##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4201021
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.017389582199183	31.0	30.0	34.0	26.0	34.0
2	30.12745568279711	31.0	30.0	34.0	26.0	34.0
3	30.29009781193667	31.0	30.0	34.0	26.0	34.0
4	33.840090539894945	35.0	35.0	37.0	30.0	37.0
5	33.34008208956823	35.0	35.0	37.0	27.0	37.0
6	33.11463951263276	35.0	33.0	37.0	26.0	37.0
7	32.95863695991998	35.0	33.0	37.0	26.0	37.0
8	32.8973192469164	35.0	33.0	37.0	26.0	37.0
9	34.23852368269523	38.0	34.0	39.0	26.0	39.0
10-11	34.14139443720943	38.0	34.0	39.0	25.5	39.0
12-13	34.03376441108007	38.0	33.5	39.0	25.0	39.0
14-15	34.951968699989834	39.0	34.0	41.0	25.0	41.0
16-17	34.94214894426855	39.0	34.0	41.0	25.0	41.0
18-19	34.981832511667996	39.0	34.0	41.0	25.0	41.0
20-21	34.96036487320582	39.0	34.0	41.0	25.0	41.0
22-23	34.91188046905741	39.0	34.0	41.0	25.0	41.0
24-25	34.88854221390467	39.0	34.0	41.0	24.0	41.0
26-27	34.74732678079924	39.0	34.0	41.0	24.0	41.0
28-29	34.63810928343372	39.0	34.0	41.0	23.5	41.0
30-31	34.482032701098134	38.5	33.5	41.0	22.5	41.0
32-33	34.36394426497749	38.0	33.0	41.0	22.0	41.0
34-35	34.24441522667942	38.0	33.0	41.0	21.5	41.0
36-37	34.10972285070701	38.0	33.0	40.5	19.5	41.0
38-39	33.98031228598953	38.0	33.0	40.0	19.0	41.0
40-41	33.81206187733886	38.0	33.0	40.0	17.0	41.0
42-43	33.6798943637749	38.0	33.0	40.0	16.0	41.0
44-45	33.47275067180097	38.0	32.5	40.0	15.0	41.0
46-47	33.349603584461974	38.0	32.5	40.0	14.0	41.0
48-49	33.16230661546324	38.0	32.0	40.0	9.5	41.0
50-51	32.97938882000352	38.0	32.0	40.0	9.0	41.0
52-53	32.75829566193552	38.0	31.5	40.0	8.0	41.0
54-55	32.596172930342405	37.5	31.0	40.0	7.5	41.0
56-57	32.48958515084786	37.0	31.0	40.0	7.0	41.0
58-59	32.4515515394948	37.0	31.0	40.0	4.0	41.0
60-61	32.214749581113736	37.0	31.0	40.0	2.0	41.0
62-63	31.981946412550663	36.5	31.0	40.0	2.0	41.0
64-65	31.736155805933844	36.0	31.0	39.5	2.0	41.0
66-67	31.43963217513076	36.0	30.5	39.0	2.0	41.0
68-69	31.134433867386047	35.0	30.0	39.0	2.0	41.0
70-71	30.787898822690963	35.0	30.0	38.5	2.0	40.0
72-73	30.5046460134334	35.0	30.0	37.5	2.0	40.0
74-75	30.226672397019676	35.0	29.5	37.0	2.0	39.0
76-77	29.87534482688851	35.0	29.0	37.0	2.0	39.0
78-79	29.52375374938616	34.5	29.0	36.0	2.0	39.0
80-81	29.229665835995583	34.0	29.0	36.0	2.0	37.5
82-83	28.943102283944782	34.0	29.0	35.0	2.0	37.0
84-85	28.71114367197879	34.0	29.0	35.0	2.0	37.0
86-87	28.455670538185835	34.0	29.0	35.0	2.0	36.0
88-89	28.30920090616067	34.0	28.5	35.0	2.0	36.0
90-91	28.100419040990275	34.0	28.0	35.0	2.0	36.0
92-93	28.22075942967198	34.0	29.0	35.0	2.0	35.0
94-95	28.152985548037012	34.0	29.0	35.0	2.0	35.0
96-97	28.08192639360765	34.0	29.0	35.0	2.0	35.0
98-99	28.03311825863284	34.0	29.0	35.0	2.0	35.0
100-101	27.898246402481682	34.0	28.0	35.0	2.0	35.0
102-103	26.601113038949343	32.0	25.0	34.0	2.0	35.0
104-105	27.65576070674248	34.0	27.0	35.0	2.0	35.0
106-107	27.849269141953826	34.0	29.0	35.0	2.0	35.0
108-109	27.829131061234875	34.0	29.0	35.0	2.0	35.0
