##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920983_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2584311 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.483373324650167 31.0 28.0 33.0 16.0 34.0 2 28.59243798443763 31.0 28.0 34.0 16.0 34.0 3 28.713077102562348 31.0 28.0 34.0 16.0 34.0 4 32.10702310983469 35.0 32.0 37.0 20.0 37.0 5 32.244280196926766 35.0 33.0 37.0 22.0 37.0 6 32.210827953756336 35.0 33.0 37.0 21.0 37.0 7 32.0402730166764 35.0 32.0 37.0 19.0 37.0 8 32.108215690758584 35.0 33.0 37.0 19.0 37.0 9 33.24261940610089 37.0 33.0 39.0 18.0 39.0 10-11 33.18914441798994 37.0 33.0 39.0 17.5 39.0 12-13 33.14007215075894 37.0 33.0 39.0 17.0 39.0 14-15 34.05428061870263 38.0 33.0 41.0 17.0 41.0 16-17 33.92885357064223 38.0 33.0 41.0 17.0 41.0 18-19 34.04680473828421 38.5 33.0 41.0 17.5 41.0 20-21 34.06158063019505 39.0 33.0 41.0 18.0 41.0 22-23 34.03835122785145 39.0 33.0 41.0 18.0 41.0 24-25 33.972909413766374 38.5 33.0 41.0 18.0 41.0 26-27 33.82745497736147 38.0 32.5 41.0 17.0 41.0 28-29 33.763764113529675 38.0 32.5 41.0 16.5 41.0 30-31 33.63688387349665 38.0 32.0 40.0 16.0 41.0 32-33 33.53264506477742 38.0 32.0 40.0 15.5 41.0 34-35 33.35885560987049 38.0 32.0 40.0 14.5 41.0 36-37 33.22700460586981 38.0 32.0 40.0 9.0 41.0 38-39 33.09511393946007 38.0 32.0 40.0 9.0 41.0 40-41 33.00905076827054 38.0 32.0 40.0 9.0 41.0 42-43 32.802748005174294 38.0 31.0 40.0 8.0 41.0 44-45 32.645986686586866 38.0 31.0 40.0 7.5 41.0 46-47 32.44660878663598 38.0 31.0 40.0 4.0 41.0 48-49 32.27632084528526 37.5 31.0 40.0 2.0 41.0 50-51 32.05200709202569 37.0 30.0 40.0 2.0 41.0 52-53 31.87886597240038 37.0 30.0 40.0 2.0 41.0 54-55 31.66193832708215 37.0 30.0 40.0 2.0 41.0 56-57 31.463041406394197 36.0 30.0 40.0 2.0 41.0 58-59 31.207090207022297 36.0 29.5 40.0 2.0 41.0 60-61 31.01382883097274 36.0 29.5 39.0 2.0 41.0 62-63 30.89048763867816 35.5 29.0 39.0 2.0 41.0 64-65 30.670278461067575 35.0 29.0 39.0 2.0 41.0 66-67 30.411314466409035 35.0 29.0 39.0 2.0 40.5 68-69 30.10352778748378 35.0 29.0 38.0 2.0 40.0 70-71 29.7696335696439 35.0 29.0 37.5 2.0 40.0 72-73 29.49481505902347 34.5 29.0 37.0 2.0 39.5 74-75 29.18728628249464 34.0 28.0 36.5 2.0 39.0 76-77 28.89514632720288 34.0 28.0 36.0 2.0 39.0 78-79 28.57099571220337 34.0 28.0 35.5 2.0 37.5 80-81 28.251666885293602 34.0 27.0 35.0 2.0 37.0 82-83 27.987395673353554 34.0 27.0 35.0 2.0 37.0 84-85 27.745452462958212 34.0 26.0 35.0 2.0 36.0 86-87 27.66865501094876 34.0 26.5 35.0 2.0 36.0 88-89 27.751460060341035 34.0 27.0 35.0 2.0 36.0 90-91 27.65810751879321 34.0 27.0 35.0 2.0 35.0 92-93 27.508408624194225 34.0 27.0 35.0 2.0 35.0 94-95 27.43379105688131 34.0 27.0 35.0 2.0 35.0 96-97 27.33895049783095 34.0 27.0 35.0 2.0 35.0 98-99 27.281100068838462 34.0 27.0 35.0 2.0 35.0 100-101 27.110936145069225 34.0 26.0 35.0 2.0 35.0 102-103 25.791405910511543 31.5 24.0 34.0 2.0 35.0 104-105 26.794151710068952 33.5 25.0 35.0 2.0 35.0 106-107 26.981661843330777 34.0 25.0 35.0 2.0 35.0 108-109 26.97585275146838 34.0 25.0 35.0 2.0 35.0 110-111 26.880093765804503 34.0 25.0 35.0 2.0 35.0 112-113 26.79364422470825 34.0 25.0 35.0 2.0 35.0 114-115 26.663896876188662 34.0 25.0 35.0 2.0 35.0 116-117 26.540199496113274 34.0 25.0 35.0 2.0 35.0 118-119 26.366170712425866 34.0 25.0 35.0 2.0 35.0 120-121 26.159439014886367 34.0 24.0 35.0 2.0 35.0 122-123 25.945149209982855 34.0 23.5 35.0 2.0 35.0 124-125 24.81301766699132 32.