FastQCFastQC Report
Mon 15 Aug 2016
SRR2920986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373859
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54400.036.6553041
GGTATCA39150.035.273381
TCAACGC64450.030.7443854
CAACGCA65000.030.3907745
AACGCAG65550.030.2259126
GTACATG90950.030.170961
TACATGG90650.029.3447192
ATCAACG67650.029.2919563
TATCAAC68550.028.995412
ACATGGG94100.027.255843
CATGGGG64400.026.8875734
ACGCAGA77800.025.084317
CGCAGAG79550.024.3081028
GCAGAGT87450.021.0235759
AGAGTAC74850.019.71409810-11
GTATAGT7600.019.580211
CAGAGTA82950.018.25527810-11
ATGGGGA36450.018.1193455
GAGTACT28900.018.11707712-13
ATGGGGG31650.018.0473965
Produced by FastQC (version 0.11.5)