110-111	27.768575781934913	34.0	28.5	35.0	2.0	35.0
112-113	27.66220378331839	34.0	27.5	35.0	2.0	35.0
114-115	27.573776112997294	34.0	27.0	35.0	2.0	35.0
116-117	27.446466704165488	34.0	27.0	35.0	2.0	35.0
118-119	27.31875846371632	34.0	27.0	35.0	2.0	35.0
120-121	27.1832131998388	34.0	27.0	35.0	2.0	35.0
122-123	26.95759804580839	34.0	27.0	35.0	2.0	35.0
124-125	25.841133976716613	33.0	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	137637.0
3	45140.0
4	21738.0
5	16612.0
6	17873.0
7	20833.0
8	23722.0
9	25712.0
10	27161.0
11	28475.0
12	29485.0
13	31707.0
14	34009.0
15	29669.0
16	24143.0
17	21471.0
18	19952.0
19	18784.0
20	18859.0
21	19556.0
22	22117.0
23	27973.0
24	37271.0
25	49690.0
26	61344.0
27	71512.0
28	85011.0
29	99820.0
30	118119.0
31	140912.0
32	171855.0
33	212410.0
34	272298.0
35	372480.0
36	588453.0
37	903448.0
38	352988.0
39	782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.85060589540841	20.87354396835116	14.31587917292139	24.95997096331904
2	15.085374585576442	21.156667815595085	39.25264745480617	24.505310144022303
3	17.774025681572404	25.245357495076664	30.78018077507474	26.20043604827619
4	12.733764783582485	17.343337106318334	37.47470276719729	32.44819534290189
5	13.130795363019146	38.05148428670505	35.28437589057559	13.533344459700212
6	31.503864638852058	37.80052417214365	17.477237074824263	13.218374114180023
7	27.278193799093643	32.69743449598443	22.103074838845135	17.92129686607679
8	25.178240685632662	36.67534817283657	20.353695988572788	17.79271515295798
9	25.866372938953404	15.788817012587241	20.059178895797142	38.28563115266222
10-11	24.421041754611274	26.55418804420004	28.77614201801058	20.24862818317811
12-13	25.001836240664783	23.919126842267225	28.73764852865637	22.341388388411627
14-15	22.21842042853755	25.46397756830609	26.151145824106642	26.166456179049717
16-17	22.24271606875661	28.51892911866286	26.830530388712482	22.407824423868046
18-19	22.160771817506586	27.687867135673173	28.504826240804565	21.646534806015673
20-21	23.12549843079492	26.858733924174594	28.16443064515918	21.85133699987131
22-23	22.69861940795579	26.73808537125294	27.951283502145905	22.612011718645366
24-25	22.279664694268337	26.90243384152203	28.241085368677375	22.576816095532255
26-27	22.31087895760441	27.15759133514734	28.067832759477195	22.463696947771055
28-29	22.39140680364947	27.155230447939083	28.132977835003743	22.320384913407697
30-31	22.58506959904417	26.87125260183899	28.097777716400856	22.44590008271598
32-33	22.419563712711067	27.01408282358387	27.925048435227275	22.641305028477795
34-35	22.57819725108363	27.0505556978916	27.97970314522435	22.39154390580042
36-37	22.643015616098637	26.878857815698726	27.996028261727552	22.482098306475088
38-39	22.5111973616849	27.040765301870394	27.954448585001128	22.49358875144357
40-41	22.71785560060135	26.92975574665259	27.87589204423756	22.4764966085085
42-43	22.656470256554833	26.80928258749305	27.87030008229261	22.663947073659507
44-45	22.665723119225927	26.929520982600526	27.77652307450902	22.628232823664533
