FastQCFastQC Report
Mon 15 Aug 2016
SRR2920984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123756
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.11530990256601346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.10221669042012245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.046.2772331
GTATCAA87950.042.7610131
TCAACGC111650.033.4560974
CAACGCA113950.032.789455
ATCAACG114400.032.5992623
TATCAAC115850.032.1871152
AACGCAG117150.031.7886586
ACGCAGA132400.027.7704147
CGCAGAG136700.026.7232138
GTACATG116900.026.715991
TACATGG117750.025.7996062
CATGGGG78200.024.8357494
ACATGGG119150.024.499743
GCAGAGT152250.023.5639929
AGAGTAC139450.022.84685110-11
GAGTACT82300.021.68962312-13
AGTACTT89300.020.95558412-13
GTACTTT89050.020.07895714-15
CAGAGTA148350.019.95220610-11
ATGGGGG38900.018.8124085
Produced by FastQC (version 0.11.5)