##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920984_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3123756
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.81135850559391	31.0	30.0	33.0	22.0	34.0
2	28.927372048264974	31.0	30.0	34.0	22.0	34.0
3	29.03884138197734	31.0	30.0	34.0	22.0	34.0
4	32.461480346096174	35.0	33.0	37.0	24.0	37.0
5	32.593406143117456	35.0	33.0	37.0	25.0	37.0
6	32.56953199929828	35.0	33.0	37.0	25.0	37.0
7	32.40051047520997	35.0	33.0	37.0	23.0	37.0
8	32.471139551232554	35.0	33.0	37.0	23.0	37.0
9	33.65510366366643	38.0	33.0	39.0	22.0	39.0
10-11	33.60972639988527	37.5	33.0	39.0	21.5	39.0
12-13	33.57166836974463	38.0	33.0	39.0	22.0	39.0
14-15	34.52716425354605	39.0	33.0	41.0	20.0	41.0
16-17	34.40141499528132	39.0	33.0	41.0	18.5	41.0
18-19	34.52131168375507	39.0	33.5	41.0	20.0	41.0
20-21	34.541752620883315	39.0	34.0	41.0	20.0	41.0
22-23	34.5151754810555	39.0	34.0	41.0	21.5	41.0
24-25	34.448733351772674	39.0	33.5	41.0	20.5	41.0
26-27	34.30555571561928	39.0	33.0	41.0	19.5	41.0
28-29	34.23860794505077	39.0	33.0	41.0	19.0	41.0
30-31	34.116764881764134	38.0	33.0	41.0	18.5	41.0
32-33	34.014443669736046	38.0	33.0	41.0	18.5	41.0
34-35	33.84336468661445	38.0	33.0	40.0	17.0	41.0
36-37	33.71442375780951	38.0	33.0	40.0	16.0	41.0
38-39	33.59761998056186	38.0	33.0	40.0	16.0	41.0
40-41	33.51743494050112	38.0	32.5	40.0	15.0	41.0
42-43	33.31938970265283	38.0	32.0	40.0	13.5	41.0
44-45	33.171963495228184	38.0	32.0	40.0	11.0	41.0
46-47	32.9894145061266	38.0	32.0	40.0	9.0	41.0
48-49	32.83151500949498	38.0	31.5	40.0	8.5	41.0
50-51	32.621091083938694	38.0	31.0	40.0	7.5	41.0
52-53	32.446611547124675	37.0	31.0	40.0	6.5	41.0
54-55	32.23984203631782	37.0	31.0	40.0	2.0	41.0
56-57	32.055564038932616	37.0	31.0	40.0	2.0	41.0
58-59	31.807296728681756	36.5	30.0	40.0	2.0	41.0
60-61	31.62901919996312	36.0	30.0	40.0	2.0	41.0
62-63	31.517611970973405	36.0	30.0	39.5	2.0	41.0
64-65	31.30006969174289	35.5	30.0	39.0	2.0	41.0
66-67	31.039075555197012	35.0	30.0	39.0	2.0	41.0
68-69	30.731962419599995	35.0	30.0	39.0	2.0	40.0
70-71	30.40599281762084	35.0	29.0	38.0	2.0	40.0
72-73	30.115006421756373	35.0	29.0	37.0	2.0	40.0
74-75	29.797290345340674	35.0	29.0	37.0	2.0	39.0
76-77	29.494517657589135	34.0	29.0	36.0	2.0	39.0
78-79	29.157789692920957	34.0	29.0	36.0	2.0	38.5
80-81	28.825138711218163	34.0	29.0	35.5	2.0	37.0
82-83	28.5388322583454	34.0	28.0	35.0	2.0	37.0
84-85	28.284564159300537	34.0	28.0	35.0	2.0	36.5
86-87	28.19810318091426	34.0	28.0	35.0	2.0	36.0
88-89	28.275615477009087	34.0	29.0	35.0	2.0	36.0
90-91	28.173969413744224	34.0	29.0	35.0	2.0	35.5
92-93	28.01485327279083	34.0	28.0	35.0	2.0	35.0
94-95	27.930797571897422	34.0	28.0	35.0	2.0	35.0
96-97	27.8272044935648	34.0	28.0	35.0	2.0	35.0
98-99	27.75928337552613	34.0	27.5	35.0	2.0	35.0
100-101	27.586449773926006	34.0	27.0	35.0	2.0	35.0
102-103	26.262580047865455	31.5	24.5	34.0	2.0	35.0
104-105	27.26163663231059	33.5	26.5	35.0	2.0	35.0
106-107	27.43797739003943	34.0	27.0	35.0	2.0	35.0
