300026897457002 34.0 24.5 35.0 2.0 35.0 122-123 26.067877118830317 34.0 24.0 35.0 2.0 35.0 124-125 24.89135200554632 32.5 19.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 197896.0 3 30069.0 4 19419.0 5 17486.0 6 19144.0 7 21361.0 8 23391.0 9 24565.0 10 26154.0 11 27007.0 12 27042.0 13 26932.0 14 26664.0 15 24355.0 16 21353.0 17 19229.0 18 18224.0 19 17888.0 20 18586.0 21 20259.0 22 24867.0 23 32786.0 24 43603.0 25 55333.0 26 65362.0 27 76031.0 28 88136.0 29 103468.0 30 119925.0 31 143510.0 32 174393.0 33 218089.0 34 282429.0 35 387799.0 36 608854.0 37 753877.0 38 198450.0 39 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97543310902893 18.717929820660572 12.966052046782895 25.340585023527606 2 16.140064015618734 19.64453696584751 38.98603136635428 25.229367652179473 3 19.29388418653496 23.71260362969987 28.518213947056303 28.475298236708866 4 13.3324024362858 15.677563574314934 35.28535283454404 35.70468115485522 5 14.875593159884282 36.83730821434334 33.527452331151444 14.759646294620934 6 34.583760727948246 34.4121035657386 16.896978978803475 14.107156727509679 7 29.889507729963288 31.253203126951224 20.48547666025625 18.37181248282924 8 26.837474359240126 34.98797041568938 19.42184067001026 18.75271455506023 9 26.899796252830306 15.890630876961984 18.745039313363545 38.46453355684416 10-11 25.853795354356247 25.54468435349871 27.080811053918286 21.520709238226765 12-13 26.333213031104165 22.81228731169475 27.157154257013016 23.697345400188073 14-15 23.474864691186713 24.257384323483404 24.361505488651442 27.906245496678437 16-17 24.102412779981677 27.263167542954413 25.000886610729438 23.633533066334465 18-19 24.052410837853394 26.582657681366488 26.154895656209998 23.21003582457012 20-21 24.8777144153007 25.829550191348687 25.754249494311487 23.538485899039124 22-23 24.553839906334975 25.51094440237599 25.70141376644083 24.2338019248482 24-25 24.24671234479549 25.732477379361274 25.83066591758831 24.190144358254923 26-27 24.06424237429306 25.98196078725192 25.786671262008138 24.167125576446878 28-29 24.099049739779524 26.103915613565754 25.865529506243558 23.931505140411165 30-31 24.302683025296805 25.77959005690123 25.706813624777052 24.210913293024912 32-33 24.12970970968471 25.837623732703648 25.667622771187997 24.365043786423648 34-35 24.383938678906574 25.897509704739885 25.599246224095978 24.119305392257562 36-37 24.40811652408364 25.725425110972495 25.652232281397147 24.214226083546723 38-39 24.304148126059395 25.908586533281685 25.67492305913339 24.112342281525525 40-41 24.40424524161042 25.744959584142045 25.73710505550679 24.113690118740745 42-43 24.343943138096456 25.688380654775024 25.6157785563571 24.35189765077142 44-45 24.234723726077565 25.807577083313298 25.62574916138255 24.331950029226583 46-47 24.213892092496714 25.879918037598493 25.571709732398208 24.334480137506592 48-49 24.292243840647036 25.945593797390202 25.51609743476011 24.246064927202653 50-51 24.14581381236921 25.958957801248516 25.61532639052019 24.279901995862087 52-53 24.40143230426788 25.80942361304059 25.466394857690517 24.322749225001015 54-55 24.189282387777602 25.651124605918717 25.6566439603091 24.50294904599458 56-57 24.122950237276047 25.936744952007217 25.619019944973786 24.321284865742953 58-59 24.229141511075078 25.716404399136177 25.592160908869417 24.462293180919325 60-61 24.32450488416391 25.70084436733388 25.784022379268258 24.190628369233952 62-63 24.20565991623033 25.73260369459226 25.752372826597487 24.309363562579918 64-65 24.087238010412086 25.65259727000945 25.719909778759142 24.540254940819317 66-67 24.128336115901348 25.909450200357387 25.50739929996666 24.45481438377461 68-69 24.153678377615957 25.688096994987085 25.68211541600258 24.47610921139437 