##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920985_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2027290
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.553526135875973	31.0	29.0	33.0	16.0	34.0
2	28.673619955704414	31.0	29.0	34.0	16.0	34.0
3	28.802661188088532	31.0	29.0	34.0	18.0	34.0
4	32.20379965372492	35.0	33.0	37.0	22.0	37.0
5	32.348470618411774	35.0	33.0	37.0	23.0	37.0
6	32.31227698059972	35.0	33.0	37.0	22.0	37.0
7	32.13853370756034	35.0	32.0	37.0	19.0	37.0
8	32.209096873165656	35.0	33.0	37.0	21.0	37.0
9	33.36785610346818	37.0	33.0	39.0	18.0	39.0
10-11	33.32605522643529	37.0	33.0	39.0	18.0	39.0
12-13	33.28211035421671	37.0	33.0	39.0	18.0	39.0
14-15	34.21430653729856	38.0	33.0	41.0	17.0	41.0
16-17	34.093898504900636	38.0	33.0	41.0	17.0	41.0
18-19	34.203857612872355	38.5	33.0	41.0	17.5	41.0
20-21	34.23017476532711	39.0	33.0	41.0	18.0	41.0
22-23	34.204883119829915	39.0	33.0	41.0	18.0	41.0
24-25	34.14225715117226	39.0	33.0	41.0	18.0	41.0
26-27	34.00697285538823	38.5	33.0	41.0	18.0	41.0
28-29	33.93463589323679	38.0	33.0	41.0	17.0	41.0
30-31	33.81690310710357	38.0	33.0	40.5	16.5	41.0
32-33	33.71369118379709	38.0	33.0	40.0	16.0	41.0
34-35	33.54946899555564	38.0	32.0	40.0	15.5	41.0
36-37	33.410061214725076	38.0	32.0	40.0	14.5	41.0
38-39	33.29420260544865	38.0	32.0	40.0	10.5	41.0
40-41	33.21674748062685	38.0	32.0	40.0	9.0	41.0
42-43	33.00690873037405	38.0	32.0	40.0	9.0	41.0
44-45	32.85230578752916	38.0	31.5	40.0	8.0	41.0
46-47	32.66861598488623	38.0	31.0	40.0	7.5	41.0
48-49	32.507479443000264	38.0	31.0	40.0	3.5	41.0
50-51	32.296533796348825	37.5	31.0	40.0	2.0	41.0
52-53	32.126854816035205	37.0	31.0	40.0	2.0	41.0
54-55	31.924040467816642	37.0	30.0	40.0	2.0	41.0
56-57	31.72650829432395	37.0	30.0	40.0	2.0	41.0
58-59	31.475046490635282	36.0	30.0	40.0	2.0	41.0
60-61	31.29354927020801	36.0	29.5	40.0	2.0	41.0
62-63	31.18523990154344	36.0	30.0	39.0	2.0	41.0
64-65	30.96347981788496	35.5	29.5	39.0	2.0	41.0
66-67	30.70356535078849	35.0	29.0	39.0	2.0	41.0
68-69	30.40117348775952	35.0	29.0	39.0	2.0	40.0
70-71	30.074270084694344	35.0	29.0	38.0	2.0	40.0
72-73	29.781175362183014	35.0	29.0	37.0	2.0	40.0
74-75	29.470732110354216	34.5	29.0	37.0	2.0	39.0
76-77	29.17401062502158	34.0	29.0	36.0	2.0	39.0
78-79	28.839179643760882	34.0	28.0	36.0	2.0	38.5
80-81	28.51150748042954	34.0	28.0	35.5	2.0	37.0
82-83	28.227097504550407	34.0	27.5	35.0	2.0	37.0
84-85	27.965229937502777	34.0	27.0	35.0	2.0	36.5
86-87	27.869254522046674	34.0	27.0	35.0	2.0	36.0
88-89	27.926611880885318	34.0	28.0	35.0	2.0	36.0
90-91	27.81448534743426	34.0	27.0	35.0	2.0	36.0
92-93	27.646611239635178	34.0	27.0	35.0	2.0	35.0
94-95	27.56133187654455	34.0	27.0	35.0	2.0	35.0
96-97	27.454105234080963	34.0	27.0	35.0	2.0	35.0
98-99	27.378430811576045	34.0	27.0	35.0	2.0	35.0
100-101	27.19553147305023	34.0	26.0	35.0	2.0	35.0
102-103	25.861038874556673	31.5	24.0	34.0	2.0	35.0
104-105	26.855916272462252	33.5	25.0	35.0	2.0	35.0
106-107	27.031399553097977	34.0	25.0	35.0	2.0	35.0
108-109	27.02006447030272	34.0	25.0	35.0	2.0	35.0
110-111	26.90973393051808	34.0	25.0	35.0	2.0	35.0
112-113	26.806943752497176	34.0	25.0	35.0	2.0	35.0
114-115	26.666521563269193	34.0	25.0	35.0	2.0	35.0
116-117	26.530361961041585	34.0	25.0	35.0	2.0	35.0
118-119	26.342609345480913	34.0	24.5	35.0	2.0	35.0
120-121	26.128550429390963	34.0	24.0	35.0	2.0	35.0
122-123	25.898456066966247	34.0	23.5	35.0	2.0	35.0
124-125	24.753144592041593	32.5	11.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107002.0
3	15103.0
