FastQCFastQC Report
Mon 15 Aug 2016
SRR2920986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373859
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37010.15590647970245916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.1266713819144271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.103586607292177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70950.045.7751581
GGTATCA44350.045.0128941
CAACGCA91950.033.908295
TCAACGC93250.033.798064
TATCAAC94350.033.5892072
ATCAACG93950.033.5455323
AACGCAG95650.032.5268826
ACGCAGA110700.027.9990467
GTATACG1505.2344258E-627.8094621
CGCAGAG114100.027.1647198
GTACATG90650.026.623981
TACATGG91750.025.7110062
GTATAGG5800.025.6860771
ACATGGG93400.025.0681043
CATGGGG64650.024.3287814
GCAGAGT125950.023.6647459
AGAGTAC111850.023.40537310-11
AGTACTT69900.023.32333212-13
GAGTACT65900.021.44348512-13
CAGAGTA119050.020.16568610-11
Produced by FastQC (version 0.11.5)