46-47	22.70829600056676	26.988491486343204	27.541228769438586	22.761983743651445
48-49	22.60707427931369	27.122269507058753	27.530938601888554	22.739717611739
50-51	22.713803027095956	27.173208850764013	27.4135779556138	22.699410166526228
52-53	22.785324027735825	27.0710286188674	27.40627919483191	22.737368158564866
54-55	22.671340885184595	27.06035587637728	27.424840149703577	22.843463088734552
56-57	22.62745298323548	27.175977398571376	27.331063999800946	22.8655056183922
58-59	22.47039263576528	27.159520519934784	27.495182757575883	22.874904086724055
60-61	22.67048869727403	27.161239713428674	27.440890615370105	22.72738097392719
62-63	22.442549273505016	27.205890933703657	27.473808956195008	22.877750836596324
64-65	22.365576955569836	27.10695200601171	27.433673629162918	23.093797409255536
66-67	22.5153142967092	27.247267668473935	27.21358383595152	23.023834198865348
68-69	22.527107316698583	27.048801206264432	27.347688578525947	23.076402898511038
70-71	22.429009785086247	26.929597004561543	27.420268168322913	23.221125042029296
72-73	22.506126860755266	26.90122594366991	27.336675686742417	23.255971508832406
74-75	22.4684098106672	26.989287276375794	27.18756302144854	23.354739891508462
76-77	22.44053834883058	26.782135291000074	27.36302594556037	23.41430041460898
78-79	22.486473267872277	26.87872337841728	27.27198262139651	23.36282073231393
80-81	22.425367328471612	27.00283467424104	27.142919558514162	23.42887843877319
82-83	22.41127156869364	26.871812969857555	27.30922647570353	23.407688985745274
84-85	22.45376521041008	26.8434750352612	27.16953994253448	23.53321981179424
86-87	22.367293735644253	26.693769647994614	27.259331859837914	23.67960475652322
88-89	22.32166796759846	26.651544425908085	27.326258931305563	23.70052867518789
90-91	22.201044987019817	26.74138309570856	27.35207804398547	23.70549387328616
92-93	22.23043673970325	26.624286899523238	27.34962879309809	23.79564756767542
94-95	22.170900142974318	26.553009424876578	27.416188597530272	23.85990183461883
96-97	22.084853333047636	26.480630010493428	27.454275015936563	23.980241640522372
98-99	22.042222911917325	26.485506224101464	27.32339053318471	24.1488803307965
100-101	21.91235073989867	26.518339310761252	27.425453580944804	24.143856368395276
102-103	21.882254421301727	26.397399691581246	27.39967134223588	24.320674544881143
104-105	21.8557441101732	26.49239005626876	27.279188415528104	24.372677418029934
106-107	21.7532590433886	26.510723173496547	27.221324024989112	24.514693758125734
108-109	21.78670742624231	26.403348380092567	27.271846370683576	24.538097822981545
110-111	21.645813229295907	26.488329717880386	27.197146589771926	24.668710463051784
112-113	21.570395242585704	26.585509160603205	27.214813329959075	24.629282266852012
114-115	21.532446288100548	26.60980949323384	27.15584738841375	24.70189683025186
116-117	21.496879353739587	26.45820219540369	27.182857300664793	24.862061150191934
118-119	21.426272475581275	26.466468444319595	27.122566162064622	24.984692918034504