4	9527.0
5	8816.0
6	10062.0
7	11216.0
8	11681.0
9	12277.0
10	13129.0
11	13730.0
12	13949.0
13	14164.0
14	14622.0
15	13500.0
16	11479.0
17	10269.0
18	9670.0
19	9451.0
20	9429.0
21	10278.0
22	12192.0
23	16232.0
24	21734.0
25	27815.0
26	32052.0
27	36288.0
28	42034.0
29	49810.0
30	57944.0
31	69330.0
32	84812.0
33	106090.0
34	136528.0
35	187695.0
36	290040.0
37	393434.0
38	133706.0
39	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.147195347430326	19.526601232915176	12.924934088431787	24.401269331222718
2	15.351431142545902	20.56818450924362	39.89117435784089	24.189209990369587
3	19.000763762527086	25.186056103982224	29.28979952713824	26.523380606352454
4	12.460616227150505	16.521745565779412	37.02543355453043	33.992204652539655
5	13.334941623030709	38.323323456658564	34.72771063414962	13.614024286161111
6	32.39765527998568	36.83369072407018	17.27482287083667	13.493831125107475
7	27.9015941231139	32.79367165286435	21.510056255043914	17.79467796897784
8	25.81469929097816	36.4936346877576	19.87471135712591	17.816954664138336
9	26.0016120503228	16.677124275859892	19.516513156856245	37.804750516961064
10-11	24.623185110021808	26.976542394419067	27.958542592723223	20.4417299028359
12-13	25.17642775693113	24.39338968464625	27.956057519481604	22.47412503894101
14-15	22.507238309134934	25.73390004955026	25.156956434985688	26.601905206329114
16-17	22.58452935629954	28.85325093557027	26.048870890686572	22.513348817443624
18-19	22.68593114106298	27.947981775666474	27.589843483133514	21.77624360013703
20-21	23.556419699859212	27.026803710766966	27.332178363856823	22.084598225517
22-23	22.975585657865757	26.92868222139955	27.047881845130266	23.047850275604425
24-25	22.740559799650857	27.145961965430203	27.238253979137365	22.87522425578157
26-27	22.682183845819367	27.179464286374927	27.124439236535448	23.01391263127026
28-29	22.691436566901892	27.435868407870196	27.194947165290767	22.67774785993715
30-31	22.959756854618625	27.344729807087333	27.023607427644492	22.67190591064955
32-33	22.516202672633256	27.33791284814351	27.04214840607333	23.103736073149904
34-35	22.798567230503526	27.355962119713507	27.136167859572407	22.709302790210558
36-37	23.016679164117846	27.01440002367938	27.041581979990926	22.927338832211852
38-39	22.875261653008216	27.203329822017995	27.051005657648975	22.870402867324817
40-41	22.896288654149092	27.230915278850997	27.10076401284199	22.772032054157922
42-43	22.948156329396003	27.136651951108405	27.012467076209557	22.90272464328604
44-45	22.877477854323423	27.195823126607465	27.03765381613983	22.889045202929275
46-47	22.939219742716695	27.26790550391678	26.96067136604478	22.832203387321744
48-49	22.778699269808346	27.378450405298913	26.894508825564227	22.94834149932851
50-51	22.699382719855947	27.537946387277003	26.865314529208266	22.897356363658787
52-53	22.9077927773056	27.398213046350005	26.62665020429163	23.067343972052768
54-55	22.7988686357679	27.416567067218406	26.712374715568142	23.07218958144555
56-57	22.797371061352095	27.332630586174	26.713007859784675	23.156990492689225
58-59	22.78316502336081	27.13260188257882	26.874032182715023	23.210200911345346
60-61	22.75429400409269	27.318106156813005	26.97533067179605	22.95226916729826
62-63	22.597092246112968	27.19433890544706	27.066146331933666	23.142422516506304
64-65	22.5226174111084	27.307442131568127	26.843241613915115	23.326698843408362
66-67	22.649854066193818	27.333720056686538	26.723866207531337	23.292559669588304
68-69	22.983248482689948	27.023472645255907	26.840337324162345	23.152941547891796