108-109	27.42565072303983	34.0	27.0	35.0	2.0	35.0
110-111	27.313335932768116	34.0	27.0	35.0	2.0	35.0
112-113	27.215475056310417	34.0	27.0	35.0	2.0	35.0
114-115	27.07512590612071	34.0	26.0	35.0	2.0	35.0
116-117	26.938213804151154	34.0	25.0	35.0	2.0	35.0
118-119	26.74065291911404	34.0	25.0	35.0	2.0	35.0
120-121	26.519126173747246	34.0	25.0	35.0	2.0	35.0
122-123	26.282689332969667	34.0	25.0	35.0	2.0	35.0
124-125	25.11693759051603	32.5	20.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	149033.0
3	21502.0
4	13722.0
5	12553.0
6	14544.0
7	16169.0
8	17245.0
9	18240.0
10	19511.0
11	20487.0
12	20880.0
13	21205.0
14	22041.0
15	20005.0
16	16529.0
17	14717.0
18	13935.0
19	13227.0
20	13812.0
21	14925.0
22	18112.0
23	23774.0
24	32519.0
25	41621.0
26	48256.0
27	55357.0
28	65077.0
29	75559.0
30	89468.0
31	105787.0
32	130305.0
33	164263.0
34	213646.0
35	295292.0
36	463163.0
37	624485.0
38	202594.0
39	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.998060632766595	19.868010304009264	13.723296873058551	24.41063219016559
2	15.615593596962096	20.439548476644983	39.97649850073882	23.968359425654096
3	18.64789123853913	24.701362592210483	29.85599186428	26.794754304970386
4	12.847835261224258	16.745082293181586	36.98367840691723	33.42340403867693
5	13.78494566506783	37.77773793404632	34.70052473127093	13.736791669614925
6	32.34779913939568	36.602831523306776	17.450911906989937	13.598457430307601
7	27.95874658249083	32.75091080221026	21.43032763268259	17.860014982616324
8	25.527758462383645	36.14663649623446	20.084723081277044	18.240881960104854
9	26.022787298274146	16.602490019752405	19.78877410225792	37.58594857971553
10-11	24.719107058819763	26.57679735946549	28.110631608939656	20.59346397277509
12-13	25.214757709251103	24.181404313082677	27.82386538264522	22.779972595021004
14-15	22.618757211036712	25.708394319015625	25.226735482846536	26.446112987101127
16-17	22.72348569186721	28.534295356253516	26.12373709281202	22.61848185906725
18-19	22.70328862271347	27.787987180170532	27.486699098792815	22.022025098323187
20-21	23.805573892459872	26.86558348385246	27.056610985794112	22.272231637893555
22-23	23.332375611548045	26.699293233335442	26.826688905019875	23.141642250096638
24-25	22.990965420881928	26.856708210609753	27.096886549228717	23.055439819279606
26-27	22.913691439444943	26.970404752552334	27.023754758362728	23.092149049639996
28-29	22.865930618304635	27.19018139763819	26.992928973600698	22.950959010456476
30-31	23.254462703639213	26.99917694366626	26.807482609693622	22.938877743000898
32-33	22.98835120284683	27.002733248051385	26.672969975888	23.335945573213785
34-35	23.256718927481558	27.057297369287138	26.742000484062434	22.943983219168874
36-37	23.290596888226744	26.799709750956012	26.771744546905268	23.137948813911976
38-39	23.102573045741597	26.99208201272173	26.82988708460659	23.075457856930086
40-41	23.329840169759105	26.851970270707636	26.83777245755502	22.980417101978233