70-71 24.00356602045554 25.660424340949316 25.729481957979498 24.60652768061564 72-73 24.27121052891211 25.68719609705412 25.59855181875005 24.44304155528372 74-75 24.090640270567672 25.813318894532657 25.5322018388492 24.563838996050475 76-77 24.142699909316885 25.671875472177813 25.643604118127307 24.54182050037799 78-79 24.208723024244136 25.752310486187657 25.60027811621716 24.43868837335104 80-81 24.11635788748083 25.890060790121733 25.573397703262284 24.420183619135152 82-83 24.26643133862264 25.736927755256172 25.592661541197227 24.403979364923966 84-85 24.287350121251627 25.681094486360127 25.49051340261284 24.5410419897754 86-87 24.187839158789416 25.629842014495335 25.732350692077194 24.449968134638052 88-89 24.027133715075763 25.560126430535128 25.76270648855572 24.650033365833387 90-91 24.00318325029935 25.676687311840524 25.806517271680274 24.51361216617985 92-93 24.18914708352456 25.539164723182257 25.80254198394635 24.469146209346835 94-95 24.06288740012905 25.553988038276614 25.803023061901975 24.580101499692358 96-97 24.10925573817904 25.46944537200603 25.808125372915143 24.613173516899785 98-99 24.00640101097512 25.47919546559675 25.690156603742953 24.82424691968517 100-101 23.8687461252441 25.523457353830842 25.89781934607858 24.709977174846472 102-103 23.917769974621564 25.432071538163935 25.965028661571665 24.685129825642836 104-105 23.91425509003318 25.55951745697463 25.745701708826513 24.78052574416568 106-107 23.847728078289123 25.62344866897453 25.80152155021573 24.72730170252062 108-109 23.856424679029868 25.623997407386824 25.722356855220163 24.797221058363146 110-111 23.668291561910255 25.830538788285267 25.829726965142815 24.671442684661663 112-113 23.815511888525112 25.85169482318499 25.772573687177307 24.56021960111259 114-115 23.71950574507223 26.030610495180355 25.65395835682804 24.595925402919374 116-117 23.707091621127507 25.939913284114407 25.73458745017333 24.618407644584753 118-119 23.82663446481065 25.928317569575736 25.52208965735993 24.72295830825368 120-121 23.747874051167134 26.00855112536202 25.69818351338934 24.545391310081506 122-123 23.729671433570267 26.008014412355102 25.64552010384169 24.616794050232944 124-125 23.75337582673786 26.121686567098912 25.405920714378976 24.719016891784253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 599.0 1 516.0 2 385.5 3 293.5 4 235.5 5 212.5 6 215.5 7 260.5 8 293.5 9 319.5 10 353.5 11 379.5 12 407.0 13 442.5 14 500.0 15 576.5 16 649.0 17 760.5 18 901.0 19 1060.5 20 1329.5 21 1680.5 22 2052.5 23 2609.5 24 3303.5 25 4296.5 26 5882.5 27 8122.0 28 10933.0 29 14511.0 30 19368.0 31 25953.0 32 32827.0 33 39615.5 34 46691.5 35 54171.0 36 62658.0 37 71696.5 38 81001.0 39 88736.0 40 96711.0 41 106342.5 42 116082.5 43 126755.0 44 135624.0 45 142734.0 46 150811.0 47 158695.0 48 168153.5 49 175312.5 50 177743.5 51 179228.0 52 177973.5 53 175370.0 54 172207.0 55 165271.0 56 153643.0 57 140174.5 58 128391.0 59 114811.0 60 97098.5 61 79550.5 62 65063.5 63 51334.0 64 38949.5 65 29867.5 66 22698.0 67 17609.5 68 13257.5 69 9613.5 70 7429.0 71 5863.5 72 4715.0 73 3655.5 74 2717.0 75 1993.0 76 1370.0 77 880.0 78 549.0 79 342.5 80 240.5 81 178.0 82 134.0 83 89.0 84 67.0 85 54.0 86 40.5 87 31.5 88 27.0 89 25.0 90 17.5 91 13.0 92 14.5 93 13.0 94 10.0 95 7.0 96 7.0 97 8.0 98 6.0 99 6.