FastQCFastQC Report
Mon 15 Aug 2016
SRR2920984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123756
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64650.033.780291
GGTATCA46700.031.8558311
TCAACGC75550.028.1950364
CAACGCA76700.027.6938325
GTACATG116500.027.3271081
AACGCAG78100.027.2731466
ATCAACG80700.026.6906493
TACATGG116250.026.4619392
TATCAAC83100.026.277812
ACATGGG119300.025.6357943
CATGGGG79150.025.0332584
ACGCAGA90300.023.4562477
CGCAGAG93000.022.5198
GCAGAGT103300.019.4672999
GTATAGC11700.019.326961
AGAGTAC94000.018.0388910-11
ATATAGG10100.017.0860791
ATGGGGG43650.017.0386815
ATACGGC3506.9887756E-716.9994646
GTTCGCG3506.98994E-716.9991917
Produced by FastQC (version 0.11.5)