FastQCFastQC Report
Mon 15 Aug 2016
SRR2920985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027290
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27380.1350571452530225No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25150.12405723897419708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG51100.029.5753124
GTACATG105550.027.4582561
TACATGG106400.026.2841642
ACATGGG104250.025.9123633
GTATCAA73500.025.0191971
TAGACGC2502.0116204E-823.7988015
TCAACGC79100.022.3406854
AACGCAG81000.022.1828336
CAACGCA81250.021.9681225
TATCAAC84450.021.4900862
ATCAACG83000.021.3631573
TATGCGT2357.3990086E-620.2552974
ACGCAGA90100.019.744297
CGCAGAG89800.019.4789758
ATGGGGG24800.019.432495
GGTATCA56600.018.9259781
ATGGGGA30800.018.544525
GTCTAGG7450.018.3727341
GTATATA11550.017.518591
TGTACGC1700.00969015317.5008472
Produced by FastQC (version 0.11.5)