FastQCFastQC Report
Mon 15 Aug 2016
SRR2920985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027290
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.15537984205515737No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26800.1321961830818482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.12242945015266686No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23130.1140931983090727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.10186011868060316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83650.032.4787941
GGTATCA61450.027.1479571
TCAACGC104100.025.3475484
TATCAAC105900.025.3079282
ATCAACG105650.025.1441883
CAACGCA106000.024.866795
AACGCAG107650.024.701866
CATGGGG50750.023.8255544
GTACCGT2001.8299543E-623.7955726
GTACATG103550.023.3602221
TACATGG103200.022.6805152
ACATGGG101700.022.4896413
ACGCAGA120950.021.7893737
CGCAGAG121300.021.6289488
AGTACTT64450.021.46720912-13
GAGTACT59400.020.93800712-13
GTACTTT61350.020.0314-15
GTATAGA7400.019.3232651
AGAGTAC118500.019.07991610-11
GCAGAGT130350.018.986759
Produced by FastQC (version 0.11.5)