FastQCFastQC Report
Mon 15 Aug 2016
SRR2920989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443927
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37040.15155935508712004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.11154179318776705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.041.066521
GTATCAA72100.040.3349721
TATCAAC91250.031.7271842
TCAACGC90700.031.5963654
ATCAACG91200.031.3571663
CAACGCA91450.031.2285635
AACGCAG95850.029.8546436
ACGCAGA108700.026.3253697
CATGGGG60800.026.1641734
GTACATG96350.026.1628991
TACATGG95650.026.0327132
ACATGGG94300.025.8405673
CGCAGAG111550.025.4933058
GTATAGG5850.022.4111441
GCAGAGT123350.022.1863829
AGAGTAC110500.021.94139710-11
TACCGTA2255.085207E-621.1527277
AGTACTT70600.020.94404812-13
GAGTACT64600.020.54051212-13
ATGGGGA32450.019.9850985
Produced by FastQC (version 0.11.5)