FastQCFastQC Report
Mon 15 Aug 2016
SRR2920987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715295
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27930.10286175166970808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG63700.027.7414974
GTACATG128300.025.0027031
GTATCAA81700.024.9131661
TACATGG126850.024.8607542
GACGCGT1250.001634188723.7997768
ACATGGG129700.023.717643
AACGCAG90150.022.5721666
TCAACGC92200.021.9412254
CAACGCA92650.021.8342555
ATCAACG93250.021.4393353
TATCAAC96750.020.7256322
ACGCAGA105100.019.2481527
CGCAGAG106100.019.010668
TATACCG2601.7707447E-518.3071825
ATTATAC16550.017.6164723
TTACGCG850.00969540717.49983480-81
ATGGGGA37200.017.4336555
GGTATCA65200.016.7043021
GCAGAGT114600.016.4583959
CCGATCG2553.161349E-416.333189
Produced by FastQC (version 0.11.5)