120-121	21.424291599942773	26.517751868764982	27.15677772331685	24.901178807975395
122-123	21.33157305193588	26.488727799547878	27.04189051555955	25.13780863295669
124-125	21.139436391746646	26.56929347745175	26.956637630742946	25.334632500058657
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	74.5
2	181.0
3	300.0
4	286.0
5	223.5
6	269.0
7	529.5
8	801.5
9	826.5
10	725.5
11	656.5
12	711.0
13	832.0
14	967.0
15	1161.5
16	1367.0
17	1585.0
18	1820.0
19	2150.0
20	2692.0
21	3312.5
22	4139.0
23	5458.5
24	7108.0
25	9469.0
26	13167.0
27	18007.0
28	23583.5
29	30880.0
30	39424.0
31	48967.5
32	59827.5
33	70528.0
34	81243.0
35	91459.5
36	103208.0
37	115111.5
38	124523.5
39	132433.0
40	140882.5
41	149350.5
42	157095.5
43	164979.5
44	169951.0
45	172835.0
46	175481.5
47	176827.5
48	177223.0
49	176854.0
50	173419.5
51	166433.5
52	157162.0
53	147845.5
54	136986.0
55	123349.0
56	108515.0
57	95279.5
58	85408.0
59	75594.5
60	63166.5
61	50395.5
62	39329.5
63	29285.0
64	21418.5
65	16035.5
66	12171.0
67	9361.0
68	7305.0
69	5474.5
70	3991.5
71	3043.5
72	2391.0
73	1847.0
74	1365.0
75	1028.5
76	727.0
77	421.0
78	234.0
79	164.0
80	117.5
81	62.5
82	44.5
83	25.0
84	14.0
85	11.5
86	7.5
87	7.0
88	3.5
89	3.0
90	5.5
91	5.0
92	5.0
93	7.0
94	7.5
95	8.5
96	10.5
97	10.0
98	5.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05222539949217107
2	1.1901868617176634E-4
3	0.0026184110957788594
4	0.12863539601444507
5	0.018757344940670377
6	0.002642214833013213
7	0.00590332683411961
8	0.012401747099098054
9	0.02887393326527051
10-11	0.02223269057688595
12-13	0.020554527101864044
14-15	0.02973086780570723
16-17	0.03945469446594054
18-19	0.04260868964949235
20-21	0.022958704562533724
22-23	0.02493441475298505
24-25	0.023172938197642906
26-27	0.022054162547628302
28-29	0.021601891540175593
30-31	0.026350737118429068
32-33	0.02575564368757024
34-35	0.029921297703582057
36-37	0.03209933966052538
38-39	0.03263492374829833
40-41	0.03493198439141342
42-43	0.032682531222767035
44-45	0.030171236944542767
46-47	0.041382797181923156
48-49	0.033634680712141166
50-51	0.02432741945350904
52-53	0.031575657441369614
54-55	0.03305148914989951
56-57	0.026410246461514953
58-59	0.030076021995605356
60-61	0.04420354004419402
62-63	0.051463679900671765
64-65	0.04476292786920132
66-67	0.03991886734201043
68-69	0.030433078054120655
70-71	0.03734806372070028
72-73	0.04009739537126808
74-75	0.025279568942883173
76-77	0.021875634518370653
78-79	0.032706334960001394
80-81	0.025410489497672117
82-83	0.03990696547339325
84-85	0.02230410178858901
86-87	0.03530094231854589
88-89	0.03492008252279624
90-91	0.03672916655260709
92-93	0.027124358578545546
94-95	0.022994410168385257
96-97	0.018412190750772252
98-99	0.02612460161470271
100-101	0.025089139045008344
102-103	0.028838227659418982
104-105	0.02591036797959353
106-107	0.015043961932111266
108-109	0.0185907187800299
110-111	0.023660914810947148
112-113	0.013996597493799723
114-115	0.01598420955286822
116-117	0.014496475975721141
