FastQCFastQC Report
Mon 15 Aug 2016
SRR2920989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443927
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51450.030.7716831
CGTACGA1055.9057836E-428.3347992
GGTATCA35050.028.0188881
GTACATG94800.027.5620271
TACCGTA2455.5479177E-1026.7123897
TCAACGC55250.026.7090764
TACATGG94050.026.6988832
CATGGGG61550.026.5854554
ACATGGG94400.026.0325973
CAACGCA57400.026.0180445
AACGCAG58650.025.5634026
ATCAACG57550.025.5382463
TATCAAC60450.024.7068212
ACCGTAT2704.5505658E-822.0354718
ACGCAGA69300.021.2055647
CGCAGAG71800.020.4672098
ATGGGGA34850.019.6339575
GTATAAG7700.019.3243241
GTACCGT3753.6288839E-919.0386476
GCAGAGT79550.018.024499
Produced by FastQC (version 0.11.5)