FastQCFastQC Report
Mon 15 Aug 2016
SRR2920983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584311
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71850.033.799841
GTACATG125150.030.1536351
CATGGGG78000.029.7537674
TCAACGC82100.029.5725654
CAACGCA84050.028.8842325
TACATGG127300.028.704212
ATCAACG85350.028.657873
AACGCAG85950.028.2429876
ACATGGG128350.027.7275123
TATCAAC87850.027.6391072
GGTATCA50150.027.4171491
ACGCAGA97300.024.5815667
CGCAGAG99100.023.954978
GCAGAGT107350.021.282649
ATGGGGA42950.020.919545
GTATAAT14200.020.5394571
ATGGGGG45100.020.450015
AGAGTAC93250.018.98222410-11
CAGAGTA100450.017.74009110-11
GTGTAGC15050.017.4019321
Produced by FastQC (version 0.11.5)