118-119	0.008355111769257996
120-121	0.005998541783057023
122-123	0.004225163359097705
124-125	0.07011390802378756
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4201021.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.865458403374596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49043267666003	44.43778924580681
2	13.563148734369236	15.9680193528359
3	4.784941593047497	8.450033409243431
4	2.1707227187229137	5.111223516169756
5	1.1576719503001347	3.4073445017573056
6	0.7000398884655846	2.472490136110432
7	0.44939581527244593	1.8517723469399543
8	0.3070133265340242	1.445798416189621
9	0.2238272962048647	1.1858126754858538
>10	1.0792918157346076	10.952386929958429
>50	0.05208332250723906	2.0838273693268548
>100	0.020701908098672846	2.1141932287289387
>500	4.8596947121712857E-4	0.19242849116396532
>1k	2.4298461144133494E-4	0.3268803802827386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.760747446870654E-5	0.0	0.0	0.0	0.0
3	4.760747446870654E-5	0.0	0.0	0.0	0.0
4	4.760747446870654E-5	0.0	0.0	0.0	0.0
5	7.14112117030598E-5	0.0	0.0	0.0	0.0
6	7.14112117030598E-5	0.0	0.0	0.0	2.380373723435327E-5
7	1.1901868617176634E-4	0.0	0.0	0.0	2.380373723435327E-5
8	1.428224234061196E-4	0.0	0.0	2.380373723435327E-5	2.380373723435327E-5
9	1.428224234061196E-4	0.0	0.0	2.380373723435327E-5	2.380373723435327E-5
10-11	1.428224234061196E-4	0.0	0.0	4.760747446870654E-5	2.380373723435327E-5
12-13	1.5472429202329624E-4	0.0	0.0	7.14112117030598E-5	4.760747446870654E-5
14-15	1.9042989787482615E-4	0.0	0.0	7.14112117030598E-5	5.9509343085883165E-5
16-17	1.9042989787482615E-4	0.0	0.0	7.14112117030598E-5	7.14112117030598E-5
18-19	2.6184110957788595E-4	0.0	0.0	1.1901868617176634E-4	9.521494893741308E-5
20-21	2.856448468122392E-4	0.0	0.0	1.785280292576495E-4	1.1901868617176634E-4
22-23	3.094485840465925E-4	0.0	0.0	3.332523212809458E-4	1.428224234061196E-4
24-25	3.808597957496523E-4	0.0	0.0	6.307990367103616E-4	1.428224234061196E-4
26-27	3.9276166436682896E-4	0.0	0.0	9.75953226608484E-4	1.666261606404729E-4
28-29	5.593878250073018E-4	0.0	0.0	0.0030468783659972185	1.666261606404729E-4
30-31	6.903083797962447E-4	0.0	0.0	0.011473401346958276	1.7852802925764953E-4
32-33	7.14112117030598E-4	0.0	0.0	0.03186130228818185	2.1423363510917942E-4
34-35	7.974251973508345E-4	0.0	0.0	0.05767645531883797	2.3803737234353269E-4
36-37	8.807382776710709E-4	0.0	0.0	0.10215373834122704	2.3803737234353269E-4
38-39	8.807382776710709E-4	0.0	0.0	0.171351202481492	2.6184110957788595E-4
40-41	8.926401462882475E-4	0.0	0.0	0.24839199804047635	2.6184110957788595E-4
42-43	9.40247620756954E-4	0.0	0.0	0.32587316273829625	2.737429781950626E-4
44-45	0.0010235607010771906	0.0	0.0	0.41588699509000315	3.094485840465925E-4
46-47	0.0011782849931004868	0.0	0.0	0.5194570557966742	3.332523212809458E-4
48-49	0.0012854018106550765	0.0	0.0	0.6316202656449468	3.4515418989812237E-4
50-51	0.0012854018106550765	0.0	0.0	0.7467589426475135	3.808597957496523E-4