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 140632.0 3 20161.0 4 12872.0 5 11535.0 6 13035.0 7 14466.0 8 15343.0 9 16208.0 10 17115.0 11 18023.0 12 18231.0 13 18714.0 14 19555.0 15 17676.0 16 14432.0 17 13044.0 18 11994.0 19 11542.0 20 11785.0 21 12624.0 22 15345.0 23 20433.0 24 27868.0 25 36420.0 26 41829.0 27 46927.0 28 54897.0 29 64145.0 30 74440.0 31 89516.0 32 109538.0 33 136773.0 34 178483.0 35 244509.0 36 376078.0 37 484657.0 38 153254.0 39 212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25928552405844 19.060020686109958 13.282929004527407 24.39776478530419 2 15.596907253201808 20.26714378377709 39.726772276430694 24.40917668659041 3 19.042725713998195 24.9582573760784 29.305605548653286 26.69341136127012 4 12.981924749802031 16.458919850728822 36.369793718751865 34.18936168071728 5 13.863947636980589 38.03087585300243 34.01719298394983 14.087983526067148 6 32.63024981154622 36.16617676422923 17.37536123532808 13.828212188896474 7 28.374173480882337 32.7338913179843 21.08470730184921 17.807227899284143 8 26.34463319250109 35.7794906046912 19.69512747265845 18.180748730149272 9 26.549881396005777 16.808411016047334 19.196859412668378 37.444848175278516 10-11 25.18488893893688 26.633468598338396 27.396242492560035 20.785399970164693 12-13 25.70839470264489 23.979250658454337 27.516328816190814 22.79602582270996 14-15 22.963521189982355 25.3199006284246 24.8215722997864 26.895005881806643 16-17 23.09331830893481 28.422445894171634 25.575130183291726 22.909105613601827 18-19 23.355397890462466 27.4262709531227 27.0604673857882 22.157863770626633 20-21 24.180203928148597 26.58066178842188 26.708980303146685 22.53015398028283 22-23 23.75236888310608 26.28490444658344 26.665841165118042 23.296885505192435 24-25 23.395612615230664 26.500180128028266 26.74316916208156 23.361038094659502 26-27 23.299027634361728 26.674572204199688 26.628814504088105 23.397585657350476 28-29 23.36485486390922 26.92603646178478 26.561650793036563 23.14745788126944 30-31 23.607318410526425 26.716060236576805 26.463629866303034 23.212991486593737 32-33 23.31716267895002 26.718301318997597 26.468079886669987 23.496456115382397 34-35 23.504424408906594 26.760239082352843 26.559784809629388 23.17555169911117 36-37 23.701637061686533 26.52380091800528 26.481077799031844 23.293484221276344 38-39 23.47161058784811 26.720175121393343 26.451278497228124 23.356935793530422 40-41 23.572811251157244 26.651633393214908 26.519797195917217 23.255758159710634 42-43 23.524283369014533 26.646705833754964 26.387634215640844 23.44137658158966 44-45 23.525612760438786 