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03374044977942587 2 0.01743214947893357 3 0.022476983568825524 4 0.019704822262003707 5 0.013436241288920146 6 0.004620268844703024 7 0.004894987532766447 8 0.00541945048270571 9 0.002847084585384566 10-11 0.004857525893485071 12-13 0.00726755802058692 14-15 0.002672263602071479 16-17 0.0020978517997570487 18-19 1.2487213093791955E-5 20-21 0.0037211895019500034 22-23 9.989770475033566E-4 24-25 0.0016108504890991625 26-27 0.0010239514736909406 28-29 0.0021852622914135924 30-31 0.0013111573748481554 32-33 2.497442618758391E-5 34-35 0.006331017038552522 36-37 0.00720512195511796 38-39 0.001173798030816444 40-41 9.115665558468129E-4 42-43 0.001985466881912921 44-45 8.86592129659229E-4 46-47 0.01990461767150438 48-49 0.0025973403235087273 50-51 0.009677590147688767 52-53 0.001461003931973659 54-55 1.123849178441276E-4 56-57 1.123849178441276E-4 58-59 0.006605735726615945 60-61 8.241560641902692E-4 62-63 0.009477794738188095 64-65 0.009552718016750847 66-67 2.497442618758391E-5 68-69 0.003409009174605204 70-71 0.004820064254203695 72-73 0.011325902276069305 74-75 0.0017482098331308739 76-77 0.0010739003260661082 78-79 3.6212917971996676E-4 80-81 0.0038960104852630902 82-83 9.989770475033566E-4 84-85 6.493350808771818E-4 86-87 0.013298881944888435 88-89 0.0 90-91 3.121803273447989E-4 92-93 0.008328971133559236 94-95 0.006031323924301515 96-97 0.0042206780257016815 98-99 0.0010239514736909406 100-101 0.015434195383926859 102-103 0.007567251134837926 104-105 0.006655684578991113 106-107 0.016957635381369476 108-109 0.010326925228565948 110-111 0.01900553832875136 112-113 0.002722212454446647 114-115 0.013348830797263601 116-117 0.005394476056518126 118-119 0.004645243270890608 120-121 0.007379942938431047 122-123 0.007717097691963429 124-125 0.057403718592161625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4004096.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.32785171694714 #Duplication Level Percentage of deduplicated Percentage of total 1 74.55228950296964 39.011611502703104 2 13.114338039758215 13.724902726207738 3 4.799637769394317 7.5346420047577425 4 2.292125238305452 4.797679583468792 5 1.305377673385791 3.4153804663772562 6 0.8309945021629073 2.609049425206735 7 0.5789662931294116 2.1207243635190483 8 0.40720856573918846 1.7046679556696778 9 0.305357458184582 1.4380829813292024 >10 1.6696898979077723 15.665795932836282 >50 0.10011915078441216 3.5677283107897124 >100 0.04245011066032081 3.8760609831783444 >500 0.0013494111212514744 0.4766547545684139 >1k 9.63864967495012E-5 0.05701900938803725 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.994885237516783E-5 0.0 0.0 0.0 0.0 6 9.989770475033565E-5 0.0 0.0 0.0 0.0 7 1.748209833130874E-4 0.0 0.0 0.0 0.0 8 1.748209833130874E-4 0.0 0.0 0.0 0.0 9 1.748209833130874E-4 0.0 0.0 0.0 0.0 10-11 1.748209833130874E-4 0.0 0.0 0.0 0.0 12-13 1.8730819640687936E-4 0.0 0.0 0.0 1.4984655712550347E-4 14-15 2.2476983568825524E-4 0.0 0.0 0.0 1.748209833130874E-4 16-17 2.4974426187583914E-4 0.0 0.0 0.0 1.997954095006713E-4 18-19 3.1218032734479895E-4 0.0 0.0 1.2487213093791957E-5 1.997954095006713E-4 20-21 3.746163928137587E-4 0.0 0.0 8.741049165654369E-5 1.997954095006713E-4 22-23 3.995908190013426E-4 0.0 0.0 1.748209833130874E-4 1.997954095006713E-4 24-25 5.244629499392622E-4 0.0 0.0 3.746163928137587E-4 1.997954095006713E-4 26-27 5.244629499392622E-4 0.0 0.0 6.118734415958059E-4 1.997954095006713E-4 28-29 5.619245892206381E-4 0.0 0.0 0.0024724681925708076 1.997954095006713E-4 30-31 5.7441180231443E-4 0.0 0.0 0.0114882360462886 1.997954095006713E-4 32-33 5.7441180231443E-4 0.0 0.0 0.03255416453551563 2.122826225944633E-4 34-35 5.7441180231443E-4 0.0 0.0 0.05973882744070072 2.2476983568825524E-4 36-37 6.493350808771818E-4 0.0 0.0 0.1041683316284125 2.2476983568825524E-4 38-39 7.117711463461415E-4 0.0 0.0 0.17487093216546257 2.2476983568825524E-4 40-41 7.242583594399335E-4 0.0 0.0 0.2562126382584234 2.2476983568825524E-4 42-43 7.617199987213093E-4 0.0 0.0 0.3373295745156959 2.2476983568825524E-4 44-45 8.241560641902691E-4 0.0 0.0 0.4322323940285148 2.4974426187583914E-4 46-47 8.741049165654369E-4 