52-53	0.0012973036792722531	0.0	0.0	0.8613144280878386	4.2846727021835884E-4
54-55	0.0013092055478894297	0.0	0.0	0.9919731417672037	4.2846727021835884E-4
56-57	0.0014520279712955492	0.0	0.0	1.1447812329431346	4.4036913883553543E-4
58-59	0.0014520279712955492	0.0	0.0	1.305980141494175	4.522710074527121E-4
60-61	0.0014520279712955492	0.0	0.0	1.4737488815218966	4.522710074527121E-4
62-63	0.0014520279712955492	0.0	0.0	1.6441479345140146	4.7607474468706537E-4
64-65	0.0014520279712955492	0.0	0.0	1.8294481270148375	4.7607474468706537E-4
66-67	0.0015710466574673156	0.0	0.0	2.039837458560669	4.7607474468706537E-4
68-69	0.001594850394701669	0.0	0.0	2.272304756391363	4.7607474468706537E-4
70-71	0.0016424578691703754	0.0	0.0	2.5217322170015337	4.7607474468706537E-4
72-73	0.0016662616064047288	0.0	0.0	2.772290355130336	5.117803505385953E-4
74-75	0.0016662616064047288	0.0	0.0	3.0359643524752675	5.474859563901252E-4
76-77	0.0016662616064047288	0.0	0.0	3.329666764341335	5.474859563901252E-4
78-79	0.0016781634750219054	0.0	0.0	3.6575156372700826	5.593878250073018E-4
80-81	0.0018328877670452017	0.0	0.0	3.9982780376484666	5.712896936244784E-4
82-83	0.001856691504279555	0.0	0.0	4.351346970177012	5.712896936244784E-4
84-85	0.0019042989787482615	0.0	0.0	4.718781458126489	5.712896936244784E-4
86-87	0.0019638083218341445	0.0	0.0	5.114232944800799	5.712896936244784E-4
88-89	0.0019757101904513213	0.0	0.0	5.552757294000672	5.712896936244784E-4
90-91	0.0020947288766230876	0.0	0.0	6.020167478334433	5.712896936244784E-4
92-93	0.002142336351091794	0.0	0.0	6.498075110788544	5.712896936244784E-4
94-95	0.002142336351091794	0.0	0.0	6.983207177493281	5.712896936244784E-4
96-97	0.002142336351091794	0.0	0.0	7.495451700907946	6.069952994760084E-4
98-99	0.002237551300029207	0.0	0.0	8.051102339169454	6.18897168093185E-4
100-101	0.0023565699862009735	0.0	0.0	8.637745443310092	6.18897168093185E-4
102-103	0.00247558867237274	0.0	0.0	9.244883565209506	6.18897168093185E-4
104-105	0.0026779204388647426	0.0	0.0	9.864768588397915	6.307990367103616E-4
106-107	0.0027731353878021558	0.0	0.0	10.496936339999252	6.427009053275383E-4
108-109	0.0028445465995052153	0.0	0.0	11.172343580286793	6.427009053275383E-4
110-111	0.0028802522053567453	0.0	0.0	11.876815183737477	6.665046425618916E-4
112-113	0.0029159578112082753	0.0	0.0	12.59819934249317	7.14112117030598E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8510	0.0	48.106716	1
GGTATCA	5135	0.0	46.535297	1
CAACGCA	10985	0.0	35.908604	5
ATCAACG	11120	0.0	35.72922	3
TCAACGC	11045	0.0	35.703457	4
TATCAAC	11575	0.0	34.833546	2
AACGCAG	11560	0.0	34.426785	6
TACATGG	12995	0.0	28.689835	2
GTACATG	13515	0.0	28.439516	1
ACGCAGA	14005	0.0	28.164364	7
CGCAGAG	14195	0.0	27.410835	8
ACATGGG	13690	0.0	26.672243	3
AGAGTAC	14130	0.0	24.338802	10-11
CATGGGG	9480	0.0	23.814907	4
GCAGAGT	16265	0.0	23.11816	9
CAGAGTA	15325	0.0	21.275036	9
AGTACTT	9455	0.0	20.799698	12-13
GAGTACT	8270	0.0	20.110527	12-13
GTACTTT	9515	0.0	17.385338	14-15
GTATAGG	1870	0.0	17.208012	1
>>END_MODULE