FastQCFastQC Report
Mon 15 Aug 2016
SRR2920982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201021
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85100.048.1067161
GGTATCA51350.046.5352971
CAACGCA109850.035.9086045
ATCAACG111200.035.729223
TCAACGC110450.035.7034574
TATCAAC115750.034.8335462
AACGCAG115600.034.4267856
TACATGG129950.028.6898352
GTACATG135150.028.4395161
ACGCAGA140050.028.1643647
CGCAGAG141950.027.4108358
ACATGGG136900.026.6722433
AGAGTAC141300.024.33880210-11
CATGGGG94800.023.8149074
GCAGAGT162650.023.118169
CAGAGTA153250.021.2750369
AGTACTT94550.020.79969812-13
GAGTACT82700.020.11052712-13
GTACTTT95150.017.38533814-15
GTATAGG18700.017.2080121
Produced by FastQC (version 0.11.5)