FastQCFastQC Report
Mon 15 Aug 2016
SRR2920982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201021
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71400.039.0930061
GGTATCA45950.037.4314651
TCAACGC80650.034.4535144
CAACGCA81500.034.0195585
ATCAACG82750.033.5811653
AACGCAG85700.032.3507846
TATCAAC89700.031.0456182
GTACATG136000.030.2387331
TACATGG136150.029.2822252
ACATGGG141550.027.5766093
ACGCAGA100400.027.5549077
CGCAGAG104000.026.4294628
CATGGGG95750.026.3480264
GCAGAGT120850.021.4644379
AGAGTAC104300.020.50711810-11
GTATAGG17050.019.1983011
TATACTG24450.018.7375265
TATACCG3253.2147727E-718.3069925
GTATATA27000.018.2952691
CAGAGTA115450.017.93392610-11
Produced by FastQC (version 0.11.5)