42-43	23.19300925768822	26.863574752038783	26.65923204534148	23.284183944931513
44-45	23.153096986047895	26.932001802336796	26.73518534376853	23.179715867846777
46-47	23.190306978138125	26.944508660155726	26.62419144236884	23.240992919337312
48-49	23.074405072514338	27.0197633671804	26.650836935413547	23.254994624891715
50-51	23.119448662524462	27.123583181338383	26.611714768786783	23.14525338735037
52-53	23.274495570560898	26.973199272337872	26.503964446037998	23.248340711063236
54-55	23.10374240716651	26.9264216439752	26.59616169852179	23.373674250336496
56-57	23.10691415987648	27.00966050729428	26.46933220306753	23.414093129761707
58-59	23.119899332474162	26.826211654401504	26.630104629364766	23.423784383759568
60-61	23.136410513498816	26.898155462216483	26.744845475922823	23.220588548361874
62-63	23.088111350784583	26.8956486591403	26.76478427386274	23.251455716212377
64-65	23.046435407947026	26.81205472013862	26.66334129139604	23.478168580518314
66-67	23.03939426728225	26.937140376610962	26.5513545560886	23.472110800018182
68-69	23.265407717764948	26.816219871864135	26.593082584006627	23.325289826364294
70-71	23.0335252217511	26.652470455997374	26.75872593944782	23.555278382803706
72-73	23.153908868962976	26.779841285315843	26.643115434813335	23.423134410907846
74-75	23.148754920054614	26.928173205130463	26.481186543500566	23.441885331314356
76-77	23.170949357616365	26.70886531832664	26.613514186222027	23.506671137834967
78-79	23.218532287043967	26.738594392134424	26.626725196979578	23.41614812384203
80-81	23.223924231694685	26.84386553072261	26.513480478469265	23.41872975911344
82-83	23.274064496177534	26.78835337977712	26.594400974868144	23.3431811491772
84-85	23.27249241289232	26.786619799469864	26.486016672429024	23.45487111520879
86-87	23.14189627257065	26.722176349244513	26.5768840533541	23.559043324830736
88-89	23.147022366663723	26.623366229628694	26.571745680520504	23.657865723187086
90-91	23.07719149301254	26.704064169274123	26.66891397421935	23.549830363493992
92-93	23.20503525778579	26.534095065638823	26.712917214997496	23.54795246157789
94-95	23.096016207169008	26.60939967140016	26.7343854248171	23.560198696613732
96-97	23.129694781478317	26.488303854396882	26.760040331481367	23.621961032643433
98-99	23.01445071901451	26.559166634653554	26.60118384490287	23.825198801429064
100-101	22.96986878737946	26.560185983399876	26.750674249056967	23.719270980163703
102-103	22.861573294001676	26.56810915110918	26.804909072223897	23.765408482665247
104-105	22.832122149096197	26.722900106194054	26.572861383941937	23.872116360767812
106-107	22.794328237161153	26.797512124080658	26.51483970182758	23.893319936930606
108-109	22.779082261216566	26.77706075655628	26.638382463303	23.805474518924154
110-111	22.733439944569415	26.98909494809066	26.53535949113493	23.74210561620499
112-113	22.71189164547366	27.160037929718612	26.517156730924373	23.610913693883354
114-115	22.726324587408897	27.206511745997595	26.399090973270447	23.66807269332306