26.65053960143847 26.43908711498051 23.384760523142237 46-47 23.589612494668618 26.696114729011 26.32506604720721 23.389206729113177 48-49 23.344021920730064 26.94290212464455 26.23522257485381 23.47785337977158 50-51 23.306688802718245 26.999229882121657 26.324409253798347 23.369672061361754 52-53 23.60506003480312 26.756650186069386 26.18870999759698 23.44957978153051 54-55 23.44913996319324 26.720479911837135 26.256912875709858 23.573467249259764 56-57 23.436226989680968 26.80491157672138 26.139703072685954 23.619158360911698 58-59 23.446912361023692 26.641328561364347 26.263075783869816 23.64868329374214 60-61 23.361108821639032 26.680952972437723 26.482271940526868 23.475666265396377 62-63 23.357184306770254 26.65878879572482 26.561324813571353 23.422702083933576 64-65 23.263078622273923 26.615173376738472 26.48011281620231 23.641635184785294 66-67 23.40662889124886 26.787243086496833 26.18582495085242 23.620303071401885 68-69 23.608427583052425 26.515770373669646 26.3704632144575 23.505338828820427 70-71 23.364465186113918 26.453484097408452 26.457527949012643 23.72452276746499 72-73 23.478845743403546 26.539604451808614 26.302741484223873 23.678808320563967 74-75 23.34061522751066 26.6609809719481 26.338858247233688 23.65954555330755 76-77 23.4982994286413 26.368675047987285 26.476654896134455 23.656370627236964 78-79 23.570828241678385 26.51778396730403 26.36108739949866 23.55030039151892 80-81 23.503762590257953 26.556420666355674 26.30996050794243 23.629856235443945 82-83 23.529054397087595 26.499541841055997 26.370278331558485 23.601125430297927 84-85 23.484589781546124 26.5629186981032 26.246485717176824 23.70600580317385 86-87 23.405539027163 26.51136105265704 26.400793362842563 23.682306557337395 88-89 23.347673712645268 26.418859804412087 26.369852544836903 23.863613938105747 90-91 23.337378283878138 26.487351274546374 26.416616233769247 23.758654207806238 92-93 23.424088976778656 26.376881981544436 26.501125173903706 23.697903867773203 94-95 23.28550705765371 26.389187455098167 26.57031368059287 23.75499180665525 96-97 23.44105499026672 26.19736933501614 26.59393682820691 23.76763884651023 98-99 23.27251740189264 26.322213960712748 26.456952021525588 23.948316615869025 100-101 23.122001248119357 26.402213718670026 26.55930260121619 23.916482431994428 102-103 23.091671661995118 26.322452740452025 26.634865547945285 23.951010049607575 104-105 23.077321966373173 26.427079473587973 26.36400135830788 24.131597201730973 106-107 22.99925537127328 26.447323709922994 26.439389775984406 24.114031142819325 108-109 23.042042930503264 26.304622634517973 26.588734927274952 24.06459950770381 110-111 22.93819839429302 26.638198084669618 26.41733983955315 24.00626368148421 112-113 23.00937760472897 26.72083342039948 26.41327708473123 23.856511890140318 114-115 22.992181658593676 26.79771154259955 26.32080123099596 23.88930556781082 116-117 22.978659924029238 26.64729087143481 26.403168234685886 23.970880969850068 118-119 23.079722658370024 26.53457434715688 