FastQCFastQC Report
Mon 15 Aug 2016
SRR2920981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414168
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29860.1236865040046923No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28440.11780456041170291No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG54950.026.747834
GTACATG115450.024.6439441
TACATGG117400.023.7217142
GTATCAA81250.022.4900951
ACATGGG122100.022.321233
TCAACGC88200.020.1726024
CAACGCA90000.020.0323755
ATCAACG88400.019.9927483
AACGCAG89500.019.9419466
ATGGGGA31600.019.7712695
TATCAAC91500.019.5755372
GTGTAGC17000.019.2570631
GTATATA15150.019.2512841
GTATAGG6200.019.2005921
CGTATAC1550.005686059619.1958183
ACGCAGA97650.018.1557147
TAGGACG4401.375156E-917.5812594
CGCAGAG100250.017.4474648
TAGCCCT21050.017.2439614
TACCCCG3506.9822636E-717.00065
Produced by FastQC (version 0.11.5)