0.0 0.0 0.5423696135157599 2.7471868806342305E-4 48-49 8.99079342753021E-4 0.0 0.0 0.6547045825075123 2.9969311425100695E-4 50-51 8.99079342753021E-4 0.0 0.0 0.7676514249408606 3.246675404385909E-4 52-53 8.99079342753021E-4 0.0 0.0 0.8873788240841378 3.496419666261748E-4 54-55 8.99079342753021E-4 2.4974426187583914E-5 0.0 1.0193436920593313 3.995908190013426E-4 56-57 9.240537689406049E-4 2.4974426187583914E-5 0.0 1.168753196726552 3.995908190013426E-4 58-59 9.240537689406049E-4 2.4974426187583914E-5 0.0 1.328901704654434 3.995908190013426E-4 60-61 9.240537689406049E-4 2.4974426187583914E-5 0.0 1.490523703727383 3.995908190013426E-4 62-63 9.240537689406049E-4 2.4974426187583914E-5 0.0 1.6608617775397994 4.245652451889265E-4 64-65 9.240537689406049E-4 2.4974426187583914E-5 0.0 1.8400657726488077 4.245652451889265E-4 66-67 9.240537689406049E-4 2.4974426187583914E-5 0.0 2.048202640496132 4.245652451889265E-4 68-69 9.240537689406049E-4 2.4974426187583914E-5 0.0 2.2716738060226325 4.245652451889265E-4 70-71 9.365409820343968E-4 2.4974426187583914E-5 0.0 2.511877837094815 4.245652451889265E-4 72-73 9.740026213157727E-4 2.4974426187583914E-5 0.0 2.756689649798606 4.495396713765105E-4 74-75 0.0010114642605971486 2.4974426187583914E-5 0.0 3.017435146410076 4.745140975640944E-4 76-77 0.0010239514736909406 2.4974426187583914E-5 0.0 3.309935625919059 4.745140975640944E-4 78-79 0.0010239514736909406 2.4974426187583914E-5 0.0 3.6290713309570997 4.745140975640944E-4 80-81 0.0011238491784412762 2.4974426187583914E-5 0.0 3.9619929192506875 4.8700131065788633E-4 82-83 0.00114882360462886 2.4974426187583914E-5 0.0 4.311372654401893 4.994885237516783E-4 84-85 0.0011987724570040278 2.4974426187583914E-5 0.0 4.679121080014066 4.994885237516783E-4 86-87 0.0012487213093791956 2.4974426187583914E-5 0.0 5.071419366568634 4.994885237516783E-4 88-89 0.0012487213093791956 2.4974426187583914E-5 0.0 5.506211639281376 4.994885237516783E-4 90-91 0.0012736957355667796 2.4974426187583914E-5 0.0 5.959047934914647 4.994885237516783E-4 92-93 0.0013236445879419474 2.4974426187583914E-5 0.0 6.42087502397545 4.994885237516783E-4 94-95 0.0013236445879419474 2.4974426187583914E-5 0.0 6.9009209569400936 4.994885237516783E-4 96-97 0.0013236445879419474 2.4974426187583914E-5 0.0 7.417154833450547 5.494373761268461E-4 98-99 0.001398567866504699 2.4974426187583914E-5 0.0 7.965880438431046 5.993862285020139E-4 100-101 0.001473491145067451 2.4974426187583914E-5 0.0 8.55008221581101 5.993862285020139E-4 102-103 0.0015234399974426189 2.4974426187583914E-5 0.0 9.14273783645547 6.243606546895978E-4 104-105 0.001798158685506042 2.4974426187583914E-5 0.0 9.748592441340069 6.243606546895978E-4 106-107 0.0019105436033501695 2.4974426187583914E-5 0.0 10.365710512435266 6.243606546895978E-4 108-109 0.0019480052426315453 2.4974426187583914E-5 0.0 11.019840683140464 6.243606546895978E-4 110-111 0.002047902947381881 4.994885237516783E-5 0.0 11.70525132264561 6.243606546895978E-4 112-113 0.0020853645866632567 4.994885237516783E-5 0.0 12.410666477527013 6.243606546895978E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7310 0.0 39.321133 1 TCAACGC 8490 0.0 33.430004 4 CAACGCA 8565 0.0 33.27455 5 ATCAACG 8965 0.0 31.857868 3 AACGCAG 8970 0.0 31.506868 6 GTACATG 15015 0.0 31.469616 1 TACATGG 15230 0.0 30.826525 2 TATCAAC 9345 0.0 30.691296 2 GGTATCA 4885 0.0 30.090448 1 ACATGGG 15630 0.0 29.38895 3 CATGGGG 11585 0.0 28.761911 4 ACGCAGA 10195 0.0 27.48766 7 CGCAGAG 10955 0.0 25.58071 8 GCAGAGT 12415 0.0 21.51809 9 ATGGGGG 6480 0.0 20.291836 5 AGAGTAC 10625 0.0 20.131277 10-11 ATGGGGA 6670 0.0 19.267794 5 CAGAGTA 11535 0.0 17.511509 10-11 TTACGTA 490 3.1468517E-10 17.000345 4 ACCGTAT 460 2.4901965E-9 16.814718 8 >>END_MODULE