FastQCFastQC Report
Mon 15 Aug 2016
SRR2920988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141673
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39360.12528356706761015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109000.031.8615951
GGTATCA81000.027.2843951
TATCAAC130150.026.3559782
TCAACGC130100.026.2795874
CAACGCA132400.025.9274465
ATCAACG135100.025.394763
CATGGGG91050.025.3825744
AACGCAG136850.025.0819596
GTACATG146800.025.0777111
ACATGGG143900.024.0901323
TACCGTA4000.023.7971537
TACATGG154000.023.395672
ACCGTAT4400.022.9873528
ACGCAGA151100.022.4820887
TATACCG3307.385097E-1021.6341195
CGCAGAG159300.021.251488
GTATAGG7300.021.2166081
CCCTATA5550.020.3873832
AGTACTT80450.019.45229312-13
GCAGAGT166750.019.1962329
Produced by FastQC (version 0.11.5)