116-117	22.728024342616337	27.10211422774958	26.450249081541326	23.719612348092756
118-119	22.788993251720523	27.066814657779087	26.33285642802513	23.81133566247526
120-121	22.73415961093975	27.161847354264484	26.449431435910657	23.654561598885106
122-123	22.748800903090494	27.244499873779272	26.284245876376044	23.72245334675419
124-125	22.780880952960576	27.35290010032378	26.170748379508325	23.695470567207323
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	561.0
1	482.5
2	331.0
3	248.5
4	236.5
5	230.0
6	210.5
7	231.0
8	274.5
9	325.5
10	356.5
11	345.0
12	396.5
13	476.0
14	554.5
15	659.5
16	793.5
17	939.5
18	1084.5
19	1347.0
20	1701.5
21	2112.5
22	2683.0
23	3432.5
24	4477.5
25	5943.5
26	8239.0
27	11328.0
28	14928.5
29	19985.5
30	25991.5
31	33088.5
32	41266.0
33	48084.5
34	53853.0
35	60108.5
36	68106.0
37	76299.5
38	83659.0
39	90134.5
40	95721.0
41	101876.5
42	107524.5
43	113661.5
44	119252.0
45	123797.5
46	128002.0
47	129844.0
48	131176.0
49	131283.5
50	129130.5
51	125620.5
52	119729.5
53	114652.5
54	110111.0
55	102801.0
56	93460.0
57	83389.5
58	75013.0
59	66272.0
60	55719.0
61	46315.5
62	38006.0
63	28889.5
64	20928.5
65	15782.0
66	11816.0
67	8902.0
68	6798.0
69	5242.5
70	4176.5
71	3195.0
72	2436.0
73	2083.5
74	1674.5
75	1222.5
76	919.5
77	631.0
78	422.5
79	274.0
80	181.0
81	140.0
82	93.0
83	60.0
84	45.5
85	37.0
86	26.5
87	20.0
88	19.5
89	15.5
90	15.0
91	17.0
92	14.0
93	9.5
94	8.5
95	10.5
96	8.5
97	9.0
98	7.5
99	10.0
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034829865072688135
2	0.017639021741774966
3	0.02362540480114324
4	0.020936334335972463
5	0.014181645429412542
6	0.0043857458777190024
7	0.004353733134086017
8	0.00537814093034155
9	0.0027530959524367463
10-11	0.004673860570415871
12-13	0.00755500749738456
14-15	0.00230491754157495
16-17	0.0019367709897956176
18-19	4.8019115449478125E-5
20-21	0.0039055547232242213
22-23	7.522994753751574E-4
24-25	0.0011364523989709824
26-27	0.001440573463484344
28-29	0.0029931915296841365
30-31	0.0010564205398885188
32-33	0.0
34-35	0.0061464467775332
36-37	0.007410950151036124
38-39	0.0014565798353008365
40-41	0.0011364523989709824
42-43	0.0026250449779048044
44-45	0.0010724269117050116
46-47	0.019271671667057223
48-49	0.003137248876032571
50-51	0.00950778485899667
52-53	0.00171268178436472
54-55	1.4405734634843437E-4
56-57	2.2408920543089792E-4
58-59	0.006610631560211489
60-61	6.242485008432156E-4
62-63	0.009395740256281222
64-65	0.00957181034626264
66-67	6.402548726597083E-5
68-69	0.003793510120508772
70-71	0.004881943404030277
72-73	0.01128449213062736
74-75	0.0019527773616121106
76-77	0.0012324906298699387
78-79	5.922357572102303E-4
80-81	0.004097631185022133
82-83	0.001648656297098749
84-85	0.001152458770787475
86-87	0.01270905922229521
88-89	0.0
90-91	5.762293853937375E-4
92-93	0.008371332460025688
94-95	0.006402548726597084
96-97	0.004529803224067437
98-99	0.001152458770787475
100-101	0.014933944904787697
102-103	0.007458969266485603
104-105	0.007330918291953661