70-71	22.605374663414494	27.102430765808016	26.909870683584593	23.382323887192893
72-73	22.74100769113127	26.914889418404446	26.936990937390544	23.407111953073738
74-75	22.688861944863184	27.11962226624468	26.825455001065485	23.36606078782665
76-77	22.639235244195184	26.961124650174632	26.96963366590842	23.430006439721762
78-79	22.725957707298434	27.009389197773466	27.023866749306276	23.24078634562182
80-81	22.745943994092283	27.042308547987133	26.889386679715997	23.32236077820459
82-83	22.61653042706386	26.934129222870695	27.009748923356497	23.439591426708947
84-85	22.64963546856348	27.10710515671399	26.803222083008592	23.44003729171394
86-87	22.604351389999266	26.881862334336393	27.019482116458555	23.494304159205786
88-89	22.619013559974153	26.79039505941429	26.88887628311687	23.701715097494684
90-91	22.51036489869406	26.977593074447228	26.903404816810745	23.60863721004797
92-93	22.598973738145116	26.859492206837913	27.046141330904206	23.49539272411276
94-95	22.367672645777517	26.79808673714785	27.186323604061542	23.647917013013085
96-97	22.53622357303972	26.57733019101776	27.220419624919995	23.666026611022524
98-99	22.574687650988935	26.727535374726326	27.00396767162763	23.693809302657108
100-101	22.32144531045718	26.730685169671727	27.077912531069398	23.869956988801697
102-103	22.27737962279639	26.59573051134413	27.244727515973338	23.882162349886144
104-105	22.246399886341408	26.73986069423003	27.02857028691148	23.985169132517086
106-107	22.14159782346192	26.806284608156787	27.079609953877583	23.97250761450371
108-109	22.145143510864045	26.850195342898996	27.086002920955377	23.91865822528158
110-111	22.088605159235293	26.999272749519935	26.981140826085497	23.930981265159275
112-113	22.057109370259855	27.023731361910546	26.931043446905218	23.98811582092438
114-115	22.03174886657883	27.09347126220159	26.92400386099529	23.95077601022429
116-117	21.979423826696635	26.993851714512562	27.04614186267867	23.98058259611213
118-119	21.97954930732201	26.85395910601413	26.927608632434847	24.238882954229016
120-121	21.853799721374813	27.054120771783875	26.95646876122295	24.135610745618365
122-123	21.84578788026783	27.012181495552	26.94444896652395	24.197581657656215
124-125	21.75630906360701	27.09502369639008	26.899431311315414	24.2492359286875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	921.5
2	423.5
3	294.5
4	230.5
5	177.0
6	167.0
7	204.0
8	281.0
9	316.5
10	318.0
11	336.5
12	382.0
13	453.5
14	522.0
15	579.0
16	662.5
17	762.5
18	901.0
19	1099.5
20	1364.5
21	1764.0
22	2370.0
23	3167.0
24	4150.5
25	5601.5
26	7634.0
27	10241.0
28	13086.5
29	16305.5
30	20212.0
31	25345.0
32	30791.0
33	34773.5
34	37880.5
35	41015.0
36	45142.0
37	50216.0
38	54540.0
39	57513.0
40	61031.5
41	64187.0
42	66292.0
43	69662.5
44	72810.5
45	76121.5
46	78920.5
47	80466.0
48	81806.5
49	82164.5
50	84762.0
51	84942.5
52	80071.0
53	76028.0
54	71749.0
55	66039.5
56	59839.0
57	53452.0
58	48102.0
59	42786.5
60	35863.0
61	29175.0
62	23282.5
63	17027.0
64	12160.5
65	9082.5
66	6650.0
67	4924.0
68	3655.0
69	2668.5
70	2054.5
71	1612.5
72	1208.0
73	1023.5
74	806.0
75	575.5
76	401.0
77	270.5
78	198.0
79	140.5
80	120.0
81	91.5
82	58.5
83	42.5
84	29.0
85	20.5
86	15.0
87	12.5
88	12.0
89	12.0
90	9.5
91	6.5
92	5.0
93	4.0
94	3.0
95	5.0
96	5.5
97	5.0
98	6.0
99	5.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03507145006387838
2	0.018645581046618884
3	0.023775582181138368
4	0.021703850953736267
5	0.01440346472384316
6	0.004784712596619132
