FastQCFastQC Report
Mon 15 Aug 2016
SRR2920987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715295
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.14005844668811307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.10691287686973239No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27430.1010203311242425No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27390.10087301748060525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97950.031.016691
GGTATCA73600.027.680761
CATGGGG60200.025.3275454
ATCAACG119200.024.9325053
TCAACGC120050.024.557984
TATCAAC124250.024.2018472
CAACGCA121200.024.1030655
AACGCAG121550.023.9339986
GTACATG123400.023.89571
TACATGG124150.023.5018962
ACATGGG126850.022.2550983
GCGTATC2255.0279814E-621.180551
ACGCAGA136350.021.0754767
GAGTACT67800.021.01924912-13
CGCAGAG140200.020.4967258
AGTACTT74550.019.91426512-13
CGTATAG1500.004675477319.8567641
GTACCGT4254.1836756E-1119.597496
TACCGTA2851.8584469E-618.7881077
GTACTTT76200.018.70216614-15
Produced by FastQC (version 0.11.5)