106-107	0.016950747753665778
108-109	0.010884332835215043
110-111	0.01826327024261818
112-113	0.002689070465170775
114-115	0.01330129497950544
116-117	0.005778300225753868
118-119	0.004641847826782886
120-121	0.007346924663770154
122-123	0.008307306972759715
124-125	0.05967175413188482
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3123756.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.66797251119633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50597139914808	41.654343666557075
2	13.988073961312859	15.853515814485164
3	5.329621534015844	9.060565397540698
4	2.4978206831687935	5.661857352468274
5	1.395524229909959	3.9540764349622988
6	0.814452931681276	2.7692037806506686
7	0.559181508389735	2.2181377642339166
8	0.36168708737887806	1.6396859140192743
9	0.2711975582666275	1.3831394199261133
>10	1.2061496213043386	11.669664197208737
>50	0.05252359885403084	2.0154229479403463
>100	0.01660054490796543	1.5935501522015232
>500	9.676575357883364E-4	0.3788143992359054
>1k	2.276841258232782E-4	0.1480227585699337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.201274363298542E-5	0.0	0.0	0.0
3	0.0	3.201274363298542E-5	0.0	0.0	0.0
4	0.0	3.201274363298542E-5	0.0	0.0	0.0
5	0.0	3.201274363298542E-5	0.0	0.0	0.0
6	0.0	3.201274363298542E-5	0.0	0.0	0.0
7	0.0	3.201274363298542E-5	0.0	0.0	0.0
8	0.0	3.201274363298542E-5	0.0	0.0	0.0
9	0.0	3.201274363298542E-5	0.0	0.0	0.0
10-11	0.0	3.201274363298542E-5	0.0	0.0	0.0
12-13	0.0	3.201274363298542E-5	0.0	3.201274363298542E-5	4.801911544947813E-5
14-15	0.0	3.201274363298542E-5	0.0	3.201274363298542E-5	8.003185908246355E-5
16-17	0.0	3.201274363298542E-5	0.0	3.201274363298542E-5	9.603823089895625E-5
18-19	0.0	3.201274363298542E-5	0.0	9.603823089895625E-5	9.603823089895625E-5
20-21	0.0	3.201274363298542E-5	0.0	1.2805097453194168E-4	1.440573463484344E-4
22-23	0.0	3.201274363298542E-5	0.0	2.2408920543089795E-4	1.600637181649271E-4
24-25	0.0	3.201274363298542E-5	0.0	5.762293853937375E-4	1.600637181649271E-4
26-27	0.0	3.201274363298542E-5	0.0	0.0014085607198513583	1.920764617979125E-4
28-29	0.0	3.201274363298542E-5	0.0	0.0037614973768757866	1.920764617979125E-4
30-31	0.0	3.201274363298542E-5	0.0	0.014213658173045526	1.920764617979125E-4
32-33	0.0	3.201274363298542E-5	0.0	0.038447305103215486	1.920764617979125E-4
34-35	0.0	3.201274363298542E-5	0.0	0.06917953899088149	1.920764617979125E-4
36-37	0.0	3.201274363298542E-5	0.0	0.12049596703455712	1.920764617979125E-4
38-39	0.0	3.201274363298542E-5	0.0	0.20147220205419375	1.920764617979125E-4
40-41	0.0	3.201274363298542E-5	0.0	0.2911719097138189	1.920764617979125E-4
42-43	0.0	3.201274363298542E-5	0.0	0.3796711394872071	2.080828336144052E-4
44-45	0.0	3.201274363298542E-5	0.0	0.4841287219616385	2.2408920543089792E-4
46-47	0.0	3.201274363298542E-5	0.0	0.605649096792451	2.2408920543089792E-4
48-49	0.0	3.201274363298542E-5	0.0	0.7293463381903068	2.2408920543089792E-4
50-51	0.0	3.201274363298542E-5	0.0	0.8505145728411566	2.2408920543089792E-4