7	0.00463673179466184
8	0.006461828352135117
9	0.0028609621711743263
10-11	0.004982020332562189
12-13	0.009150146254359268
14-15	0.0027376448362099156
16-17	0.0024910101662810945
18-19	2.4663466992882126E-5
20-21	0.005327308870462539
22-23	9.125482787366386E-4
24-25	0.0014304810855871632
26-27	0.001553798420551574
28-29	0.0035268757799821436
30-31	0.0012578368166369883
32-33	4.932693398576425E-5
34-35	0.008237597975622629
36-37	0.010358656137010492
38-39	0.0017017792225088664
40-41	0.0016524522885231023
42-43	0.0034775488459963793
44-45	0.001578461887544456
46-47	0.02451548619092483
48-49	0.004069472053825551
50-51	0.012455050831405471
52-53	0.002022404293416334
54-55	1.4798080195729273E-4
56-57	3.94615471886114E-4
58-59	0.008730867315480272
60-61	7.645674767793458E-4
62-63	0.01289899323727735
64-65	0.012652358567348528
66-67	4.932693398576425E-5
68-69	0.004957356865569307
70-71	0.006437164885142233
72-73	0.01381154151601399
74-75	0.0020717312274020983
76-77	0.001405817618594281
78-79	6.659136088078174E-4
80-81	0.005475289672419831
82-83	0.0019977408264234523
84-85	0.0014798080195729273
86-87	0.016203897814323555
88-89	0.0
90-91	7.399040097864636E-4
92-93	0.011073896679804074
94-95	0.008730867315480272
96-97	0.00604254941325612
98-99	0.0015044714865658096
100-101	0.01854692717864736
102-103	0.007990963305693809
104-105	0.008212934508629748
106-107	0.019804763995284344
108-109	0.010531300405960667
110-111	0.02357827444519531
112-113	0.002811635237188562
114-115	0.017338417295996132
116-117	0.006881107291014113
118-119	0.0059932224792703555
120-121	0.009569425193238263
122-123	0.009816059863167085
124-125	0.05610938740880683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2027290.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.300337209796766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.50671764504659	37.39825445601661
2	13.617185289319528	14.243667649593903
3	5.805698431429047	9.109199571063819
4	2.996548284431455	6.268819429648123
5	1.734600599051322	4.5360098127349815
6	1.0940824194835763	3.4332527684580816
7	0.7257815914669996	2.657103538207092
8	0.5048299396559044	2.112222086208412
9	0.3802229340035824	1.7897208896957093
>10	1.554011508603338	13.389382341542758
>50	0.05818179096913369	2.0767473213207155
>100	0.020136381800019126	1.979854656713436
>500	0.0015262359920765111	0.5416965063874427
>1k	4.7694874752390247E-4	0.4640689724089091
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2738	0.1350571452530225	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2515	0.12405723897419708	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	4.9326933985764246E-5
12-13	0.0	0.0	0.0	0.0	2.219712029359391E-4
14-15	0.0	0.0	0.0	4.9326933985764246E-5	2.7129813692170336E-4
16-17	0.0	0.0	0.0	9.865386797152849E-5	2.9596160391458546E-4
18-19	0.0	0.0	0.0	9.865386797152849E-5	2.9596160391458546E-4
20-21	0.0	0.0	0.0	1.9730773594305696E-4	3.6995200489323187E-4
22-23	0.0	0.0	0.0	6.659136088078173E-4	4.439424058718782E-4
24-25	0.0	0.0	0.0	0.0013811541516013989	4.439424058718782E-4
26-27	0.0	0.0	0.0	0.002392356298309566	4.439424058718782E-4
28-29	0.0	0.0	0.0	0.00545062620542695	4.439424058718782E-4
30-31	0.0	0.0	0.0	0.01474875326174351	4.439424058718782E-4
32-33	0.0	0.0	0.0	0.032901064968504756	4.439424058718782E-4
34-35	0.0	2.4663466992882123E-5	0.0	0.057120589555514995	4.439424058718782E-4
36-37	0.0	4.9326933985764246E-5	0.0	0.09729737728691998	4.439424058718782E-4
38-39	0.0	4.9326933985764246E-5	0.0	0.16435734404056646	4.932693398576424E-4
