FastQCFastQC Report
Mon 15 Aug 2016
SRR2920990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004096
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85150.048.158621
GGTATCA53050.042.4694481
TCAACGC113200.035.369594
ATCAACG115600.034.9436453
CAACGCA115450.034.6806835
TATCAAC118800.034.1987272
AACGCAG121050.033.1229556
ACGCAGA139650.028.3733547
GTACATG148950.027.8108651
CGCAGAG146150.026.9903418
TACATGG154950.026.181682
ACATGGG155050.025.7453823
AGAGTAC145750.023.63543910-11
CATGGGG118850.023.5115264
GCAGAGT165100.023.3518669
AGTACTT75850.020.94559912-13
CAGAGTA154350.020.237019
GAGTACT69150.018.8446912-13
GTACTTT73200.017.9646314-15
ATGGGGA69500.017.7195245
Produced by FastQC (version 0.11.5)