52-53	0.0	3.201274363298542E-5	0.0	0.9774931204613933	2.2408920543089792E-4
54-55	0.0	3.201274363298542E-5	0.0	1.121790562387075	2.881146926968688E-4
56-57	0.0	3.201274363298542E-5	0.0	1.2889931223821578	2.881146926968688E-4
58-59	0.0	3.201274363298542E-5	0.0	1.4669679706097405	2.881146926968688E-4
60-61	0.0	3.201274363298542E-5	0.0	1.6460952776081101	2.881146926968688E-4
62-63	0.0	6.402548726597084E-5	0.0	1.8310489039476834	3.201274363298542E-4
64-65	0.0	6.402548726597084E-5	0.0	2.0369228582514127	3.201274363298542E-4
66-67	0.0	6.402548726597084E-5	0.0	2.2743773841490817	3.361338081463469E-4
68-69	0.0	6.402548726597084E-5	0.0	2.535985525117839	3.5214017996283963E-4
70-71	0.0	6.402548726597084E-5	0.0	2.8116632669132926	3.681465517793323E-4
72-73	0.0	6.402548726597084E-5	0.0	3.0883334037613692	3.84152923595825E-4
74-75	0.0	6.402548726597084E-5	0.0	3.386900257254408	3.84152923595825E-4
76-77	0.0	6.402548726597084E-5	0.0	3.724282562402441	3.84152923595825E-4
78-79	0.0	6.402548726597084E-5	0.0	4.090492343191978	3.84152923595825E-4
80-81	0.0	6.402548726597084E-5	0.0	4.475077438826848	4.161656672288104E-4
82-83	0.0	6.402548726597084E-5	0.0	4.873844179891131	4.4817841086179584E-4
84-85	0.0	6.402548726597084E-5	0.0	5.296188306641108	4.4817841086179584E-4
86-87	0.0	6.402548726597084E-5	0.0	5.758260248239619	4.641847826782886E-4
88-89	0.0	6.402548726597084E-5	0.0	6.253081226574675	4.96197526311274E-4
90-91	0.0	6.402548726597084E-5	0.0	6.768518411809373	5.122038981277667E-4
92-93	0.0	6.402548726597084E-5	0.0	7.295640248470111	5.442166417607521E-4
94-95	0.0	6.402548726597084E-5	0.0	7.843698419466821	5.442166417607521E-4
96-97	0.0	6.402548726597084E-5	0.0	8.431004214157571	5.442166417607521E-4
98-99	0.0	6.402548726597084E-5	0.0	9.059862550083938	5.762293853937376E-4
100-101	0.0	6.402548726597084E-5	0.0	9.738628753334126	5.762293853937376E-4
102-103	0.0	6.402548726597084E-5	0.0	10.41542617285089	5.762293853937376E-4
104-105	0.0	6.402548726597084E-5	0.0	11.098017898965221	5.762293853937376E-4
106-107	0.0	8.003185908246355E-5	0.0	11.821697981532488	6.08242129026723E-4
108-109	0.0	9.603823089895625E-5	0.0	12.581088279622353	6.402548726597084E-4
110-111	0.0	9.603823089895625E-5	0.0	13.383423673295866	6.722676162926938E-4
112-113	0.0	9.603823089895625E-5	0.0	14.185198843955803	6.722676162926938E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6465	0.0	33.78029	1
GGTATCA	4670	0.0	31.855831	1
TCAACGC	7555	0.0	28.195036	4
CAACGCA	7670	0.0	27.693832	5
GTACATG	11650	0.0	27.327108	1
AACGCAG	7810	0.0	27.273146	6
ATCAACG	8070	0.0	26.690649	3
TACATGG	11625	0.0	26.461939	2
TATCAAC	8310	0.0	26.27781	2
ACATGGG	11930	0.0	25.635794	3
CATGGGG	7915	0.0	25.033258	4
ACGCAGA	9030	0.0	23.456247	7
CGCAGAG	9300	0.0	22.519	8
GCAGAGT	10330	0.0	19.467299	9
GTATAGC	1170	0.0	19.32696	1
AGAGTAC	9400	0.0	18.03889	10-11
ATATAGG	1010	0.0	17.086079	1
ATGGGGG	4365	0.0	17.038681	5
ATACGGC	350	6.9887756E-7	16.999464	6
GTTCGCG	350	6.98994E-7	16.999191	7
>>END_MODULE