26.23956752196782 24.146135472505275 120-121 22.973342437768675 26.65549186879629 26.431795969836173 23.939369723598862 122-123 23.03308466062512 26.65299950368655 26.279342234726265 24.034573600962055 124-125 23.06454975732643 26.72957795067002 26.15860636005901 24.04726593194454 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 854.0 1 614.0 2 335.5 3 281.0 4 227.5 5 198.5 6 215.5 7 236.5 8 298.5 9 368.0 10 400.0 11 410.5 12 422.0 13 487.5 14 593.5 15 705.0 16 810.0 17 918.5 18 1072.0 19 1229.0 20 1468.0 21 1822.5 22 2416.5 23 3301.0 24 4297.0 25 5582.5 26 7527.0 27 10417.5 28 13469.5 29 16763.0 30 20794.0 31 25913.5 32 31596.0 33 36323.5 34 40556.0 35 45687.0 36 51683.5 37 57322.0 38 63289.0 39 68218.5 40 73150.5 41 78578.5 42 83428.0 43 88586.5 44 93046.5 45 97131.5 46 101100.0 47 104247.5 48 105887.5 49 105700.5 50 105705.5 51 105051.5 52 101321.5 53 97940.5 54 95810.5 55 90882.5 56 84056.0 57 76665.0 58 69539.0 59 62213.0 60 52464.0 61 43666.0 62 35815.0 63 27061.0 64 19842.5 65 15438.5 66 12214.5 67 9332.5 68 7109.5 69 5390.5 70 4211.0 71 3284.5 72 2480.5 73 2026.5 74 1534.5 75 1085.0 76 797.0 77 558.5 78 382.0 79 237.0 80 148.5 81 112.0 82 78.0 83 50.5 84 32.5 85 29.5 86 30.0 87 26.0 88 17.0 89 10.0 90 11.5 91 13.0 92 11.0 93 9.5 94 7.5 95 6.5 96 7.5 97 7.0 98 5.0 99 6.5 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036179856062215424 2 0.017761020248723936 3 0.0240296156306265 4 0.02155313350444277 5 0.01354326162756727 6 0.004759489086259355 7 0.005030354318810701 8 0.005765559950021495 9 0.0031342976909512826 10-11 0.004817531636091786 12-13 0.008861162607751157 14-15 0.002747347358735075 16-17 0.002708652325513454 18-19 1.9347516610810387E-5 20-21 0.00450797137031882 22-23 9.093332807080882E-4 24-25 0.0014704112624215895 26-27 0.0017993190448053658 28-29 0.003811460772329646 30-31 0.0013156311295351062 32-33 0.0 34-35 0.006984453496502549 36-37 0.008899857640972777 38-39 0.0017993190448053658 40-41 0.0016832339451405035 42-43 0.0032310352740053346 44-45 0.001644538911918883 46-47 0.021398353371556288 48-49 0.00350190050655668 50-51 0.011260254667491646 52-53 0.0023217019932972463 54-55 1.1608509966486232E-4 56-57 3.2890778238377655E-4 58-59 0.007448793895161998 60-61 6.965105979891739E-4 62-63 0.010815261785443005 64-65 0.01102808446816192 66-67 9.673758305405193E-5 68-69 0.005030354318810701 70-71 0.005455999684248529 72-73 0.012672623380080803 74-75 0.0018380140780269868 76-77 0.0012575885797026752 78-79 6.578155647675531E-4 80-81 0.004662751503205303 82-83 0.0021282268271891423 84-85 0.0013736736793675374 86-87 0.014452594908275359 88-89 0.0 90-91 7.352056312107947E-4 92-93 0.010292878836951125 94-95 0.007797049194156585 96-97 0.005243177001529615 98-99 0.0014510637458107789 100-101 0.016387346569356396 102-103 0.008899857640972777 104-105 0.008261389592816035 106-107 0.018302750713826626 108-109 0.01151177238343218 110-111 0.020392282507794148 112-113 0.0026699572922918332 114-115 0.014974977856767241 116-117 0.006152510282237703 118-119 0.005165786935086373 120-121 0.008609644891810622 122-123 0.01000266608778897 124-125 0.05883579801347438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2584311.