40-41	0.0	4.9326933985764246E-5	0.0	0.2429844768138747	4.932693398576424E-4
42-43	0.0	4.9326933985764246E-5	0.0	0.312387472931845	4.932693398576424E-4
44-45	0.0	4.9326933985764246E-5	0.0	0.39274104839465496	5.179328068505246E-4
46-47	0.0	4.9326933985764246E-5	0.0	0.49243078197988444	5.425962738434067E-4
48-49	0.0	4.9326933985764246E-5	0.0	0.594216910259509	5.919232078291709E-4
50-51	0.0	4.9326933985764246E-5	0.0	0.7078168392287241	6.905770758006994E-4
52-53	0.0	4.9326933985764246E-5	0.0	0.8195176812394872	6.905770758006994E-4
54-55	0.0	4.9326933985764246E-5	0.0	0.9364471782527414	7.892309437722279E-4
56-57	0.0	4.9326933985764246E-5	0.0	1.071701631241707	8.138944107651101E-4
58-59	0.0	4.9326933985764246E-5	0.0	1.2196824331989995	8.385578777579922E-4
60-61	0.0	4.9326933985764246E-5	0.0	1.3842124215085163	8.878848117437564E-4
62-63	0.0	4.9326933985764246E-5	0.0	1.5473859191334243	8.878848117437564E-4
64-65	0.0	4.9326933985764246E-5	0.0	1.715492110156909	9.372117457295206E-4
66-67	0.0	4.9326933985764246E-5	0.0	1.907793162300411	9.618752127224027E-4
68-69	0.0	4.9326933985764246E-5	0.0	2.119874314972204	9.865386797152848E-4
70-71	0.0	4.9326933985764246E-5	0.0	2.3652758115513812	0.001011202146708167
72-73	0.0	4.9326933985764246E-5	0.0	2.607249086218548	0.0010851925476868135
74-75	0.0	4.9326933985764246E-5	0.0	2.848235822205999	0.0011345194816725778
76-77	0.0	4.9326933985764246E-5	0.0	3.1127515057046597	0.0011345194816725778
78-79	0.0	4.9326933985764246E-5	0.0	3.4170740249298324	0.0011345194816725778
80-81	0.0	4.9326933985764246E-5	0.0	3.744432222326357	0.0011345194816725778
82-83	0.0	4.9326933985764246E-5	0.0	4.093099655204732	0.0012331733496441062
84-85	0.0	4.9326933985764246E-5	0.0	4.442630309427857	0.0012331733496441062
86-87	0.0	4.9326933985764246E-5	0.0	4.804295389411481	0.0013564906846085167
88-89	0.0	4.9326933985764246E-5	0.0	5.219899471708537	0.0015044714865658096
90-91	0.0	4.9326933985764246E-5	0.0	5.665223031732017	0.0015291349535586916
92-93	0.0	4.9326933985764246E-5	0.0	6.1211025556284495	0.0016524522885231023
94-95	0.0	4.9326933985764246E-5	0.0	6.577549339265719	0.0016771157555159845
96-97	0.0	4.9326933985764246E-5	0.0	7.053850213832259	0.0019237504254448054
98-99	0.0	4.9326933985764246E-5	0.0	7.565962442472463	0.0019730773594305697
100-101	0.0	4.9326933985764246E-5	0.0	8.137809588169429	0.0019730773594305697
102-103	0.0	4.9326933985764246E-5	0.0	8.73118794055118	0.002022404293416334
104-105	0.0	4.9326933985764246E-5	0.0	9.3149722042727	0.002022404293416334
106-107	0.0	4.9326933985764246E-5	0.0	9.901839401368328	0.002022404293416334
108-109	0.0	4.9326933985764246E-5	0.0	10.509251266469029	0.0020717312274020983
110-111	0.0	4.9326933985764246E-5	0.0	11.169196316264571	0.002170385095373627
112-113	0.0	4.9326933985764246E-5	0.0	11.868775557517672	0.002170385095373627
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	5110	0.0	29.575312	4
GTACATG	10555	0.0	27.458256	1
TACATGG	10640	0.0	26.284164	2
ACATGGG	10425	0.0	25.912363	3
GTATCAA	7350	0.0	25.019197	1
TAGACGC	250	2.0116204E-8	23.798801	5
TCAACGC	7910	0.0	22.340685	4
AACGCAG	8100	0.0	22.182833	6
CAACGCA	8125	0.0	21.968122	5
TATCAAC	8445	0.0	21.490086	2
ATCAACG	8300	0.0	21.363157	3
TATGCGT	235	7.3990086E-6	20.255297	4
ACGCAGA	9010	0.0	19.74429	7
CGCAGAG	8980	0.0	19.478975	8
ATGGGGG	2480	0.0	19.43249	5
GGTATCA	5660	0.0	18.925978	1
ATGGGGA	3080	0.0	18.54452	5
GTCTAGG	745	0.0	18.372734	1
GTATATA	1155	0.0	17.51859	1
TGTACGC	170	0.009690153	17.500847	2
>>END_MODULE