FastQCFastQC Report
Mon 15 Aug 2016
SRR2920988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141673
Sequences flagged as poor quality0
Sequence length125
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG91550.027.6196634
GTATCAA87100.026.6467881
GTACATG148600.026.551751
TACATGG153450.025.1639862
ACATGGG149050.024.7887333
TCAACGC99450.022.3745654
TATCAAC102400.022.1955052
CAACGCA100050.022.1214525
AACGCAG100750.022.0271596
ATCAACG103450.021.6247983
ATGGGGA47750.019.437445
ACGCAGA114800.019.3831527
GTATAGA9400.018.9929221
CCGTGCG4105.3842086E-1018.8648879
CGCAGAG119450.018.4293618
GTATATA12550.018.0193161
ATGGGGC37550.017.9042575
GGTATCA67850.017.6296881
ATGGGGG47950.017.3710965
TCTATAC9450.016.9991283
Produced by FastQC (version 0.11.5)