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.08243541673111 #Duplication Level Percentage of deduplicated Percentage of total 1 72.93326457273714 38.71475306413683 2 13.545463882602643 14.38052423475837 3 5.305322476509093 8.448583131726776 4 2.654145640787159 5.6355405825473115 5 1.5011248663278078 3.984168188464749 6 0.9564650122808261 3.046289534565593 7 0.6432987585061971 2.390350536144796 8 0.47544398147841527 2.0190179552881182 9 0.3438827647259565 1.6428721184543278 >10 1.5434675465308456 13.990573286592955 >50 0.07043116186145892 2.5300258356186633 >100 0.02570099846418626 2.3571034215679685 >500 0.001693768715964676 0.6203355514535991 >1k 2.945684723387515E-4 0.239862558679871 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.8695033221620775E-5 0.0 12-13 0.0 0.0 0.0 3.8695033221620775E-5 1.9347516610810386E-4 14-15 0.0 0.0 0.0 1.354326162756727E-4 2.3217019932972462E-4 16-17 0.0 0.0 0.0 1.547801328864831E-4 2.708652325513454E-4 18-19 0.0 0.0 0.0 2.1282268271891424E-4 3.095602657729662E-4 20-21 0.0 0.0 0.0 2.902127491621558E-4 4.449928820486389E-4 22-23 0.0 0.0 0.0 5.417304651026908E-4 5.030354318810701E-4 24-25 0.0 0.0 0.0 9.286807973188985E-4 5.030354318810701E-4 26-27 0.0 0.0 0.0 0.0013349786461459168 5.610779817135012E-4 28-29 0.0 0.0 0.0 0.003463205473335059 5.804254983243116E-4 30-31 0.0 0.0 0.0 0.010641134135945713 5.804254983243116E-4 32-33 0.0 0.0 0.0 0.027802381369734525 5.804254983243116E-4 34-35 0.0 0.0 0.0 0.04914269219145838 6.578155647675531E-4 36-37 0.0 0.0 0.0 0.08483886033840354 6.578155647675531E-4 38-39 1.9347516610810387E-5 0.0 0.0 0.14522245968074277 6.578155647675531E-4 40-41 3.8695033221620775E-5 1.9347516610810387E-5 0.0 0.21642132080852497 6.578155647675531E-4 42-43 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.28288004036665865 6.578155647675531E-4 44-45 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.35850948279831646 6.771630813783635E-4 46-47 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.44998454133422794 7.158581145999842E-4 48-49 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.5469542945876096 8.125956976540363E-4 50-51 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.6483933241780885 8.51290730875657E-4 52-53 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.7489617155210808 8.51290730875657E-4 54-55 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.8577334539070569 9.093332807080881E-4 56-57 3.8695033221620775E-5 3.8695033221620775E-5 0.0 0.9817510353823513 0.00100607086376214 58-59 3.8695033221620775E-5 3.8695033221620775E-5 0.0 1.1226783463754944 0.0010834609302053815 60-61 3.8695033221620775E-5 3.8695033221620775E-5 0.0 1.272911812858437 0.0011221559634270024 62-63 3.8695033221620775E-5 3.8695033221620775E-5 0.0 1.4314840590006388 0.001199546029870244 64-65 