FastQCFastQC Report
Mon 15 Aug 2016
SRR2920983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584311
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.1533097216240615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.11577553939908934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89450.042.1229361
GGTATCA56900.039.920381
TCAACGC111800.032.994164
TATCAAC114300.032.5306632
CAACGCA113700.032.2860345
ATCAACG114850.032.1173323
AACGCAG117950.031.1178766
GTACATG120900.028.009411
ACGCAGA134450.027.2563617
CATGGGG77550.027.0488664
TACATGG123700.026.6866842
CGCAGAG138250.026.464668
ACATGGG124200.025.9090793
GAGTACT69800.024.12429412-13
ATACGTA1501.6880997E-423.7985215
GCAGAGT149450.023.7648339
AGTACTT73500.023.67893212-13
ACCGTAT4400.022.9854288
CCGTATA2603.0533556E-822.881429
TATACCG2352.913257E-722.7858185
Produced by FastQC (version 0.11.5)