3.8695033221620775E-5 3.8695033221620775E-5 0.0 1.592378007136138 0.001199546029870244 66-67 3.8695033221620775E-5 3.8695033221620775E-5 0.0 1.7769920106364907 0.0012188935464810544 68-69 3.8695033221620775E-5 3.8695033221620775E-5 0.0 1.9882475445099292 0.0012382410630918648 70-71 3.8695033221620775E-5 3.8695033221620775E-5 0.0 2.220824041688481 0.0012382410630918648 72-73 3.8695033221620775E-5 3.8695033221620775E-5 0.0 2.4515044822391734 0.0012769360963134856 74-75 3.8695033221620775E-5 3.8695033221620775E-5 0.0 2.6843905396834975 0.0013349786461459166 76-77 3.8695033221620775E-5 3.8695033221620775E-5 0.0 2.9438600849510763 0.0013930211959783478 78-79 3.8695033221620775E-5 3.8695033221620775E-5 0.0 3.2399157841296966 0.0014704112624215893 80-81 3.8695033221620775E-5 3.8695033221620775E-5 0.0 3.5576407019124243 0.00150910629564321 82-83 3.8695033221620775E-5 3.8695033221620775E-5 0.0 3.893339462626596 0.0015478013288648309 84-85 3.8695033221620775E-5 3.8695033221620775E-5 0.0 4.229734733938756 0.0015478013288648309 86-87 3.8695033221620775E-5 3.8695033221620775E-5 0.0 4.587296188423142 0.0015864963620864517 88-89 3.8695033221620775E-5 3.8695033221620775E-5 0.0 4.988118690049301 0.0016251913953080725 90-91 3.8695033221620775E-5 3.8695033221620775E-5 0.0 5.421986750046724 0.0016251913953080725 92-93 3.8695033221620775E-5 3.8695033221620775E-5 0.0 5.872126071513838 0.0016638864285296931 94-95 3.8695033221620775E-5 3.8695033221620775E-5 0.0 6.3232134212948825 0.0016638864285296931 96-97 3.8695033221620775E-5 3.8695033221620775E-5 0.0 6.793454812520629 0.001857361594637797 98-99 3.8695033221620775E-5 3.8695033221620775E-5 0.0 7.293549421876856 0.0019734466943026594 100-101 3.8695033221620775E-5 3.8695033221620775E-5 0.0 7.849229446455942 0.0019734466943026594 102-103 3.8695033221620775E-5 3.8695033221620775E-5 0.0 8.420155314124344 0.0020121417275242802 104-105 3.8695033221620775E-5 3.8695033221620775E-5 0.0 8.993441578819269 0.002050836760745901 106-107 3.8695033221620775E-5 3.8695033221620775E-5 0.0 9.579032864078666 0.002089531793967522 108-109 3.8695033221620775E-5 7.739006644324155E-5 0.0 10.176387439437436 0.0021088793105783323 110-111 3.8695033221620775E-5 7.739006644324155E-5 0.0 10.818976508632282 0.002166921860410763 112-113 3.8695033221620775E-5 7.739006644324155E-5 0.0 11.499467362867705 0.002166921860410763 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7185 0.0 33.79984 1 GTACATG 12515 0.0 30.153635 1 CATGGGG 7800 0.0 29.753767 4 TCAACGC 8210 0.0 29.572565 4 CAACGCA 8405 0.0 28.884232 5 TACATGG 12730 0.0 28.70421 2 ATCAACG 8535 0.0 28.65787 3 AACGCAG 8595 0.0 28.242987 6 ACATGGG 12835 0.0 27.727512 3 TATCAAC 8785 0.0 27.639107 2 GGTATCA 5015 0.0 27.417149 1 ACGCAGA 9730 0.0 24.581566 7 CGCAGAG 9910 0.0 23.95497 8 GCAGAGT 10735 0.0 21.28264 9 ATGGGGA 4295 0.0 20.91954 5 GTATAAT 1420 0.0 20.539457 1 ATGGGGG 4510 0.0 20.45001 5 AGAGTAC 9325 0.0 18.982224 10-11 CAGAGTA 10045 0.0 17.740091 10-11 GTGTAGC 1505 0.0 17.401932 1 >>END_MODULE